Multiplexed labeling of cellular proteins with split fluorescent protein tags

Tamura et al. expand the split fluorescent protein system to multi-colour imaging (six-colours). Their multiplexing approach, using the orthogonal split FP systems, allows simultaneous imaging of four distinct proteins in single cells.

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Detalles Bibliográficos
Autores principales: Ryo Tamura, Fangchao Jiang, Jin Xie, Daichi Kamiyama
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/6fb481e204bd4378ae2679528292dc50
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Sumario:Tamura et al. expand the split fluorescent protein system to multi-colour imaging (six-colours). Their multiplexing approach, using the orthogonal split FP systems, allows simultaneous imaging of four distinct proteins in single cells.