Brain-derived neurotrophic factor modulates immune reaction in mice with peripheral nerve xenotransplantation

Xin Yu,1 Laijin Lu,1 Zhigang Liu,1 Teng Yang,2 Xu Gong,1 Yubo Ning,3 Yanfang Jiang4 1Department of Hand Surgery, 2Department of Orthopedics, The First Hospital of Jilin University, Changchun, 3Department of Orthopedics, Ningshi Orthopedics Hospital of Tonghua, Tonghua, 4Department of Central Labora...

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Autores principales:	Yu X, Lu L, Liu Z, Yang T, Gong X, Ning Y, Jiang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Peripheral nerve Xenotransplantation Brain-derived neurotrophic factor Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/6f7569341ab94bee8d94c4484a16b0f8
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spelling	oai:doaj.org-article:6f7569341ab94bee8d94c4484a16b0f82021-12-02T04:36:11ZBrain-derived neurotrophic factor modulates immune reaction in mice with peripheral nerve xenotransplantation1178-2021https://doaj.org/article/6f7569341ab94bee8d94c4484a16b0f82016-03-01T00:00:00Zhttps://www.dovepress.com/brain-derived-neurotrophic-factor-modulates-immune-reaction-in-mice-wi-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Xin Yu,1 Laijin Lu,1 Zhigang Liu,1 Teng Yang,2 Xu Gong,1 Yubo Ning,3 Yanfang Jiang4 1Department of Hand Surgery, 2Department of Orthopedics, The First Hospital of Jilin University, Changchun, 3Department of Orthopedics, Ningshi Orthopedics Hospital of Tonghua, Tonghua, 4Department of Central Laboratory, The First Hospital of Jilin University, Changchun, People’s Republic of China Background: Brain-derived neurotrophic factor (BDNF) has been demonstrated to play an important role in survival, differentiation, and neurite outgrowth for many types of neurons. This study was designed to identify the role of BDNF during peripheral nerve xenotransplantation. Materials and methods: A peripheral nerve xenotransplantation from rats to mice was performed. Intracellular cytokines were stained for natural killer (NK) cells, natural killer T (NKT) cells, T cells, and B cells and analyzed by flow cytometry in the spleen of the recipient mouse. Serum levels of related cytokines were quantified by cytometric bead array. Results: Splenic NK cells significantly increased in the xenotransplanted mice (8.47±0.88×107 cells/mL) compared to that in the control mice (4.66±0.78×107 cells/mL, P=0.0003), which significantly reduced in the presence of BDNF (4.85±0.87×107 cells/mL, P=0.0004). In contrast, splenic NKT cell number was significantly increased in the mice with xenotransplantation plus BDNF (XT + BDNF) compared to that of control group or of mice receiving xenotransplantation only (XT only). Furthermore, the number of CD3+ T cells, CD3+CD4+ T cells, CD3+CD4- T cells, interferon-γ-producing CD3+CD4+ T cells, and interleukin (IL)-17-producing CD3+CD4+ T cells, as well as CD3-CD19+ B cells, was significantly higher in the spleen of XT only mice compared to the control mice (P<0.05), which was significantly reduced by BDNF (P<0.05). The number of IL-4-producing CD3+CD4+ T cells and CD3+CD4+CD25+Foxp3+ T cells was significantly higher in the spleen of XT + BDNF mice than that in the spleen of XT only mice (P<0.05). Serum levels of IL-6, TNF-α, interferon-γ, and IL-17 were decreased, while IL-4 and IL-10 were stimulated by BDNF following xenotransplantation. Conclusion: BDNF reduced NK cells but increased NKT cell accumulation in the spleen of xenotransplanted mice. BDNF modulated the number of splenic T cells and its subtype cells in the mice following xenotransplantation. These findings suggest that BDNF inhibits rejection of peripheral nerve following xenotransplantation by regulating innate as well as adaptive immune reaction. Keywords: peripheral nerve, xenotransplantation, brain-derived neurotrophic factorYu XLu LLiu ZYang TGong XNing YJiang YDove Medical PressarticlePeripheral nerveXenotransplantationBrain-derived neurotrophic factorNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol 2016, Iss Issue 1, Pp 685-694 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Peripheral nerve Xenotransplantation Brain-derived neurotrophic factor Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Peripheral nerve Xenotransplantation Brain-derived neurotrophic factor Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Yu X Lu L Liu Z Yang T Gong X Ning Y Jiang Y Brain-derived neurotrophic factor modulates immune reaction in mice with peripheral nerve xenotransplantation
description	Xin Yu,1 Laijin Lu,1 Zhigang Liu,1 Teng Yang,2 Xu Gong,1 Yubo Ning,3 Yanfang Jiang4 1Department of Hand Surgery, 2Department of Orthopedics, The First Hospital of Jilin University, Changchun, 3Department of Orthopedics, Ningshi Orthopedics Hospital of Tonghua, Tonghua, 4Department of Central Laboratory, The First Hospital of Jilin University, Changchun, People’s Republic of China Background: Brain-derived neurotrophic factor (BDNF) has been demonstrated to play an important role in survival, differentiation, and neurite outgrowth for many types of neurons. This study was designed to identify the role of BDNF during peripheral nerve xenotransplantation. Materials and methods: A peripheral nerve xenotransplantation from rats to mice was performed. Intracellular cytokines were stained for natural killer (NK) cells, natural killer T (NKT) cells, T cells, and B cells and analyzed by flow cytometry in the spleen of the recipient mouse. Serum levels of related cytokines were quantified by cytometric bead array. Results: Splenic NK cells significantly increased in the xenotransplanted mice (8.47±0.88×107 cells/mL) compared to that in the control mice (4.66±0.78×107 cells/mL, P=0.0003), which significantly reduced in the presence of BDNF (4.85±0.87×107 cells/mL, P=0.0004). In contrast, splenic NKT cell number was significantly increased in the mice with xenotransplantation plus BDNF (XT + BDNF) compared to that of control group or of mice receiving xenotransplantation only (XT only). Furthermore, the number of CD3+ T cells, CD3+CD4+ T cells, CD3+CD4- T cells, interferon-γ-producing CD3+CD4+ T cells, and interleukin (IL)-17-producing CD3+CD4+ T cells, as well as CD3-CD19+ B cells, was significantly higher in the spleen of XT only mice compared to the control mice (P<0.05), which was significantly reduced by BDNF (P<0.05). The number of IL-4-producing CD3+CD4+ T cells and CD3+CD4+CD25+Foxp3+ T cells was significantly higher in the spleen of XT + BDNF mice than that in the spleen of XT only mice (P<0.05). Serum levels of IL-6, TNF-α, interferon-γ, and IL-17 were decreased, while IL-4 and IL-10 were stimulated by BDNF following xenotransplantation. Conclusion: BDNF reduced NK cells but increased NKT cell accumulation in the spleen of xenotransplanted mice. BDNF modulated the number of splenic T cells and its subtype cells in the mice following xenotransplantation. These findings suggest that BDNF inhibits rejection of peripheral nerve following xenotransplantation by regulating innate as well as adaptive immune reaction. Keywords: peripheral nerve, xenotransplantation, brain-derived neurotrophic factor
format	article
author	Yu X Lu L Liu Z Yang T Gong X Ning Y Jiang Y
author_facet	Yu X Lu L Liu Z Yang T Gong X Ning Y Jiang Y
author_sort	Yu X
title	Brain-derived neurotrophic factor modulates immune reaction in mice with peripheral nerve xenotransplantation
title_short	Brain-derived neurotrophic factor modulates immune reaction in mice with peripheral nerve xenotransplantation
title_full	Brain-derived neurotrophic factor modulates immune reaction in mice with peripheral nerve xenotransplantation
title_fullStr	Brain-derived neurotrophic factor modulates immune reaction in mice with peripheral nerve xenotransplantation
title_full_unstemmed	Brain-derived neurotrophic factor modulates immune reaction in mice with peripheral nerve xenotransplantation
title_sort	brain-derived neurotrophic factor modulates immune reaction in mice with peripheral nerve xenotransplantation
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/6f7569341ab94bee8d94c4484a16b0f8
work_keys_str_mv	AT yux brainderivedneurotrophicfactormodulatesimmunereactioninmicewithperipheralnervexenotransplantation AT lul brainderivedneurotrophicfactormodulatesimmunereactioninmicewithperipheralnervexenotransplantation AT liuz brainderivedneurotrophicfactormodulatesimmunereactioninmicewithperipheralnervexenotransplantation AT yangt brainderivedneurotrophicfactormodulatesimmunereactioninmicewithperipheralnervexenotransplantation AT gongx brainderivedneurotrophicfactormodulatesimmunereactioninmicewithperipheralnervexenotransplantation AT ningy brainderivedneurotrophicfactormodulatesimmunereactioninmicewithperipheralnervexenotransplantation AT jiangy brainderivedneurotrophicfactormodulatesimmunereactioninmicewithperipheralnervexenotransplantation
_version_	1718401125424562176

Brain-derived neurotrophic factor modulates immune reaction in mice with peripheral nerve xenotransplantation

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