Introduction of a microsurgical in-vivo embolization-model in rats: the aorta-filter model.

Vascular thrombosis with subsequent distal embolization remains a critical event for patients. Prevention of this life-threatening event can be achieved pharmacologically or mechanically with intravascular filter systems. The ability to evaluate the risk of embolization of certain techniques and pro...

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Autores principales:	Lucas M Ritschl, Andreas M Fichter, Monika von Düring, David A Mitchell, Klaus-Dietrich Wolff, Thomas Mücke
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6fa66702b88d49c795eef7379c5a49eb
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spelling	oai:doaj.org-article:6fa66702b88d49c795eef7379c5a49eb2021-11-18T08:30:50ZIntroduction of a microsurgical in-vivo embolization-model in rats: the aorta-filter model.1932-620310.1371/journal.pone.0089947https://doaj.org/article/6fa66702b88d49c795eef7379c5a49eb2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24587143/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Vascular thrombosis with subsequent distal embolization remains a critical event for patients. Prevention of this life-threatening event can be achieved pharmacologically or mechanically with intravascular filter systems. The ability to evaluate the risk of embolization of certain techniques and procedures in vascular and microvascular surgery, such as, tissue glue or fibrin based haemostatic agents lacks convincing models. We performed 64 microvascular anastomoses in 44 rats, including 44 micro-pore polyurethane filter-anastomoses and 20 non-filter anastomoses. The rats were re-anesthetized and the aorta was re-exposed and removed four hours, three, seven, fourteen, thirty-one days, and six months postoperatively. The specimens were examined macro- and microscopically with regard to the appearance of the vessel wall, condition of the filter and the amount of thrombembolic material. Typical postoperative histopathological changes in vessel architecture were observed. Media necrosis was the first significant change three days postoperatively. Localized intimal hyperplasia, media necrosis, increase of media fibromyocytes and adventitial hypercellularity were seen to a significant extent at day seven postoperatively. Significant neovascularization of adventitia adjacent to the filter was seen after 14 days. A significant amount of thrombotic material was seen after four hours, three and 14 days interval. Only three intravascular filters became completely occluded (6.82%). The aorta-filter-anastomosis model appeared to be a valid in-vivo model in situations at risk for thrombembolic events, for microsurgical research and allowed sensitive analysis of surgical procedures and protection of the vascularized tissue. It may be suitable for a wide range of in-vivo microvascular experiments particularly in the rat model.Lucas M RitschlAndreas M FichterMonika von DüringDavid A MitchellKlaus-Dietrich WolffThomas MückePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e89947 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucas M Ritschl Andreas M Fichter Monika von Düring David A Mitchell Klaus-Dietrich Wolff Thomas Mücke Introduction of a microsurgical in-vivo embolization-model in rats: the aorta-filter model.
description	Vascular thrombosis with subsequent distal embolization remains a critical event for patients. Prevention of this life-threatening event can be achieved pharmacologically or mechanically with intravascular filter systems. The ability to evaluate the risk of embolization of certain techniques and procedures in vascular and microvascular surgery, such as, tissue glue or fibrin based haemostatic agents lacks convincing models. We performed 64 microvascular anastomoses in 44 rats, including 44 micro-pore polyurethane filter-anastomoses and 20 non-filter anastomoses. The rats were re-anesthetized and the aorta was re-exposed and removed four hours, three, seven, fourteen, thirty-one days, and six months postoperatively. The specimens were examined macro- and microscopically with regard to the appearance of the vessel wall, condition of the filter and the amount of thrombembolic material. Typical postoperative histopathological changes in vessel architecture were observed. Media necrosis was the first significant change three days postoperatively. Localized intimal hyperplasia, media necrosis, increase of media fibromyocytes and adventitial hypercellularity were seen to a significant extent at day seven postoperatively. Significant neovascularization of adventitia adjacent to the filter was seen after 14 days. A significant amount of thrombotic material was seen after four hours, three and 14 days interval. Only three intravascular filters became completely occluded (6.82%). The aorta-filter-anastomosis model appeared to be a valid in-vivo model in situations at risk for thrombembolic events, for microsurgical research and allowed sensitive analysis of surgical procedures and protection of the vascularized tissue. It may be suitable for a wide range of in-vivo microvascular experiments particularly in the rat model.
format	article
author	Lucas M Ritschl Andreas M Fichter Monika von Düring David A Mitchell Klaus-Dietrich Wolff Thomas Mücke
author_facet	Lucas M Ritschl Andreas M Fichter Monika von Düring David A Mitchell Klaus-Dietrich Wolff Thomas Mücke
author_sort	Lucas M Ritschl
title	Introduction of a microsurgical in-vivo embolization-model in rats: the aorta-filter model.
title_short	Introduction of a microsurgical in-vivo embolization-model in rats: the aorta-filter model.
title_full	Introduction of a microsurgical in-vivo embolization-model in rats: the aorta-filter model.
title_fullStr	Introduction of a microsurgical in-vivo embolization-model in rats: the aorta-filter model.
title_full_unstemmed	Introduction of a microsurgical in-vivo embolization-model in rats: the aorta-filter model.
title_sort	introduction of a microsurgical in-vivo embolization-model in rats: the aorta-filter model.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/6fa66702b88d49c795eef7379c5a49eb
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Introduction of a microsurgical in-vivo embolization-model in rats: the aorta-filter model.

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