Chromatin organization regulates viral egress dynamics

Abstract Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion...

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Autores principales:	Vesa Aho, Markko Myllys, Visa Ruokolainen, Satu Hakanen, Elina Mäntylä, Jori Virtanen, Veijo Hukkanen, Thomas Kühn, Jussi Timonen, Keijo Mattila, Carolyn A. Larabell, Maija Vihinen-Ranta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6fc9b143eefe40759c570e5b44160ee8
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spelling	oai:doaj.org-article:6fc9b143eefe40759c570e5b44160ee82021-12-02T16:06:55ZChromatin organization regulates viral egress dynamics10.1038/s41598-017-03630-y2045-2322https://doaj.org/article/6fc9b143eefe40759c570e5b44160ee82017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03630-yhttps://doaj.org/toc/2045-2322Abstract Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1–sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.Vesa AhoMarkko MyllysVisa RuokolainenSatu HakanenElina MäntyläJori VirtanenVeijo HukkanenThomas KühnJussi TimonenKeijo MattilaCarolyn A. LarabellMaija Vihinen-RantaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vesa Aho Markko Myllys Visa Ruokolainen Satu Hakanen Elina Mäntylä Jori Virtanen Veijo Hukkanen Thomas Kühn Jussi Timonen Keijo Mattila Carolyn A. Larabell Maija Vihinen-Ranta Chromatin organization regulates viral egress dynamics
description	Abstract Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1–sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.
format	article
author	Vesa Aho Markko Myllys Visa Ruokolainen Satu Hakanen Elina Mäntylä Jori Virtanen Veijo Hukkanen Thomas Kühn Jussi Timonen Keijo Mattila Carolyn A. Larabell Maija Vihinen-Ranta
author_facet	Vesa Aho Markko Myllys Visa Ruokolainen Satu Hakanen Elina Mäntylä Jori Virtanen Veijo Hukkanen Thomas Kühn Jussi Timonen Keijo Mattila Carolyn A. Larabell Maija Vihinen-Ranta
author_sort	Vesa Aho
title	Chromatin organization regulates viral egress dynamics
title_short	Chromatin organization regulates viral egress dynamics
title_full	Chromatin organization regulates viral egress dynamics
title_fullStr	Chromatin organization regulates viral egress dynamics
title_full_unstemmed	Chromatin organization regulates viral egress dynamics
title_sort	chromatin organization regulates viral egress dynamics
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6fc9b143eefe40759c570e5b44160ee8
work_keys_str_mv	AT vesaaho chromatinorganizationregulatesviralegressdynamics AT markkomyllys chromatinorganizationregulatesviralegressdynamics AT visaruokolainen chromatinorganizationregulatesviralegressdynamics AT satuhakanen chromatinorganizationregulatesviralegressdynamics AT elinamantyla chromatinorganizationregulatesviralegressdynamics AT jorivirtanen chromatinorganizationregulatesviralegressdynamics AT veijohukkanen chromatinorganizationregulatesviralegressdynamics AT thomaskuhn chromatinorganizationregulatesviralegressdynamics AT jussitimonen chromatinorganizationregulatesviralegressdynamics AT keijomattila chromatinorganizationregulatesviralegressdynamics AT carolynalarabell chromatinorganizationregulatesviralegressdynamics AT maijavihinenranta chromatinorganizationregulatesviralegressdynamics
_version_	1718384781646888960

Chromatin organization regulates viral egress dynamics

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