Cytotoxic Effect of Titanium Dioxide-Hydroxyurea Nanocamposit on Hela Cancer Cell Line

BACKGROUND AND OBJECTIVE: The use of nanotechnology in drug delivery Not only increase the efficacy and ease of drug penetration, but also they decrease their adverse effects. In this study, TiO2 nanoparticle was used as Hydroxyl Urea carrier to increase the contact surface of the drug and cells, an...

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Autores principales:	R Ghahremani Farasfaji, M Bikhof Torbati, F Tafvizi, M Shaabanzadeh
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2019
Materias:	hydroxy urea titanium hela cells anti-cancer drugs apoptosis Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6fa3c4c4ae6d4693933993c775211a1c
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spelling	oai:doaj.org-article:6fa3c4c4ae6d4693933993c775211a1c2021-11-10T08:30:36ZCytotoxic Effect of Titanium Dioxide-Hydroxyurea Nanocamposit on Hela Cancer Cell Line1561-41072251-7170https://doaj.org/article/6fa3c4c4ae6d4693933993c775211a1c2019-12-01T00:00:00Zhttp://jbums.org/article-1-8084-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: The use of nanotechnology in drug delivery Not only increase the efficacy and ease of drug penetration, but also they decrease their adverse effects. In this study, TiO2 nanoparticle was used as Hydroxyl Urea carrier to increase the contact surface of the drug and cells, and with pegylation of nanoparticle surface decrease immunogenicity, hence to increase drug solubility and penetration to cells. The goal of this study was to investigate cytotoxicity of synthesized TiO2-Poly Ethylene Glycol-Hydroxy Urea (TiO2-PEG-HU) nanocomposite on Hela cell-line and apoptosis induction of treated cells compared to the control group to determine the effective dose of nanodrug. METHODS: In this laboratory study, the effect of TiO2-PEG-HU nano-drug was evaluated on cells bioactivity by MTT method at concentrations of 200, 400, 800, and 1800 µg/ml in 48 and 120 hours. Annexin-V/PI flowcytometry method was used to analyze apoptosis induction. Data were analyzed using uni-directional variance and independent T-test. FINDINGS: Higher concentrations of TiO2-PEG-HU nanocomposite decreased cells bioactivity dependent on dosage and time. As the concentration of 1600 µg/ml of nanocomposite reduced amount of bioavailability by 1.52 times over a 120-hour period compared to the 48-hour time-effect. For both times, this reduced cell survival was `significantly different from that of the control group at the level of p <0.0001. In addition, nano-drug significantly increased apoptosis induction 2.5 times in treated Hela cells (p=0.0114). CONCLUSION: Nano-composite TiO2-PEG-HU on the Hela cell line is cytotoxic and induces apoptosis and can be a promising drug for cancer treatment.R Ghahremani FarasfajiM Bikhof TorbatiF TafviziM ShaabanzadehBabol University of Medical Sciencesarticlehydroxy ureatitaniumhela cellsanti-cancer drugsapoptosisMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 116-121 (2019)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	hydroxy urea titanium hela cells anti-cancer drugs apoptosis Medicine R Medicine (General) R5-920
spellingShingle	hydroxy urea titanium hela cells anti-cancer drugs apoptosis Medicine R Medicine (General) R5-920 R Ghahremani Farasfaji M Bikhof Torbati F Tafvizi M Shaabanzadeh Cytotoxic Effect of Titanium Dioxide-Hydroxyurea Nanocamposit on Hela Cancer Cell Line
description	BACKGROUND AND OBJECTIVE: The use of nanotechnology in drug delivery Not only increase the efficacy and ease of drug penetration, but also they decrease their adverse effects. In this study, TiO2 nanoparticle was used as Hydroxyl Urea carrier to increase the contact surface of the drug and cells, and with pegylation of nanoparticle surface decrease immunogenicity, hence to increase drug solubility and penetration to cells. The goal of this study was to investigate cytotoxicity of synthesized TiO2-Poly Ethylene Glycol-Hydroxy Urea (TiO2-PEG-HU) nanocomposite on Hela cell-line and apoptosis induction of treated cells compared to the control group to determine the effective dose of nanodrug. METHODS: In this laboratory study, the effect of TiO2-PEG-HU nano-drug was evaluated on cells bioactivity by MTT method at concentrations of 200, 400, 800, and 1800 µg/ml in 48 and 120 hours. Annexin-V/PI flowcytometry method was used to analyze apoptosis induction. Data were analyzed using uni-directional variance and independent T-test. FINDINGS: Higher concentrations of TiO2-PEG-HU nanocomposite decreased cells bioactivity dependent on dosage and time. As the concentration of 1600 µg/ml of nanocomposite reduced amount of bioavailability by 1.52 times over a 120-hour period compared to the 48-hour time-effect. For both times, this reduced cell survival was `significantly different from that of the control group at the level of p <0.0001. In addition, nano-drug significantly increased apoptosis induction 2.5 times in treated Hela cells (p=0.0114). CONCLUSION: Nano-composite TiO2-PEG-HU on the Hela cell line is cytotoxic and induces apoptosis and can be a promising drug for cancer treatment.
format	article
author	R Ghahremani Farasfaji M Bikhof Torbati F Tafvizi M Shaabanzadeh
author_facet	R Ghahremani Farasfaji M Bikhof Torbati F Tafvizi M Shaabanzadeh
author_sort	R Ghahremani Farasfaji
title	Cytotoxic Effect of Titanium Dioxide-Hydroxyurea Nanocamposit on Hela Cancer Cell Line
title_short	Cytotoxic Effect of Titanium Dioxide-Hydroxyurea Nanocamposit on Hela Cancer Cell Line
title_full	Cytotoxic Effect of Titanium Dioxide-Hydroxyurea Nanocamposit on Hela Cancer Cell Line
title_fullStr	Cytotoxic Effect of Titanium Dioxide-Hydroxyurea Nanocamposit on Hela Cancer Cell Line
title_full_unstemmed	Cytotoxic Effect of Titanium Dioxide-Hydroxyurea Nanocamposit on Hela Cancer Cell Line
title_sort	cytotoxic effect of titanium dioxide-hydroxyurea nanocamposit on hela cancer cell line
publisher	Babol University of Medical Sciences
publishDate	2019
url	https://doaj.org/article/6fa3c4c4ae6d4693933993c775211a1c
work_keys_str_mv	AT rghahremanifarasfaji cytotoxiceffectoftitaniumdioxidehydroxyureananocampositonhelacancercellline AT mbikhoftorbati cytotoxiceffectoftitaniumdioxidehydroxyureananocampositonhelacancercellline AT ftafvizi cytotoxiceffectoftitaniumdioxidehydroxyureananocampositonhelacancercellline AT mshaabanzadeh cytotoxiceffectoftitaniumdioxidehydroxyureananocampositonhelacancercellline
_version_	1718440384030310400

Cytotoxic Effect of Titanium Dioxide-Hydroxyurea Nanocamposit on Hela Cancer Cell Line

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