Heat Stress in <i>Pinus halepensis</i> Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes

In the current context of climate change, plants need to develop different mechanisms of stress tolerance and adaptation to cope with changing environmental conditions. Temperature is one of the most important abiotic stresses that forest trees have to overcome. Recent research developed in our labo...

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Autores principales:	Cátia Pereira, Ander Castander-Olarieta, Ester Sales, Itziar A. Montalbán, Jorge Canhoto, Paloma Moncaleán
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Aleppo pine conifers <i>DEHYDRATION INDUCED PROTEIN 19</i> epigenetics priming <i>SUPEROXIDE DISMUTASE</i> [<i>Cu–Zn</i>] Botany QK1-989
Acceso en línea:	https://doaj.org/article/6f84c92a0e3b445dac7a9138f443862d
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spelling	oai:doaj.org-article:6f84c92a0e3b445dac7a9138f443862d2021-11-25T18:45:27ZHeat Stress in <i>Pinus halepensis</i> Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes10.3390/plants101123332223-7747https://doaj.org/article/6f84c92a0e3b445dac7a9138f443862d2021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2333https://doaj.org/toc/2223-7747In the current context of climate change, plants need to develop different mechanisms of stress tolerance and adaptation to cope with changing environmental conditions. Temperature is one of the most important abiotic stresses that forest trees have to overcome. Recent research developed in our laboratory demonstrated that high temperatures during different stages of conifer somatic embryogenesis (SE) modify subsequent phases of the process and the behavior of the resulting ex vitro somatic plants. For this reason, Aleppo pine SE was induced under different heat stress treatments (40 °C for 4 h, 50 °C for 30 min, and 60 °C for 5 min) in order to analyze its effect on the global DNA methylation rates and the differential expression of four stress-related genes at different stages of the SE process. Results showed that a slight decrease of DNA methylation at proliferating embryonal masses (EMs) can correlate with the final efficiency of the process. Additionally, different expression patterns for stress-related genes were found in EMs and needles from the in vitro somatic plants obtained; the <i>DEHYDRATION INDUCED PROTEIN 19</i> gene was up-regulated in response to heat at proliferating EMs, whereas <i>HSP20 FAMILY PROTEIN</i> and <i>SUPEROXIDE DISMUTASE</i> [<i>Cu–Zn</i>] were down-regulated in needles.Cátia PereiraAnder Castander-OlarietaEster SalesItziar A. MontalbánJorge CanhotoPaloma MoncaleánMDPI AGarticleAleppo pineconifers<i>DEHYDRATION INDUCED PROTEIN 19</i>epigeneticspriming<i>SUPEROXIDE DISMUTASE</i> [<i>Cu–Zn</i>]BotanyQK1-989ENPlants, Vol 10, Iss 2333, p 2333 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aleppo pine conifers <i>DEHYDRATION INDUCED PROTEIN 19</i> epigenetics priming <i>SUPEROXIDE DISMUTASE</i> [<i>Cu–Zn</i>] Botany QK1-989
spellingShingle	Aleppo pine conifers <i>DEHYDRATION INDUCED PROTEIN 19</i> epigenetics priming <i>SUPEROXIDE DISMUTASE</i> [<i>Cu–Zn</i>] Botany QK1-989 Cátia Pereira Ander Castander-Olarieta Ester Sales Itziar A. Montalbán Jorge Canhoto Paloma Moncaleán Heat Stress in <i>Pinus halepensis</i> Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes
description	In the current context of climate change, plants need to develop different mechanisms of stress tolerance and adaptation to cope with changing environmental conditions. Temperature is one of the most important abiotic stresses that forest trees have to overcome. Recent research developed in our laboratory demonstrated that high temperatures during different stages of conifer somatic embryogenesis (SE) modify subsequent phases of the process and the behavior of the resulting ex vitro somatic plants. For this reason, Aleppo pine SE was induced under different heat stress treatments (40 °C for 4 h, 50 °C for 30 min, and 60 °C for 5 min) in order to analyze its effect on the global DNA methylation rates and the differential expression of four stress-related genes at different stages of the SE process. Results showed that a slight decrease of DNA methylation at proliferating embryonal masses (EMs) can correlate with the final efficiency of the process. Additionally, different expression patterns for stress-related genes were found in EMs and needles from the in vitro somatic plants obtained; the <i>DEHYDRATION INDUCED PROTEIN 19</i> gene was up-regulated in response to heat at proliferating EMs, whereas <i>HSP20 FAMILY PROTEIN</i> and <i>SUPEROXIDE DISMUTASE</i> [<i>Cu–Zn</i>] were down-regulated in needles.
format	article
author	Cátia Pereira Ander Castander-Olarieta Ester Sales Itziar A. Montalbán Jorge Canhoto Paloma Moncaleán
author_facet	Cátia Pereira Ander Castander-Olarieta Ester Sales Itziar A. Montalbán Jorge Canhoto Paloma Moncaleán
author_sort	Cátia Pereira
title	Heat Stress in <i>Pinus halepensis</i> Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes
title_short	Heat Stress in <i>Pinus halepensis</i> Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes
title_full	Heat Stress in <i>Pinus halepensis</i> Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes
title_fullStr	Heat Stress in <i>Pinus halepensis</i> Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes
title_full_unstemmed	Heat Stress in <i>Pinus halepensis</i> Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes
title_sort	heat stress in <i>pinus halepensis</i> somatic embryogenesis induction: effect in dna methylation and differential expression of stress-related genes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6f84c92a0e3b445dac7a9138f443862d
work_keys_str_mv	AT catiapereira heatstressinipinushalepensisisomaticembryogenesisinductioneffectindnamethylationanddifferentialexpressionofstressrelatedgenes AT andercastanderolarieta heatstressinipinushalepensisisomaticembryogenesisinductioneffectindnamethylationanddifferentialexpressionofstressrelatedgenes AT estersales heatstressinipinushalepensisisomaticembryogenesisinductioneffectindnamethylationanddifferentialexpressionofstressrelatedgenes AT itziaramontalban heatstressinipinushalepensisisomaticembryogenesisinductioneffectindnamethylationanddifferentialexpressionofstressrelatedgenes AT jorgecanhoto heatstressinipinushalepensisisomaticembryogenesisinductioneffectindnamethylationanddifferentialexpressionofstressrelatedgenes AT palomamoncalean heatstressinipinushalepensisisomaticembryogenesisinductioneffectindnamethylationanddifferentialexpressionofstressrelatedgenes
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Heat Stress in <i>Pinus halepensis</i> Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes

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