Multiplexed labeling of cellular proteins with split fluorescent protein tags
Tamura et al. expand the split fluorescent protein system to multi-colour imaging (six-colours). Their multiplexing approach, using the orthogonal split FP systems, allows simultaneous imaging of four distinct proteins in single cells.
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Nature Portfolio
2021
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oai:doaj.org-article:6fb481e204bd4378ae2679528292dc502021-12-02T13:33:50ZMultiplexed labeling of cellular proteins with split fluorescent protein tags10.1038/s42003-021-01780-42399-3642https://doaj.org/article/6fb481e204bd4378ae2679528292dc502021-02-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01780-4https://doaj.org/toc/2399-3642Tamura et al. expand the split fluorescent protein system to multi-colour imaging (six-colours). Their multiplexing approach, using the orthogonal split FP systems, allows simultaneous imaging of four distinct proteins in single cells.Ryo TamuraFangchao JiangJin XieDaichi KamiyamaNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-8 (2021) |
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DOAJ |
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DOAJ |
language |
EN |
topic |
Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 Ryo Tamura Fangchao Jiang Jin Xie Daichi Kamiyama Multiplexed labeling of cellular proteins with split fluorescent protein tags |
description |
Tamura et al. expand the split fluorescent protein system to multi-colour imaging (six-colours). Their multiplexing approach, using the orthogonal split FP systems, allows simultaneous imaging of four distinct proteins in single cells. |
format |
article |
author |
Ryo Tamura Fangchao Jiang Jin Xie Daichi Kamiyama |
author_facet |
Ryo Tamura Fangchao Jiang Jin Xie Daichi Kamiyama |
author_sort |
Ryo Tamura |
title |
Multiplexed labeling of cellular proteins with split fluorescent protein tags |
title_short |
Multiplexed labeling of cellular proteins with split fluorescent protein tags |
title_full |
Multiplexed labeling of cellular proteins with split fluorescent protein tags |
title_fullStr |
Multiplexed labeling of cellular proteins with split fluorescent protein tags |
title_full_unstemmed |
Multiplexed labeling of cellular proteins with split fluorescent protein tags |
title_sort |
multiplexed labeling of cellular proteins with split fluorescent protein tags |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/6fb481e204bd4378ae2679528292dc50 |
work_keys_str_mv |
AT ryotamura multiplexedlabelingofcellularproteinswithsplitfluorescentproteintags AT fangchaojiang multiplexedlabelingofcellularproteinswithsplitfluorescentproteintags AT jinxie multiplexedlabelingofcellularproteinswithsplitfluorescentproteintags AT daichikamiyama multiplexedlabelingofcellularproteinswithsplitfluorescentproteintags |
_version_ |
1718392860162654208 |