Effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers

Qing Qing Dou,1 Choon Peng Teng,1 Enyi Ye,1 Xian Jun Loh1–3 1Institute of Materials Research and Engineering, A*STAR, Singapore; 2Department of Materials Science and Engineering, National University of Singapore, Singapore; 3Singapore Eye Research Institute, Singapore Abstract:...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dou QQ, Teng CP, Ye E, Loh XJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6f9427fd25c344b1839d7b92518c7fcc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6f9427fd25c344b1839d7b92518c7fcc
record_format	dspace
spelling	oai:doaj.org-article:6f9427fd25c344b1839d7b92518c7fcc2021-12-02T06:31:47ZEffective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers1178-2013https://doaj.org/article/6f9427fd25c344b1839d7b92518c7fcc2015-01-01T00:00:00Zhttp://www.dovepress.com/effective-near-infrared-photodynamic-therapy-assisted-by-upconversion--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Qing Qing Dou,1 Choon Peng Teng,1 Enyi Ye,1 Xian Jun Loh1–3 1Institute of Materials Research and Engineering, A*STAR, Singapore; 2Department of Materials Science and Engineering, National University of Singapore, Singapore; 3Singapore Eye Research Institute, Singapore Abstract: A drug model photosensitizer–conjugated upconversion nanoparticles nanocomplex was explored for application in near-infrared photodynamic therapy. As near-infrared penetrates deeper into the tissue, the model is useful for the application of photodynamic therapy in deeper tissue. The nanocomplex that was synthesized had low polydispersity, and the upconversion nanoparticle was covalently conjugated with the photosensitizer. The robust bond could prevent the undesired premature release of photosensitizer and also enhance the singlet-oxygen generation. Singlet-oxygen generation rate from this nanocomplex was evaluated in solution. The photodynamic therapy effect was assessed with MCF-7 cells in two different methods, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and live/dead assay. The assay results showed that promising efficacy (>90%) can be achieved with a low concentration (50 µg mL-1) of this nanocomplex and mild dosage (7 mW cm-2) of near-infrared laser treatment. Keywords: nanocomplex, singlet-oxygen generation rate, MTT assay, live/dead assayDou QQTeng CPYe ELoh XJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 419-432 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Dou QQ Teng CP Ye E Loh XJ Effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers
description	Qing Qing Dou,1 Choon Peng Teng,1 Enyi Ye,1 Xian Jun Loh1–3 1Institute of Materials Research and Engineering, A*STAR, Singapore; 2Department of Materials Science and Engineering, National University of Singapore, Singapore; 3Singapore Eye Research Institute, Singapore Abstract: A drug model photosensitizer–conjugated upconversion nanoparticles nanocomplex was explored for application in near-infrared photodynamic therapy. As near-infrared penetrates deeper into the tissue, the model is useful for the application of photodynamic therapy in deeper tissue. The nanocomplex that was synthesized had low polydispersity, and the upconversion nanoparticle was covalently conjugated with the photosensitizer. The robust bond could prevent the undesired premature release of photosensitizer and also enhance the singlet-oxygen generation. Singlet-oxygen generation rate from this nanocomplex was evaluated in solution. The photodynamic therapy effect was assessed with MCF-7 cells in two different methods, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and live/dead assay. The assay results showed that promising efficacy (>90%) can be achieved with a low concentration (50 µg mL-1) of this nanocomplex and mild dosage (7 mW cm-2) of near-infrared laser treatment. Keywords: nanocomplex, singlet-oxygen generation rate, MTT assay, live/dead assay
format	article
author	Dou QQ Teng CP Ye E Loh XJ
author_facet	Dou QQ Teng CP Ye E Loh XJ
author_sort	Dou QQ
title	Effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers
title_short	Effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers
title_full	Effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers
title_fullStr	Effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers
title_full_unstemmed	Effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers
title_sort	effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/6f9427fd25c344b1839d7b92518c7fcc
work_keys_str_mv	AT douqq effectivenearinfraredphotodynamictherapyassistedbyupconversionnanoparticlesconjugatedwithphotosensitizers AT tengcp effectivenearinfraredphotodynamictherapyassistedbyupconversionnanoparticlesconjugatedwithphotosensitizers AT yee effectivenearinfraredphotodynamictherapyassistedbyupconversionnanoparticlesconjugatedwithphotosensitizers AT lohxj effectivenearinfraredphotodynamictherapyassistedbyupconversionnanoparticlesconjugatedwithphotosensitizers
_version_	1718399876351393792

Effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers

Ejemplares similares