In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis

<h4>Purpose</h4> To assess the in vitro larvicidal activity of ivermectin and povidone-iodine (PVP-I) against Oestrus ovis, the most frequent cause of external ophthalmomyiasis. <h4>Methods</h4> L1 O. ovis larvae were collected from the nasal boots of sheep slaughtered in loc...

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Autores principales:	Giuseppe D’Amico Ricci, Giovanni Garippa, Stefano Cortese, Rita Serra, Francesco Boscia, Stefano Dore, Antonio Pinna
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f81e6b45e8f4733ba111bc67c0db81f
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spelling	oai:doaj.org-article:6f81e6b45e8f4733ba111bc67c0db81f2021-11-04T06:19:42ZIn vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis1932-6203https://doaj.org/article/6f81e6b45e8f4733ba111bc67c0db81f2021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8547687/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Purpose</h4> To assess the in vitro larvicidal activity of ivermectin and povidone-iodine (PVP-I) against Oestrus ovis, the most frequent cause of external ophthalmomyiasis. <h4>Methods</h4> L1 O. ovis larvae were collected from the nasal boots of sheep slaughtered in local abattoirs and transferred onto Petri dishes containing mucosal tissue (25 larvae/dish). The larvicidal activity of the following formulations was tested: 1% ivermectin suspension in balanced sterile saline solution (BSSS), 1% ivermectin solution in propylene glycol, propylene glycol, 0.6% PVP-I in hyaluronic acid vehicle (IODIM®), and combination of ivermectin 1% solution and 0.6% PVP-I. One mL of each formulation was added to different Petri dishes containing the larvae. The time needed to kill the larvae was recorded. <h4>Results</h4> 893 larvae were tested. The median time needed to kill the larvae was 46, 44, 11, 6, and 10 minutes for Iodim®, ivermectin 1% suspension, propylene glycol, ivermectin 1% solution, and a combination of ivermectin 1% solution with 0.6% PVP-I, respectively. Kaplan-Meyer analysis disclosed that the survival curves were significantly lower in samples treated with ivermectin 1% solution, ivermectin 1% solution + 0.6% PVP-I, and propylene glycol than in samples receiving other treatments or BSSS. <h4>Conclusion</h4> In this in vitro study, ivermectin 1% solution in propylene glycol, ivermectin 1% solution + 0.6% PVP-I, and propylene glycol alone showed a good, relatively rapid larvicidal activity against O. ovis larvae. Further experimental and clinical studies are necessary to establish whether, or not, these formulations may be considered as potential candidates for the topical treatment for external ophthalmomyiasis caused by O. ovis.Giuseppe D’Amico RicciGiovanni GarippaStefano CorteseRita SerraFrancesco BosciaStefano DoreAntonio PinnaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giuseppe D’Amico Ricci Giovanni Garippa Stefano Cortese Rita Serra Francesco Boscia Stefano Dore Antonio Pinna In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis
description	<h4>Purpose</h4> To assess the in vitro larvicidal activity of ivermectin and povidone-iodine (PVP-I) against Oestrus ovis, the most frequent cause of external ophthalmomyiasis. <h4>Methods</h4> L1 O. ovis larvae were collected from the nasal boots of sheep slaughtered in local abattoirs and transferred onto Petri dishes containing mucosal tissue (25 larvae/dish). The larvicidal activity of the following formulations was tested: 1% ivermectin suspension in balanced sterile saline solution (BSSS), 1% ivermectin solution in propylene glycol, propylene glycol, 0.6% PVP-I in hyaluronic acid vehicle (IODIM®), and combination of ivermectin 1% solution and 0.6% PVP-I. One mL of each formulation was added to different Petri dishes containing the larvae. The time needed to kill the larvae was recorded. <h4>Results</h4> 893 larvae were tested. The median time needed to kill the larvae was 46, 44, 11, 6, and 10 minutes for Iodim®, ivermectin 1% suspension, propylene glycol, ivermectin 1% solution, and a combination of ivermectin 1% solution with 0.6% PVP-I, respectively. Kaplan-Meyer analysis disclosed that the survival curves were significantly lower in samples treated with ivermectin 1% solution, ivermectin 1% solution + 0.6% PVP-I, and propylene glycol than in samples receiving other treatments or BSSS. <h4>Conclusion</h4> In this in vitro study, ivermectin 1% solution in propylene glycol, ivermectin 1% solution + 0.6% PVP-I, and propylene glycol alone showed a good, relatively rapid larvicidal activity against O. ovis larvae. Further experimental and clinical studies are necessary to establish whether, or not, these formulations may be considered as potential candidates for the topical treatment for external ophthalmomyiasis caused by O. ovis.
format	article
author	Giuseppe D’Amico Ricci Giovanni Garippa Stefano Cortese Rita Serra Francesco Boscia Stefano Dore Antonio Pinna
author_facet	Giuseppe D’Amico Ricci Giovanni Garippa Stefano Cortese Rita Serra Francesco Boscia Stefano Dore Antonio Pinna
author_sort	Giuseppe D’Amico Ricci
title	In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis
title_short	In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis
title_full	In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis
title_fullStr	In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis
title_full_unstemmed	In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis
title_sort	in vitro larvicidal activity of ivermectin and povidone-iodine against oestrus ovis
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6f81e6b45e8f4733ba111bc67c0db81f
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In vitro larvicidal activity of ivermectin and povidone-iodine against Oestrus ovis

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