Regulation of ytfK by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation YtfK Responds to Glucose Exhaustion

Guanosine penta- or tetraphosphate (known as (p)ppGpp) serves as second messenger to respond to nutrient downshift and other environmental stresses, a phenomenon called stringent response. Accumulation of (p)ppGpp promotes the coordinated inhibition of macromolecule synthesis, as well as the activat...

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Autores principales:	Laura Meyer, Elsa Germain, Etienne Maisonneuve
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	(p)ppGpp stringent response ytfK cAMP CRP glucose starvation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6fb0ee5ef7e74e9c9429cbd52d4c93f7
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spelling	oai:doaj.org-article:6fb0ee5ef7e74e9c9429cbd52d4c93f72021-11-04T10:14:57ZRegulation of ytfK by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation YtfK Responds to Glucose Exhaustion1664-302X10.3389/fmicb.2021.775164https://doaj.org/article/6fb0ee5ef7e74e9c9429cbd52d4c93f72021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.775164/fullhttps://doaj.org/toc/1664-302XGuanosine penta- or tetraphosphate (known as (p)ppGpp) serves as second messenger to respond to nutrient downshift and other environmental stresses, a phenomenon called stringent response. Accumulation of (p)ppGpp promotes the coordinated inhibition of macromolecule synthesis, as well as the activation of stress response pathways to cope and adapt to harmful conditions. In Escherichia coli, the (p)ppGpp level is tightly regulated by two enzymes, the (p)ppGpp synthetase RelA and the bifunctional synthetase/hydrolase SpoT. We recently identified the small protein YtfK as a key regulator of SpoT-mediated activation of stringent response in E. coli. Here, we further characterized the regulation of ytfK. We observed that ytfK is subjected to catabolite repression and is positively regulated by the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex. Importantly, YtfK contributes to SpoT-dependent accumulation of (p)ppGpp and cell survival in response to glucose starvation. Therefore, regulation of ytfK by the cAMP-CRP appears important to adjust (p)ppGpp level and coordinate cellular metabolism in response to glucose availability.Laura MeyerElsa GermainEtienne MaisonneuveFrontiers Media S.A.article(p)ppGppstringent responseytfKcAMPCRPglucose starvationMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	(p)ppGpp stringent response ytfK cAMP CRP glucose starvation Microbiology QR1-502
spellingShingle	(p)ppGpp stringent response ytfK cAMP CRP glucose starvation Microbiology QR1-502 Laura Meyer Elsa Germain Etienne Maisonneuve Regulation of ytfK by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation YtfK Responds to Glucose Exhaustion
description	Guanosine penta- or tetraphosphate (known as (p)ppGpp) serves as second messenger to respond to nutrient downshift and other environmental stresses, a phenomenon called stringent response. Accumulation of (p)ppGpp promotes the coordinated inhibition of macromolecule synthesis, as well as the activation of stress response pathways to cope and adapt to harmful conditions. In Escherichia coli, the (p)ppGpp level is tightly regulated by two enzymes, the (p)ppGpp synthetase RelA and the bifunctional synthetase/hydrolase SpoT. We recently identified the small protein YtfK as a key regulator of SpoT-mediated activation of stringent response in E. coli. Here, we further characterized the regulation of ytfK. We observed that ytfK is subjected to catabolite repression and is positively regulated by the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex. Importantly, YtfK contributes to SpoT-dependent accumulation of (p)ppGpp and cell survival in response to glucose starvation. Therefore, regulation of ytfK by the cAMP-CRP appears important to adjust (p)ppGpp level and coordinate cellular metabolism in response to glucose availability.
format	article
author	Laura Meyer Elsa Germain Etienne Maisonneuve
author_facet	Laura Meyer Elsa Germain Etienne Maisonneuve
author_sort	Laura Meyer
title	Regulation of ytfK by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation YtfK Responds to Glucose Exhaustion
title_short	Regulation of ytfK by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation YtfK Responds to Glucose Exhaustion
title_full	Regulation of ytfK by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation YtfK Responds to Glucose Exhaustion
title_fullStr	Regulation of ytfK by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation YtfK Responds to Glucose Exhaustion
title_full_unstemmed	Regulation of ytfK by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation YtfK Responds to Glucose Exhaustion
title_sort	regulation of ytfk by camp-crp contributes to spot-dependent accumulation of (p)ppgpp in response to carbon starvation ytfk responds to glucose exhaustion
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6fb0ee5ef7e74e9c9429cbd52d4c93f7
work_keys_str_mv	AT laurameyer regulationofytfkbycampcrpcontributestospotdependentaccumulationofpppgppinresponsetocarbonstarvationytfkrespondstoglucoseexhaustion AT elsagermain regulationofytfkbycampcrpcontributestospotdependentaccumulationofpppgppinresponsetocarbonstarvationytfkrespondstoglucoseexhaustion AT etiennemaisonneuve regulationofytfkbycampcrpcontributestospotdependentaccumulationofpppgppinresponsetocarbonstarvationytfkrespondstoglucoseexhaustion
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Regulation of ytfK by cAMP-CRP Contributes to SpoT-Dependent Accumulation of (p)ppGpp in Response to Carbon Starvation YtfK Responds to Glucose Exhaustion

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