Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria

Syed Zeeshan Haider Naqvi, Urooj Kiran, Muhammad Ishtiaq Ali, Asif Jamal, Abdul Hameed, Safia Ahmed, Naeem Ali Microbiology Research Laboratory, Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan Abstract: Biological synthesis of nanoparticles is...

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Autores principales:	Naqvi SZ, Kiran U, Ali MI, Jamal A, Hameed A, Ahmed S, Ali N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6f854990206f4a7fb9ae6dfc276551e0
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spelling	oai:doaj.org-article:6f854990206f4a7fb9ae6dfc276551e02021-12-02T01:43:42ZCombined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria1176-91141178-2013https://doaj.org/article/6f854990206f4a7fb9ae6dfc276551e02013-08-01T00:00:00Zhttp://www.dovepress.com/combined-efficacy-of-biologically-synthesized-silver-nanoparticles-and-a14089https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Syed Zeeshan Haider Naqvi, Urooj Kiran, Muhammad Ishtiaq Ali, Asif Jamal, Abdul Hameed, Safia Ahmed, Naeem Ali Microbiology Research Laboratory, Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan Abstract: Biological synthesis of nanoparticles is a growing innovative approach that is relatively cheaper and more environmentally friendly than current physicochemical processes. Among various microorganisms, fungi have been found to be comparatively more efficient in the synthesis of nanomaterials. In this research work, extracellular mycosynthesis of silver nanoparticles (AgNPs) was probed by reacting the precursor salt of silver nitrate (AgNO3) with culture filtrate of Aspergillus flavus. Initially, the mycosynthesis was regularly monitored by ultraviolet-visible spectroscopy, which showed AgNP peaks of around 400–470 nm. X-ray diffraction spectra revealed peaks of different intensities with respect to angle of diffractions (2θ) corresponding to varying configurations of AgNPs. Transmission electron micrographs further confirmed the formation of AgNPs in size ranging from 5–30 nm. Combined and individual antibacterial activities of the five conventional antibiotics and AgNPs were investigated against eight different multidrug-resistant bacterial species using the Kirby–Bauer disk-diffusion method. The decreasing order of antibacterial activity (zone of inhibition in mm) of antibiotics, AgNPs, and their conjugates against bacterial group (average) was; ciprofloxacin + AgNPs (23) > imipenem + AgNPs (21) > gentamycin + AgNPs (19) > vancomycin + AgNPs (16) > AgNPs (15) > imipenem (14) > trimethoprim + AgNPs (14) > ciprofloxacin (13) > gentamycin (11) > vancomycin (4) > trimethoprim (0). Overall, the synergistic effect of antibiotics and nanoparticles resulted in a 0.2–7.0 (average, 2.8) fold-area increase in antibacterial activity, which clearly revealed that nanoparticles can be effectively used in combination with antibiotics in order to improve their efficacy against various pathogenic microbes. Keywords: mycosynthesis, silver nanoparticles, antibacterial agents, Aspergillus flavusNaqvi SZKiran UAli MIJamal AHameed AAhmed SAli NDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 3187-3195 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Naqvi SZ Kiran U Ali MI Jamal A Hameed A Ahmed S Ali N Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria
description	Syed Zeeshan Haider Naqvi, Urooj Kiran, Muhammad Ishtiaq Ali, Asif Jamal, Abdul Hameed, Safia Ahmed, Naeem Ali Microbiology Research Laboratory, Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan Abstract: Biological synthesis of nanoparticles is a growing innovative approach that is relatively cheaper and more environmentally friendly than current physicochemical processes. Among various microorganisms, fungi have been found to be comparatively more efficient in the synthesis of nanomaterials. In this research work, extracellular mycosynthesis of silver nanoparticles (AgNPs) was probed by reacting the precursor salt of silver nitrate (AgNO3) with culture filtrate of Aspergillus flavus. Initially, the mycosynthesis was regularly monitored by ultraviolet-visible spectroscopy, which showed AgNP peaks of around 400–470 nm. X-ray diffraction spectra revealed peaks of different intensities with respect to angle of diffractions (2θ) corresponding to varying configurations of AgNPs. Transmission electron micrographs further confirmed the formation of AgNPs in size ranging from 5–30 nm. Combined and individual antibacterial activities of the five conventional antibiotics and AgNPs were investigated against eight different multidrug-resistant bacterial species using the Kirby–Bauer disk-diffusion method. The decreasing order of antibacterial activity (zone of inhibition in mm) of antibiotics, AgNPs, and their conjugates against bacterial group (average) was; ciprofloxacin + AgNPs (23) > imipenem + AgNPs (21) > gentamycin + AgNPs (19) > vancomycin + AgNPs (16) > AgNPs (15) > imipenem (14) > trimethoprim + AgNPs (14) > ciprofloxacin (13) > gentamycin (11) > vancomycin (4) > trimethoprim (0). Overall, the synergistic effect of antibiotics and nanoparticles resulted in a 0.2–7.0 (average, 2.8) fold-area increase in antibacterial activity, which clearly revealed that nanoparticles can be effectively used in combination with antibiotics in order to improve their efficacy against various pathogenic microbes. Keywords: mycosynthesis, silver nanoparticles, antibacterial agents, Aspergillus flavus
format	article
author	Naqvi SZ Kiran U Ali MI Jamal A Hameed A Ahmed S Ali N
author_facet	Naqvi SZ Kiran U Ali MI Jamal A Hameed A Ahmed S Ali N
author_sort	Naqvi SZ
title	Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria
title_short	Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria
title_full	Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria
title_fullStr	Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria
title_full_unstemmed	Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria
title_sort	combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/6f854990206f4a7fb9ae6dfc276551e0
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Combined efficacy of biologically synthesized silver nanoparticles and different antibiotics against multidrug-resistant bacteria

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