Mycobacterium tuberculosis requires the ECF sigma factor SigE to arrest phagosome maturation.

SigE represents one of the best characterized alternative sigma factors of Mycobacterium tuberculosis, playing a major role in the response to several environmental stresses and essential for growth in macrophages and virulence. In previous work we demonstrated that a mutant of M. tuberculosis in wh...

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Autores principales:	Stefano Casonato, Roberta Provvedi, Elisa Dainese, Giorgio Palù, Riccardo Manganelli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6fcd50e8d2da4703bd679c34f6872443
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spelling	oai:doaj.org-article:6fcd50e8d2da4703bd679c34f68724432021-11-25T05:58:33ZMycobacterium tuberculosis requires the ECF sigma factor SigE to arrest phagosome maturation.1932-620310.1371/journal.pone.0108893https://doaj.org/article/6fcd50e8d2da4703bd679c34f68724432014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108893https://doaj.org/toc/1932-6203SigE represents one of the best characterized alternative sigma factors of Mycobacterium tuberculosis, playing a major role in the response to several environmental stresses and essential for growth in macrophages and virulence. In previous work we demonstrated that a mutant of M. tuberculosis in which the sigE gene was disrupted by a cassette conferring hygromycin resistance is a promising vaccine candidate conferring better protection than Mycobacterium bovis BCG in a mouse model of infection. In this work we describe the construction of a new unmarked mutant in which the entire sigE gene was disrupted in order to fulfill the requirements of the Geneva consensus to enter clinical trials. After showing that the phenotype of this mutant is superimposable to that of the previous one, we further characterized the role of SigE in the M tuberculosis intracellular behavior showing that it is dispensable for replication in human pneumocytes, while it is essential for the arrest of phagosome maturation in THP-1-derived macrophages.Stefano CasonatoRoberta ProvvediElisa DaineseGiorgio PalùRiccardo ManganelliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108893 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefano Casonato Roberta Provvedi Elisa Dainese Giorgio Palù Riccardo Manganelli Mycobacterium tuberculosis requires the ECF sigma factor SigE to arrest phagosome maturation.
description	SigE represents one of the best characterized alternative sigma factors of Mycobacterium tuberculosis, playing a major role in the response to several environmental stresses and essential for growth in macrophages and virulence. In previous work we demonstrated that a mutant of M. tuberculosis in which the sigE gene was disrupted by a cassette conferring hygromycin resistance is a promising vaccine candidate conferring better protection than Mycobacterium bovis BCG in a mouse model of infection. In this work we describe the construction of a new unmarked mutant in which the entire sigE gene was disrupted in order to fulfill the requirements of the Geneva consensus to enter clinical trials. After showing that the phenotype of this mutant is superimposable to that of the previous one, we further characterized the role of SigE in the M tuberculosis intracellular behavior showing that it is dispensable for replication in human pneumocytes, while it is essential for the arrest of phagosome maturation in THP-1-derived macrophages.
format	article
author	Stefano Casonato Roberta Provvedi Elisa Dainese Giorgio Palù Riccardo Manganelli
author_facet	Stefano Casonato Roberta Provvedi Elisa Dainese Giorgio Palù Riccardo Manganelli
author_sort	Stefano Casonato
title	Mycobacterium tuberculosis requires the ECF sigma factor SigE to arrest phagosome maturation.
title_short	Mycobacterium tuberculosis requires the ECF sigma factor SigE to arrest phagosome maturation.
title_full	Mycobacterium tuberculosis requires the ECF sigma factor SigE to arrest phagosome maturation.
title_fullStr	Mycobacterium tuberculosis requires the ECF sigma factor SigE to arrest phagosome maturation.
title_full_unstemmed	Mycobacterium tuberculosis requires the ECF sigma factor SigE to arrest phagosome maturation.
title_sort	mycobacterium tuberculosis requires the ecf sigma factor sige to arrest phagosome maturation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/6fcd50e8d2da4703bd679c34f6872443
work_keys_str_mv	AT stefanocasonato mycobacteriumtuberculosisrequirestheecfsigmafactorsigetoarrestphagosomematuration AT robertaprovvedi mycobacteriumtuberculosisrequirestheecfsigmafactorsigetoarrestphagosomematuration AT elisadainese mycobacteriumtuberculosisrequirestheecfsigmafactorsigetoarrestphagosomematuration AT giorgiopalu mycobacteriumtuberculosisrequirestheecfsigmafactorsigetoarrestphagosomematuration AT riccardomanganelli mycobacteriumtuberculosisrequirestheecfsigmafactorsigetoarrestphagosomematuration
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Mycobacterium tuberculosis requires the ECF sigma factor SigE to arrest phagosome maturation.

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