Bioactive Collagen Hydrolysate-Chitosan/Essential Oil Electrospun Nanofibers Designed for Medical Wound Dressings

In this study, lemon balm (<i>Melissa officinalis</i> L.) and dill (<i>Anethum graveolens</i> L.) essential oils (EOs) were encapsulated into collagen hydrolysates extracted from bovine tendons and rabbit skins, both mixed with chitosan (CS) by using the coaxial electrospinni...

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Autores principales:	Maria Râpă, Carmen Gaidau, Liliana Mititelu-Tartau, Mariana-Daniela Berechet, Andrei Constantin Berbecaru, Irina Rosca, Aurica P. Chiriac, Ecaterina Matei, Andra-Mihaela Predescu, Cristian Predescu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	wound dressing collagen hydrolysate and chitosan lemon balm essential oil dill essential oil in vivo biocompatibility antimicrobial activity Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/6fa04216681f4d2ea69afb6261cd4ab5
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spelling	oai:doaj.org-article:6fa04216681f4d2ea69afb6261cd4ab52021-11-25T18:42:11ZBioactive Collagen Hydrolysate-Chitosan/Essential Oil Electrospun Nanofibers Designed for Medical Wound Dressings10.3390/pharmaceutics131119391999-4923https://doaj.org/article/6fa04216681f4d2ea69afb6261cd4ab52021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1939https://doaj.org/toc/1999-4923In this study, lemon balm (<i>Melissa officinalis</i> L.) and dill (<i>Anethum graveolens</i> L.) essential oils (EOs) were encapsulated into collagen hydrolysates extracted from bovine tendons and rabbit skins, both mixed with chitosan (CS) by using the coaxial electrospinning technique for potential wound dressing applications. The morphology and chemical composition of the electrospun nanofibers were investigated using scanning electron microscopy (SEM) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The antimicrobial activity of the dill EO and lemon EO, as well as the electrospun samples loaded with essential oils was determined by disk diffusion assay against <i>Staphylococcus aureus</i> ATCC 25923, <i>Escherichia coli</i> ATCC 25922, <i>Enterococcus faecalis</i> ATCC 29212, and <i>Salmonella typhimurium</i> ATCC 14028 bacterial strains; <i>Candida albicans</i> ATCC 10231 and <i>Candida glabrata</i> ATCC 90028 yeast strains; and <i>Aspergillus brasiliensis</i> ATCC 9642 fungal strain. In vivo biocompatibility testing of the collagen hydrolysate-chitosan/essential oil electrospun nanofibers was based on the determination of the hematological, biochemical, and immunological profile and the evaluation of the influence produced on the oxidative stress in white Swiss mice. The synergetic effect of dill and lemon balm EOs can improve the antimicrobial activity of collagen hydrolysate-chitosan nanofibers against the most important bacterial strains. The in vivo test results suggested a good biocompatibility of electrospun samples based on collagen hydrolysate extracted from bovine tendons or rabbit skin mixed with chitosan and containing dill and/or lemon balm essential oils as encapsulated bioactive compounds.Maria RâpăCarmen GaidauLiliana Mititelu-TartauMariana-Daniela BerechetAndrei Constantin BerbecaruIrina RoscaAurica P. ChiriacEcaterina MateiAndra-Mihaela PredescuCristian PredescuMDPI AGarticlewound dressingcollagen hydrolysate and chitosanlemon balm essential oildill essential oilin vivo biocompatibilityantimicrobial activityPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1939, p 1939 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	wound dressing collagen hydrolysate and chitosan lemon balm essential oil dill essential oil in vivo biocompatibility antimicrobial activity Pharmacy and materia medica RS1-441
spellingShingle	wound dressing collagen hydrolysate and chitosan lemon balm essential oil dill essential oil in vivo biocompatibility antimicrobial activity Pharmacy and materia medica RS1-441 Maria Râpă Carmen Gaidau Liliana Mititelu-Tartau Mariana-Daniela Berechet Andrei Constantin Berbecaru Irina Rosca Aurica P. Chiriac Ecaterina Matei Andra-Mihaela Predescu Cristian Predescu Bioactive Collagen Hydrolysate-Chitosan/Essential Oil Electrospun Nanofibers Designed for Medical Wound Dressings
description	In this study, lemon balm (<i>Melissa officinalis</i> L.) and dill (<i>Anethum graveolens</i> L.) essential oils (EOs) were encapsulated into collagen hydrolysates extracted from bovine tendons and rabbit skins, both mixed with chitosan (CS) by using the coaxial electrospinning technique for potential wound dressing applications. The morphology and chemical composition of the electrospun nanofibers were investigated using scanning electron microscopy (SEM) and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The antimicrobial activity of the dill EO and lemon EO, as well as the electrospun samples loaded with essential oils was determined by disk diffusion assay against <i>Staphylococcus aureus</i> ATCC 25923, <i>Escherichia coli</i> ATCC 25922, <i>Enterococcus faecalis</i> ATCC 29212, and <i>Salmonella typhimurium</i> ATCC 14028 bacterial strains; <i>Candida albicans</i> ATCC 10231 and <i>Candida glabrata</i> ATCC 90028 yeast strains; and <i>Aspergillus brasiliensis</i> ATCC 9642 fungal strain. In vivo biocompatibility testing of the collagen hydrolysate-chitosan/essential oil electrospun nanofibers was based on the determination of the hematological, biochemical, and immunological profile and the evaluation of the influence produced on the oxidative stress in white Swiss mice. The synergetic effect of dill and lemon balm EOs can improve the antimicrobial activity of collagen hydrolysate-chitosan nanofibers against the most important bacterial strains. The in vivo test results suggested a good biocompatibility of electrospun samples based on collagen hydrolysate extracted from bovine tendons or rabbit skin mixed with chitosan and containing dill and/or lemon balm essential oils as encapsulated bioactive compounds.
format	article
author	Maria Râpă Carmen Gaidau Liliana Mititelu-Tartau Mariana-Daniela Berechet Andrei Constantin Berbecaru Irina Rosca Aurica P. Chiriac Ecaterina Matei Andra-Mihaela Predescu Cristian Predescu
author_facet	Maria Râpă Carmen Gaidau Liliana Mititelu-Tartau Mariana-Daniela Berechet Andrei Constantin Berbecaru Irina Rosca Aurica P. Chiriac Ecaterina Matei Andra-Mihaela Predescu Cristian Predescu
author_sort	Maria Râpă
title	Bioactive Collagen Hydrolysate-Chitosan/Essential Oil Electrospun Nanofibers Designed for Medical Wound Dressings
title_short	Bioactive Collagen Hydrolysate-Chitosan/Essential Oil Electrospun Nanofibers Designed for Medical Wound Dressings
title_full	Bioactive Collagen Hydrolysate-Chitosan/Essential Oil Electrospun Nanofibers Designed for Medical Wound Dressings
title_fullStr	Bioactive Collagen Hydrolysate-Chitosan/Essential Oil Electrospun Nanofibers Designed for Medical Wound Dressings
title_full_unstemmed	Bioactive Collagen Hydrolysate-Chitosan/Essential Oil Electrospun Nanofibers Designed for Medical Wound Dressings
title_sort	bioactive collagen hydrolysate-chitosan/essential oil electrospun nanofibers designed for medical wound dressings
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6fa04216681f4d2ea69afb6261cd4ab5
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Bioactive Collagen Hydrolysate-Chitosan/Essential Oil Electrospun Nanofibers Designed for Medical Wound Dressings

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