Multiplexed labeling of cellular proteins with split fluorescent protein tags

Tamura et al. expand the split fluorescent protein system to multi-colour imaging (six-colours). Their multiplexing approach, using the orthogonal split FP systems, allows simultaneous imaging of four distinct proteins in single cells.

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Autores principales: Ryo Tamura, Fangchao Jiang, Jin Xie, Daichi Kamiyama
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/6fb481e204bd4378ae2679528292dc50
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spelling oai:doaj.org-article:6fb481e204bd4378ae2679528292dc502021-12-02T13:33:50ZMultiplexed labeling of cellular proteins with split fluorescent protein tags10.1038/s42003-021-01780-42399-3642https://doaj.org/article/6fb481e204bd4378ae2679528292dc502021-02-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01780-4https://doaj.org/toc/2399-3642Tamura et al. expand the split fluorescent protein system to multi-colour imaging (six-colours). Their multiplexing approach, using the orthogonal split FP systems, allows simultaneous imaging of four distinct proteins in single cells.Ryo TamuraFangchao JiangJin XieDaichi KamiyamaNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-8 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Ryo Tamura
Fangchao Jiang
Jin Xie
Daichi Kamiyama
Multiplexed labeling of cellular proteins with split fluorescent protein tags
description Tamura et al. expand the split fluorescent protein system to multi-colour imaging (six-colours). Their multiplexing approach, using the orthogonal split FP systems, allows simultaneous imaging of four distinct proteins in single cells.
format article
author Ryo Tamura
Fangchao Jiang
Jin Xie
Daichi Kamiyama
author_facet Ryo Tamura
Fangchao Jiang
Jin Xie
Daichi Kamiyama
author_sort Ryo Tamura
title Multiplexed labeling of cellular proteins with split fluorescent protein tags
title_short Multiplexed labeling of cellular proteins with split fluorescent protein tags
title_full Multiplexed labeling of cellular proteins with split fluorescent protein tags
title_fullStr Multiplexed labeling of cellular proteins with split fluorescent protein tags
title_full_unstemmed Multiplexed labeling of cellular proteins with split fluorescent protein tags
title_sort multiplexed labeling of cellular proteins with split fluorescent protein tags
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/6fb481e204bd4378ae2679528292dc50
work_keys_str_mv AT ryotamura multiplexedlabelingofcellularproteinswithsplitfluorescentproteintags
AT fangchaojiang multiplexedlabelingofcellularproteinswithsplitfluorescentproteintags
AT jinxie multiplexedlabelingofcellularproteinswithsplitfluorescentproteintags
AT daichikamiyama multiplexedlabelingofcellularproteinswithsplitfluorescentproteintags
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