Elevation of plasma tRNA fragments as a promising biomarker for liver fibrosis in nonalcoholic fatty liver disease

Abstract Fibrotic tissue remodelling in nonalcoholic fatty liver disease (NAFLD) will probably emerge as the leading cause of end-stage liver disease in the coming decades, but the ability to diagnose liver fibrosis in NAFLD patients noninvasively is limited. The abnormal expression of tRNA-derived...

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Autores principales:	Peng Huang, Biao Tu, Hui-jun Liao, Fei-zhou Huang, Zhen-zhou Li, Kuang-ye Zhu, Feng Dai, Huai-zheng Liu, Tian-yi Zhang, Chuan-zheng Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f7b729e989f40e4bfaca615c3cff45c
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spelling	oai:doaj.org-article:6f7b729e989f40e4bfaca615c3cff45c2021-12-02T17:05:11ZElevation of plasma tRNA fragments as a promising biomarker for liver fibrosis in nonalcoholic fatty liver disease10.1038/s41598-021-85421-02045-2322https://doaj.org/article/6f7b729e989f40e4bfaca615c3cff45c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85421-0https://doaj.org/toc/2045-2322Abstract Fibrotic tissue remodelling in nonalcoholic fatty liver disease (NAFLD) will probably emerge as the leading cause of end-stage liver disease in the coming decades, but the ability to diagnose liver fibrosis in NAFLD patients noninvasively is limited. The abnormal expression of tRNA-derived small RNA (tsRNA) in plasma provides a novel idea for noninvasive diagnosis of various diseases, however, the relationship between tsRNAs and NAFLD is still unknown. Here, we took advantage of small RNA-Seq technology to profile tsRNAs in NAFLD patients and found the ubiquitous presence of hepatic tsRNAs secreted into circulating blood. Verification in a cohort of 114 patients with NAFLD and 42 patients without NAFLD revealed that three tsRNAs (tRF-Val-CAC-005, tiRNA-His-GTG-001, and tRF-Ala-CGC-006) were significantly elevated in the plasma of NAFLD patients, and the expression level are associated with NAFLD activity score (calculated from 0 to 8) and fibrosis stage (scored from 0 to 4). In mouse models, we further found that increased plasma levels of these three tsRNAs were positively correlated with the degree of liver fibrosis. Our study potentially identifies a new class of NAFLD biomarkers and reveal the possible existence of tsRNAs in the blood that can be used to predict fibrogenesis risk in patients diagnosed with NAFLD.Peng HuangBiao TuHui-jun LiaoFei-zhou HuangZhen-zhou LiKuang-ye ZhuFeng DaiHuai-zheng LiuTian-yi ZhangChuan-zheng SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Huang Biao Tu Hui-jun Liao Fei-zhou Huang Zhen-zhou Li Kuang-ye Zhu Feng Dai Huai-zheng Liu Tian-yi Zhang Chuan-zheng Sun Elevation of plasma tRNA fragments as a promising biomarker for liver fibrosis in nonalcoholic fatty liver disease
description	Abstract Fibrotic tissue remodelling in nonalcoholic fatty liver disease (NAFLD) will probably emerge as the leading cause of end-stage liver disease in the coming decades, but the ability to diagnose liver fibrosis in NAFLD patients noninvasively is limited. The abnormal expression of tRNA-derived small RNA (tsRNA) in plasma provides a novel idea for noninvasive diagnosis of various diseases, however, the relationship between tsRNAs and NAFLD is still unknown. Here, we took advantage of small RNA-Seq technology to profile tsRNAs in NAFLD patients and found the ubiquitous presence of hepatic tsRNAs secreted into circulating blood. Verification in a cohort of 114 patients with NAFLD and 42 patients without NAFLD revealed that three tsRNAs (tRF-Val-CAC-005, tiRNA-His-GTG-001, and tRF-Ala-CGC-006) were significantly elevated in the plasma of NAFLD patients, and the expression level are associated with NAFLD activity score (calculated from 0 to 8) and fibrosis stage (scored from 0 to 4). In mouse models, we further found that increased plasma levels of these three tsRNAs were positively correlated with the degree of liver fibrosis. Our study potentially identifies a new class of NAFLD biomarkers and reveal the possible existence of tsRNAs in the blood that can be used to predict fibrogenesis risk in patients diagnosed with NAFLD.
format	article
author	Peng Huang Biao Tu Hui-jun Liao Fei-zhou Huang Zhen-zhou Li Kuang-ye Zhu Feng Dai Huai-zheng Liu Tian-yi Zhang Chuan-zheng Sun
author_facet	Peng Huang Biao Tu Hui-jun Liao Fei-zhou Huang Zhen-zhou Li Kuang-ye Zhu Feng Dai Huai-zheng Liu Tian-yi Zhang Chuan-zheng Sun
author_sort	Peng Huang
title	Elevation of plasma tRNA fragments as a promising biomarker for liver fibrosis in nonalcoholic fatty liver disease
title_short	Elevation of plasma tRNA fragments as a promising biomarker for liver fibrosis in nonalcoholic fatty liver disease
title_full	Elevation of plasma tRNA fragments as a promising biomarker for liver fibrosis in nonalcoholic fatty liver disease
title_fullStr	Elevation of plasma tRNA fragments as a promising biomarker for liver fibrosis in nonalcoholic fatty liver disease
title_full_unstemmed	Elevation of plasma tRNA fragments as a promising biomarker for liver fibrosis in nonalcoholic fatty liver disease
title_sort	elevation of plasma trna fragments as a promising biomarker for liver fibrosis in nonalcoholic fatty liver disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6f7b729e989f40e4bfaca615c3cff45c
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Elevation of plasma tRNA fragments as a promising biomarker for liver fibrosis in nonalcoholic fatty liver disease

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