Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells

Abstract So far, the development of a unique strategy for specific biomolecules activity monitoring and precise drugs release in cancerous cells is still challenging. Here, we designed a conformation-switchable smart nanoprobe to monitor telomerase activity and to enable activity-triggered drug rele...

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Autores principales:	Hai Shi, Tao Gao, Liu Shi, Tianshu Chen, Yang Xiang, Yuanyang Li, Genxi Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Nanoprobes Telomerase Activity TRAP Reaction Buffer Telomerase Extract Telomeric Repeat Amplification Protocol (TRAP) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6fb2933b73d94e3da40323bd09cfe140
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spelling	oai:doaj.org-article:6fb2933b73d94e3da40323bd09cfe1402021-12-02T15:08:41ZMolecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells10.1038/s41598-018-34670-72045-2322https://doaj.org/article/6fb2933b73d94e3da40323bd09cfe1402018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34670-7https://doaj.org/toc/2045-2322Abstract So far, the development of a unique strategy for specific biomolecules activity monitoring and precise drugs release in cancerous cells is still challenging. Here, we designed a conformation-switchable smart nanoprobe to monitor telomerase activity and to enable activity-triggered drug release in cancerous cells. The straightforward nanoprobe contained a gold nanoparticle (AuNP) core and a dense layer of 5-carboxyfluorescein (FAM)-labeled hairpin DNA shell. The 3′ region of hairpin DNA sequence could function as the telomerase primer to be elongated in the presence of telomerase, resulting in the conformational switch of hairpin DNA. As a result, the FAM fluorescence was activated and the anticancer drug doxorubicin (Dox) molecules which intercalated into the stem region of the hairpin DNA sequence were released into cancerous cells simultaneously. The smart method could specifically distinguish cancerous cells from normal cells based on telomerase activity. It also showed a good performance for monitoring telomerase activity in the cytoplasm by molecular imaging and precise release of Dox triggered by telomerase activity in cancerous cells. These advantages may offer a great potential of this method for monitoring telomerase activity in cancer progression and estimating therapeutic effect.Hai ShiTao GaoLiu ShiTianshu ChenYang XiangYuanyang LiGenxi LiNature PortfolioarticleNanoprobesTelomerase ActivityTRAP Reaction BufferTelomerase ExtractTelomeric Repeat Amplification Protocol (TRAP)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanoprobes Telomerase Activity TRAP Reaction Buffer Telomerase Extract Telomeric Repeat Amplification Protocol (TRAP) Medicine R Science Q
spellingShingle	Nanoprobes Telomerase Activity TRAP Reaction Buffer Telomerase Extract Telomeric Repeat Amplification Protocol (TRAP) Medicine R Science Q Hai Shi Tao Gao Liu Shi Tianshu Chen Yang Xiang Yuanyang Li Genxi Li Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells
description	Abstract So far, the development of a unique strategy for specific biomolecules activity monitoring and precise drugs release in cancerous cells is still challenging. Here, we designed a conformation-switchable smart nanoprobe to monitor telomerase activity and to enable activity-triggered drug release in cancerous cells. The straightforward nanoprobe contained a gold nanoparticle (AuNP) core and a dense layer of 5-carboxyfluorescein (FAM)-labeled hairpin DNA shell. The 3′ region of hairpin DNA sequence could function as the telomerase primer to be elongated in the presence of telomerase, resulting in the conformational switch of hairpin DNA. As a result, the FAM fluorescence was activated and the anticancer drug doxorubicin (Dox) molecules which intercalated into the stem region of the hairpin DNA sequence were released into cancerous cells simultaneously. The smart method could specifically distinguish cancerous cells from normal cells based on telomerase activity. It also showed a good performance for monitoring telomerase activity in the cytoplasm by molecular imaging and precise release of Dox triggered by telomerase activity in cancerous cells. These advantages may offer a great potential of this method for monitoring telomerase activity in cancer progression and estimating therapeutic effect.
format	article
author	Hai Shi Tao Gao Liu Shi Tianshu Chen Yang Xiang Yuanyang Li Genxi Li
author_facet	Hai Shi Tao Gao Liu Shi Tianshu Chen Yang Xiang Yuanyang Li Genxi Li
author_sort	Hai Shi
title	Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells
title_short	Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells
title_full	Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells
title_fullStr	Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells
title_full_unstemmed	Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells
title_sort	molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6fb2933b73d94e3da40323bd09cfe140
work_keys_str_mv	AT haishi molecularimagingoftelomeraseandtheenzymeactivitytriggereddrugreleasebyusingaconformationswitchablenanoprobeincancerouscells AT taogao molecularimagingoftelomeraseandtheenzymeactivitytriggereddrugreleasebyusingaconformationswitchablenanoprobeincancerouscells AT liushi molecularimagingoftelomeraseandtheenzymeactivitytriggereddrugreleasebyusingaconformationswitchablenanoprobeincancerouscells AT tianshuchen molecularimagingoftelomeraseandtheenzymeactivitytriggereddrugreleasebyusingaconformationswitchablenanoprobeincancerouscells AT yangxiang molecularimagingoftelomeraseandtheenzymeactivitytriggereddrugreleasebyusingaconformationswitchablenanoprobeincancerouscells AT yuanyangli molecularimagingoftelomeraseandtheenzymeactivitytriggereddrugreleasebyusingaconformationswitchablenanoprobeincancerouscells AT genxili molecularimagingoftelomeraseandtheenzymeactivitytriggereddrugreleasebyusingaconformationswitchablenanoprobeincancerouscells
_version_	1718388064761413632

Molecular imaging of telomerase and the enzyme activity-triggered drug release by using a conformation-switchable nanoprobe in cancerous cells

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