Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments

Ribosome profiling has become the gold standard to analyze mRNA translation dynamics, and the translation inhibitor cycloheximide (CHX) is often used in its application. Here the authors systematically demonstrate that CHX does not bias the outcome of ribosome profiling experiments in most organisms...

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Autores principales:	Puneet Sharma, Jie Wu, Benedikt S. Nilges, Sebastian A. Leidel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6fc16742fd5346298a852f3763b9b39b
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spelling	oai:doaj.org-article:6fc16742fd5346298a852f3763b9b39b2021-12-02T18:53:20ZHumans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments10.1038/s41467-021-25411-y2041-1723https://doaj.org/article/6fc16742fd5346298a852f3763b9b39b2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25411-yhttps://doaj.org/toc/2041-1723Ribosome profiling has become the gold standard to analyze mRNA translation dynamics, and the translation inhibitor cycloheximide (CHX) is often used in its application. Here the authors systematically demonstrate that CHX does not bias the outcome of ribosome profiling experiments in most organisms.Puneet SharmaJie WuBenedikt S. NilgesSebastian A. LeidelNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Puneet Sharma Jie Wu Benedikt S. Nilges Sebastian A. Leidel Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments
description	Ribosome profiling has become the gold standard to analyze mRNA translation dynamics, and the translation inhibitor cycloheximide (CHX) is often used in its application. Here the authors systematically demonstrate that CHX does not bias the outcome of ribosome profiling experiments in most organisms.
format	article
author	Puneet Sharma Jie Wu Benedikt S. Nilges Sebastian A. Leidel
author_facet	Puneet Sharma Jie Wu Benedikt S. Nilges Sebastian A. Leidel
author_sort	Puneet Sharma
title	Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments
title_short	Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments
title_full	Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments
title_fullStr	Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments
title_full_unstemmed	Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments
title_sort	humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6fc16742fd5346298a852f3763b9b39b
work_keys_str_mv	AT puneetsharma humansandothercommonlyusedmodelorganismsareresistanttocycloheximidemediatedbiasesinribosomeprofilingexperiments AT jiewu humansandothercommonlyusedmodelorganismsareresistanttocycloheximidemediatedbiasesinribosomeprofilingexperiments AT benediktsnilges humansandothercommonlyusedmodelorganismsareresistanttocycloheximidemediatedbiasesinribosomeprofilingexperiments AT sebastianaleidel humansandothercommonlyusedmodelorganismsareresistanttocycloheximidemediatedbiasesinribosomeprofilingexperiments
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Humans and other commonly used model organisms are resistant to cycloheximide-mediated biases in ribosome profiling experiments

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