Metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study

Abstract Background Periapical periodontitis is a common oral inflammatory disease that affects periapical tissues and is caused by bacteria in the root canal system. The relationship among the local metabolome, the inflammatory grade, and the type and abundance of microorganisms associated with per...

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Autores principales:	Yun Huang, Peng Zhou, Siqi Liu, Wei Duan, Qinqin Zhang, Ying Lu, Xin Wei
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Periapical periodontitis Inflammatory grades Metabolome Microbiome Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/6fa1cb5b99534802bf3804f69c58bfe2
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spelling	oai:doaj.org-article:6fa1cb5b99534802bf3804f69c58bfe22021-11-28T12:29:26ZMetabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study10.1186/s12903-021-01972-81472-6831https://doaj.org/article/6fa1cb5b99534802bf3804f69c58bfe22021-11-01T00:00:00Zhttps://doi.org/10.1186/s12903-021-01972-8https://doaj.org/toc/1472-6831Abstract Background Periapical periodontitis is a common oral inflammatory disease that affects periapical tissues and is caused by bacteria in the root canal system. The relationship among the local metabolome, the inflammatory grade, and the type and abundance of microorganisms associated with periapical periodontitis is discussed in this study. Methods The inflammatory grades of periapical samples from 47 patients with chronic periapical periodontitis in permanent anterior teeth were determined based on the immune cell densities in tissues subjected to haematoxylin and eosin staining. The metabolome was evaluated using ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, followed by principal component analysis and orthogonal partial least squares discriminant analysis. The microbiome was accessed using 16 S rRNA high-throughput sequencing. The differences in the metabolomes and microbiomes of the periapical periodontitis samples were assessed using Spearman’s correlation analysis. Result N-acetyl-D-glucosamine, L-tryptophan, L-phenylalanine, and 15 other metabolites were identified by the comparison between samples with severe inflammation and mild or moderate inflammation. Four amino acid metabolism pathways and one sugar metabolism pathway were associated with the inflammatory grade of periapical periodontitis. The abundance of Actinomycetes was negatively correlated with the abundance of glucosamine (GlcN), while the abundance of Tannerella was positively correlated with the abundance of L-methionine. Conclusions The local metabolome of periapical periodontitis is correlated with the inflammatory grade. The abundance of the local metabolites GlcN and L-methionine is correlated with the abundance of the major microorganisms Actinomycetes and Tannerella, respectively.Yun HuangPeng ZhouSiqi LiuWei DuanQinqin ZhangYing LuXin WeiBMCarticlePeriapical periodontitisInflammatory gradesMetabolomeMicrobiomeDentistryRK1-715ENBMC Oral Health, Vol 21, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Periapical periodontitis Inflammatory grades Metabolome Microbiome Dentistry RK1-715
spellingShingle	Periapical periodontitis Inflammatory grades Metabolome Microbiome Dentistry RK1-715 Yun Huang Peng Zhou Siqi Liu Wei Duan Qinqin Zhang Ying Lu Xin Wei Metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study
description	Abstract Background Periapical periodontitis is a common oral inflammatory disease that affects periapical tissues and is caused by bacteria in the root canal system. The relationship among the local metabolome, the inflammatory grade, and the type and abundance of microorganisms associated with periapical periodontitis is discussed in this study. Methods The inflammatory grades of periapical samples from 47 patients with chronic periapical periodontitis in permanent anterior teeth were determined based on the immune cell densities in tissues subjected to haematoxylin and eosin staining. The metabolome was evaluated using ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, followed by principal component analysis and orthogonal partial least squares discriminant analysis. The microbiome was accessed using 16 S rRNA high-throughput sequencing. The differences in the metabolomes and microbiomes of the periapical periodontitis samples were assessed using Spearman’s correlation analysis. Result N-acetyl-D-glucosamine, L-tryptophan, L-phenylalanine, and 15 other metabolites were identified by the comparison between samples with severe inflammation and mild or moderate inflammation. Four amino acid metabolism pathways and one sugar metabolism pathway were associated with the inflammatory grade of periapical periodontitis. The abundance of Actinomycetes was negatively correlated with the abundance of glucosamine (GlcN), while the abundance of Tannerella was positively correlated with the abundance of L-methionine. Conclusions The local metabolome of periapical periodontitis is correlated with the inflammatory grade. The abundance of the local metabolites GlcN and L-methionine is correlated with the abundance of the major microorganisms Actinomycetes and Tannerella, respectively.
format	article
author	Yun Huang Peng Zhou Siqi Liu Wei Duan Qinqin Zhang Ying Lu Xin Wei
author_facet	Yun Huang Peng Zhou Siqi Liu Wei Duan Qinqin Zhang Ying Lu Xin Wei
author_sort	Yun Huang
title	Metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study
title_short	Metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study
title_full	Metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study
title_fullStr	Metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study
title_full_unstemmed	Metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study
title_sort	metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study
publisher	BMC
publishDate	2021
url	https://doaj.org/article/6fa1cb5b99534802bf3804f69c58bfe2
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_version_	1718407961689194496

Metabolome and microbiome of chronic periapical periodontitis in permanent anterior teeth: a pilot study

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