CircHIPK3 Alleviates High Glucose Toxicity to Human Renal Tubular Epithelial HK-2 Cells Through Regulation of miR-326/miR-487a-3p/SIRT1

Langen Zhuang, Ziwei Wang, Xiaolei Hu, Qingqing Yang, Xiaoyan Pei, Guoxi Jin Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004 Anhui, People’s Republic of ChinaCorrespondence: Guoxi JinDepartment of Endocrinology, The First Affiliated Hosp...

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Autores principales:	Zhuang L, Wang Z, Hu X, Yang Q, Pei X, Jin G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	diabetic nephropathy high glucose circhipk3 mir-326 mir-487a-3p sirt1 Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/6fb89ca4390646469b77ffa5738f27da
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spelling	oai:doaj.org-article:6fb89ca4390646469b77ffa5738f27da2021-12-02T14:16:01ZCircHIPK3 Alleviates High Glucose Toxicity to Human Renal Tubular Epithelial HK-2 Cells Through Regulation of miR-326/miR-487a-3p/SIRT11178-7007https://doaj.org/article/6fb89ca4390646469b77ffa5738f27da2021-02-01T00:00:00Zhttps://www.dovepress.com/circhipk3-alleviates-high-glucose-toxicity-to-human-renal-tubular-epit-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Langen Zhuang, Ziwei Wang, Xiaolei Hu, Qingqing Yang, Xiaoyan Pei, Guoxi Jin Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004 Anhui, People’s Republic of ChinaCorrespondence: Guoxi JinDepartment of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, 233004, People’s Republic of ChinaTel +86-18096530238Email guoxijinnfm@163.comBackground: The intervention of circular RNA HIPK3 (circHIPK3) in diabetes has drawn increasing attention in recent years. However, the underlying mechanism of circHIPK3 in diabetic nephropathy (DN) has not been fully elucidated. Thus, the current study aims to investigate the role of circHIPK3 in high glucose (HG)-induced toxicity to human renal tubular epithelial HK-2 cells.Methods: The expression of circHIPK3 in HK-2 cells induced by HG was determined by qRT-PCR and Western blot. The regulatory effects of circHIPK3 and miR-326/miR-487a-3p on cells proliferative and apoptosis were evaluated by CCK-8 and flow cytometry. Dual-luciferase reporter assay was applied to predict the target genes of miR-326 or miR-487a-3p.Results: Expression level of circHIPK3 in HK-2 cells was remarkably decreased after the treatment of HG. The overexpression of circHIPK3 effectively reversed the HG-induced HK-2 cell proliferation inhibition and apoptosis. Furthermore, SIRT1 was confirmed to be the target gene of miR-326 and miR-487a-3p, which were showed to be the downstream genes of circHIPK3. The silencing of miR-326 or miR-487a-3p was also proved to induce proliferation and reduce apoptosis in HG-induced HK-2 cells.Conclusion: Our data suggest that overexpression of circHIPK3 can attenuate the proliferation inhibition of HK-2 induced by HG and inhibit apoptosis through sponging miR-326 or miR-487a-3p to regulate SIRT1.Keywords: diabetic nephropathy, high glucose, circHIPK3, miR-326, miR-487a-3p, SIRT1Zhuang LWang ZHu XYang QPei XJin GDove Medical Pressarticlediabetic nephropathyhigh glucosecirchipk3mir-326mir-487a-3psirt1Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 729-740 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetic nephropathy high glucose circhipk3 mir-326 mir-487a-3p sirt1 Specialties of internal medicine RC581-951
spellingShingle	diabetic nephropathy high glucose circhipk3 mir-326 mir-487a-3p sirt1 Specialties of internal medicine RC581-951 Zhuang L Wang Z Hu X Yang Q Pei X Jin G CircHIPK3 Alleviates High Glucose Toxicity to Human Renal Tubular Epithelial HK-2 Cells Through Regulation of miR-326/miR-487a-3p/SIRT1
description	Langen Zhuang, Ziwei Wang, Xiaolei Hu, Qingqing Yang, Xiaoyan Pei, Guoxi Jin Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004 Anhui, People’s Republic of ChinaCorrespondence: Guoxi JinDepartment of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, 233004, People’s Republic of ChinaTel +86-18096530238Email guoxijinnfm@163.comBackground: The intervention of circular RNA HIPK3 (circHIPK3) in diabetes has drawn increasing attention in recent years. However, the underlying mechanism of circHIPK3 in diabetic nephropathy (DN) has not been fully elucidated. Thus, the current study aims to investigate the role of circHIPK3 in high glucose (HG)-induced toxicity to human renal tubular epithelial HK-2 cells.Methods: The expression of circHIPK3 in HK-2 cells induced by HG was determined by qRT-PCR and Western blot. The regulatory effects of circHIPK3 and miR-326/miR-487a-3p on cells proliferative and apoptosis were evaluated by CCK-8 and flow cytometry. Dual-luciferase reporter assay was applied to predict the target genes of miR-326 or miR-487a-3p.Results: Expression level of circHIPK3 in HK-2 cells was remarkably decreased after the treatment of HG. The overexpression of circHIPK3 effectively reversed the HG-induced HK-2 cell proliferation inhibition and apoptosis. Furthermore, SIRT1 was confirmed to be the target gene of miR-326 and miR-487a-3p, which were showed to be the downstream genes of circHIPK3. The silencing of miR-326 or miR-487a-3p was also proved to induce proliferation and reduce apoptosis in HG-induced HK-2 cells.Conclusion: Our data suggest that overexpression of circHIPK3 can attenuate the proliferation inhibition of HK-2 induced by HG and inhibit apoptosis through sponging miR-326 or miR-487a-3p to regulate SIRT1.Keywords: diabetic nephropathy, high glucose, circHIPK3, miR-326, miR-487a-3p, SIRT1
format	article
author	Zhuang L Wang Z Hu X Yang Q Pei X Jin G
author_facet	Zhuang L Wang Z Hu X Yang Q Pei X Jin G
author_sort	Zhuang L
title	CircHIPK3 Alleviates High Glucose Toxicity to Human Renal Tubular Epithelial HK-2 Cells Through Regulation of miR-326/miR-487a-3p/SIRT1
title_short	CircHIPK3 Alleviates High Glucose Toxicity to Human Renal Tubular Epithelial HK-2 Cells Through Regulation of miR-326/miR-487a-3p/SIRT1
title_full	CircHIPK3 Alleviates High Glucose Toxicity to Human Renal Tubular Epithelial HK-2 Cells Through Regulation of miR-326/miR-487a-3p/SIRT1
title_fullStr	CircHIPK3 Alleviates High Glucose Toxicity to Human Renal Tubular Epithelial HK-2 Cells Through Regulation of miR-326/miR-487a-3p/SIRT1
title_full_unstemmed	CircHIPK3 Alleviates High Glucose Toxicity to Human Renal Tubular Epithelial HK-2 Cells Through Regulation of miR-326/miR-487a-3p/SIRT1
title_sort	circhipk3 alleviates high glucose toxicity to human renal tubular epithelial hk-2 cells through regulation of mir-326/mir-487a-3p/sirt1
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/6fb89ca4390646469b77ffa5738f27da
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CircHIPK3 Alleviates High Glucose Toxicity to Human Renal Tubular Epithelial HK-2 Cells Through Regulation of miR-326/miR-487a-3p/SIRT1

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