Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry.

Assays for the detection of the iron regulatory hormone hepcidin in plasma or urine have not yet been widely available, whereas quantitative comparisons between hepcidin levels in these different matrices were thus far even impossible due to technical restrictions. To circumvent these limitations, w...

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Autores principales:	Dorine W Swinkels, Domenico Girelli, Coby Laarakkers, Joyce Kroot, Natascia Campostrini, Erwin H J M Kemna, Harold Tjalsma
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6fa15829fd154dcca99d54852dce3763
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spelling	oai:doaj.org-article:6fa15829fd154dcca99d54852dce37632021-11-25T06:11:34ZAdvances in quantitative hepcidin measurements by time-of-flight mass spectrometry.1932-620310.1371/journal.pone.0002706https://doaj.org/article/6fa15829fd154dcca99d54852dce37632008-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18628991/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Assays for the detection of the iron regulatory hormone hepcidin in plasma or urine have not yet been widely available, whereas quantitative comparisons between hepcidin levels in these different matrices were thus far even impossible due to technical restrictions. To circumvent these limitations, we here describe several advances in time-of flight mass spectrometry (TOF MS), the most important of which concerned spiking of a synthetic hepcidin analogue as internal standard into serum and urine samples. This serves both as a control for experimental variation, such as recovery and matrix-dependent ionization and ion suppression, and at the same time allows value assignment to the measured hepcidin peak intensities. The assay improvements were clinically evaluated using samples from various patients groups and its relevance was further underscored by the significant correlation of serum hepcidin levels with serum iron indices in healthy individuals. Most importantly, this approach allowed kinetic studies as illustrated by the paired analyses of serum and urine samples, showing that more than 97% of the freely filtered serum hepcidin can be reabsorbed in the kidney. Thus, the here reported advances in TOF MS-based hepcidin measurements represent critical steps in the accurate quantification of hepcidin in various body fluids and pave the way for clinical studies on the kinetic behavior of hepcidin in both healthy and diseased states.Dorine W SwinkelsDomenico GirelliCoby LaarakkersJoyce KrootNatascia CampostriniErwin H J M KemnaHarold TjalsmaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 7, p e2706 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dorine W Swinkels Domenico Girelli Coby Laarakkers Joyce Kroot Natascia Campostrini Erwin H J M Kemna Harold Tjalsma Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry.
description	Assays for the detection of the iron regulatory hormone hepcidin in plasma or urine have not yet been widely available, whereas quantitative comparisons between hepcidin levels in these different matrices were thus far even impossible due to technical restrictions. To circumvent these limitations, we here describe several advances in time-of flight mass spectrometry (TOF MS), the most important of which concerned spiking of a synthetic hepcidin analogue as internal standard into serum and urine samples. This serves both as a control for experimental variation, such as recovery and matrix-dependent ionization and ion suppression, and at the same time allows value assignment to the measured hepcidin peak intensities. The assay improvements were clinically evaluated using samples from various patients groups and its relevance was further underscored by the significant correlation of serum hepcidin levels with serum iron indices in healthy individuals. Most importantly, this approach allowed kinetic studies as illustrated by the paired analyses of serum and urine samples, showing that more than 97% of the freely filtered serum hepcidin can be reabsorbed in the kidney. Thus, the here reported advances in TOF MS-based hepcidin measurements represent critical steps in the accurate quantification of hepcidin in various body fluids and pave the way for clinical studies on the kinetic behavior of hepcidin in both healthy and diseased states.
format	article
author	Dorine W Swinkels Domenico Girelli Coby Laarakkers Joyce Kroot Natascia Campostrini Erwin H J M Kemna Harold Tjalsma
author_facet	Dorine W Swinkels Domenico Girelli Coby Laarakkers Joyce Kroot Natascia Campostrini Erwin H J M Kemna Harold Tjalsma
author_sort	Dorine W Swinkels
title	Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry.
title_short	Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry.
title_full	Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry.
title_fullStr	Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry.
title_full_unstemmed	Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry.
title_sort	advances in quantitative hepcidin measurements by time-of-flight mass spectrometry.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/6fa15829fd154dcca99d54852dce3763
work_keys_str_mv	AT dorinewswinkels advancesinquantitativehepcidinmeasurementsbytimeofflightmassspectrometry AT domenicogirelli advancesinquantitativehepcidinmeasurementsbytimeofflightmassspectrometry AT cobylaarakkers advancesinquantitativehepcidinmeasurementsbytimeofflightmassspectrometry AT joycekroot advancesinquantitativehepcidinmeasurementsbytimeofflightmassspectrometry AT natasciacampostrini advancesinquantitativehepcidinmeasurementsbytimeofflightmassspectrometry AT erwinhjmkemna advancesinquantitativehepcidinmeasurementsbytimeofflightmassspectrometry AT haroldtjalsma advancesinquantitativehepcidinmeasurementsbytimeofflightmassspectrometry
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Advances in quantitative hepcidin measurements by time-of-flight mass spectrometry.

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