Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters

Photodynamic therapy (PDT) is a treatment which uses light-activated compounds to produce reactive oxygen species, leading to membrane damage and cell death. Multicellular cancer spheroids are a preferable alternative for PDT evaluation in comparison to monolayer cell cultures due to their ability t...

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Autores principales:	Jose R. Aguilar Cosme, Dan C. Gagui, Helen E. Bryant, Frederik Claeyssens
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	photodynamic therapy cancer spheroids screening morphometric image analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6faf31abb00f4d7f9326770db671e41d
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spelling	oai:doaj.org-article:6faf31abb00f4d7f9326770db671e41d2021-11-18T08:16:56ZMorphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters2296-889X10.3389/fmolb.2021.784962https://doaj.org/article/6faf31abb00f4d7f9326770db671e41d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.784962/fullhttps://doaj.org/toc/2296-889XPhotodynamic therapy (PDT) is a treatment which uses light-activated compounds to produce reactive oxygen species, leading to membrane damage and cell death. Multicellular cancer spheroids are a preferable alternative for PDT evaluation in comparison to monolayer cell cultures due to their ability to better mimic in vivo avascular tumour characteristics such as hypoxia and cell-cell interactions, low cost, and ease of production. However, inconsistent growth kinetics and drug responsiveness causes poor experimental reproducibility and limits their usefulness. Herein, we used image analysis to establish a link between human melanoma C8161 spheroid morphology and drug responsiveness. Spheroids were pre-selected based on sphericity, area, and diameter, reducing variation in experimental groups before treatment. Spheroid morphology after PDT was analyzed using AnaSP and ReViSP, MATLAB-based open-source software, obtaining nine different parameters. Spheroids displayed a linear response between biological assays and morphology, with area (R2 = 0.7219) and volume (R2 = 0.6138) showing the best fit. Sphericity, convexity, and solidity were confirmed as poor standalone indicators of spheroid viability. Our results indicate spheroid morphometric parameters can be used to accurately screen inefficient treatment combinations of novel compounds.Jose R. Aguilar CosmeJose R. Aguilar CosmeDan C. GaguiDan C. GaguiHelen E. BryantFrederik ClaeyssensFrederik ClaeyssensFrontiers Media S.A.articlephotodynamic therapycancer spheroidsscreeningmorphometricimage analysisBiology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	photodynamic therapy cancer spheroids screening morphometric image analysis Biology (General) QH301-705.5
spellingShingle	photodynamic therapy cancer spheroids screening morphometric image analysis Biology (General) QH301-705.5 Jose R. Aguilar Cosme Jose R. Aguilar Cosme Dan C. Gagui Dan C. Gagui Helen E. Bryant Frederik Claeyssens Frederik Claeyssens Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters
description	Photodynamic therapy (PDT) is a treatment which uses light-activated compounds to produce reactive oxygen species, leading to membrane damage and cell death. Multicellular cancer spheroids are a preferable alternative for PDT evaluation in comparison to monolayer cell cultures due to their ability to better mimic in vivo avascular tumour characteristics such as hypoxia and cell-cell interactions, low cost, and ease of production. However, inconsistent growth kinetics and drug responsiveness causes poor experimental reproducibility and limits their usefulness. Herein, we used image analysis to establish a link between human melanoma C8161 spheroid morphology and drug responsiveness. Spheroids were pre-selected based on sphericity, area, and diameter, reducing variation in experimental groups before treatment. Spheroid morphology after PDT was analyzed using AnaSP and ReViSP, MATLAB-based open-source software, obtaining nine different parameters. Spheroids displayed a linear response between biological assays and morphology, with area (R2 = 0.7219) and volume (R2 = 0.6138) showing the best fit. Sphericity, convexity, and solidity were confirmed as poor standalone indicators of spheroid viability. Our results indicate spheroid morphometric parameters can be used to accurately screen inefficient treatment combinations of novel compounds.
format	article
author	Jose R. Aguilar Cosme Jose R. Aguilar Cosme Dan C. Gagui Dan C. Gagui Helen E. Bryant Frederik Claeyssens Frederik Claeyssens
author_facet	Jose R. Aguilar Cosme Jose R. Aguilar Cosme Dan C. Gagui Dan C. Gagui Helen E. Bryant Frederik Claeyssens Frederik Claeyssens
author_sort	Jose R. Aguilar Cosme
title	Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters
title_short	Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters
title_full	Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters
title_fullStr	Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters
title_full_unstemmed	Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters
title_sort	morphological response in cancer spheroids for screening photodynamic therapy parameters
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6faf31abb00f4d7f9326770db671e41d
work_keys_str_mv	AT joseraguilarcosme morphologicalresponseincancerspheroidsforscreeningphotodynamictherapyparameters AT joseraguilarcosme morphologicalresponseincancerspheroidsforscreeningphotodynamictherapyparameters AT dancgagui morphologicalresponseincancerspheroidsforscreeningphotodynamictherapyparameters AT dancgagui morphologicalresponseincancerspheroidsforscreeningphotodynamictherapyparameters AT helenebryant morphologicalresponseincancerspheroidsforscreeningphotodynamictherapyparameters AT frederikclaeyssens morphologicalresponseincancerspheroidsforscreeningphotodynamictherapyparameters AT frederikclaeyssens morphologicalresponseincancerspheroidsforscreeningphotodynamictherapyparameters
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Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters

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