Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.

Post-weaning diarrhea (PWD) in piglets is a major problem in piggeries worldwide and results in severe economic losses. Infection with Enterotoxigenic Escherichia coli (ETEC) is the key culprit for the PWD disease. F4 fimbriae of ETEC are highly stable proteinaceous polymers, mainly composed of the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Igor Kolotilin, Angelo Kaldis, Bert Devriendt, Jussi Joensuu, Eric Cox, Rima Menassa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6fdefbeda3d84c26990daac3e2cbbbcb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6fdefbeda3d84c26990daac3e2cbbbcb
record_format	dspace
spelling	oai:doaj.org-article:6fdefbeda3d84c26990daac3e2cbbbcb2021-11-18T07:09:46ZProduction of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.1932-620310.1371/journal.pone.0042405https://doaj.org/article/6fdefbeda3d84c26990daac3e2cbbbcb2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22879967/?tool=EBIhttps://doaj.org/toc/1932-6203Post-weaning diarrhea (PWD) in piglets is a major problem in piggeries worldwide and results in severe economic losses. Infection with Enterotoxigenic Escherichia coli (ETEC) is the key culprit for the PWD disease. F4 fimbriae of ETEC are highly stable proteinaceous polymers, mainly composed of the major structural subunit FaeG, with a capacity to evoke mucosal immune responses, thus demonstrating a potential to act as an oral vaccine against ETEC-induced porcine PWD. In this study we used a transplastomic approach in tobacco to produce a recombinant variant of the FaeG protein, rFaeG(ntd/dsc), engineered for expression as a stable monomer by N-terminal deletion and donor strand-complementation (ntd/dsc). The generated transplastomic tobacco plants accumulated up to 2.0 g rFaeG(ntd/dsc) per 1 kg fresh leaf tissue (more than 1% of dry leaf tissue) and showed normal phenotype indistinguishable from wild type untransformed plants. We determined that chloroplast-produced rFaeG(ntd/dsc) protein retained the key properties of an oral vaccine, i.e. binding to porcine intestinal F4 receptors (F4R), and inhibition of the F4-possessing (F4+) ETEC attachment to F4R. Additionally, the plant biomass matrix was shown to delay degradation of the chloroplast-produced rFaeG(ntd/dsc) in gastrointestinal conditions, demonstrating a potential to function as a shelter-vehicle for vaccine delivery. These results suggest that transplastomic plants expressing the rFaeG(ntd/dsc) protein could be used for production and, possibly, delivery of an oral vaccine against porcine F4+ ETEC infections. Our findings therefore present a feasible approach for developing an oral vaccination strategy against porcine PWD.Igor KolotilinAngelo KaldisBert DevriendtJussi JoensuuEric CoxRima MenassaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42405 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Igor Kolotilin Angelo Kaldis Bert Devriendt Jussi Joensuu Eric Cox Rima Menassa Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.
description	Post-weaning diarrhea (PWD) in piglets is a major problem in piggeries worldwide and results in severe economic losses. Infection with Enterotoxigenic Escherichia coli (ETEC) is the key culprit for the PWD disease. F4 fimbriae of ETEC are highly stable proteinaceous polymers, mainly composed of the major structural subunit FaeG, with a capacity to evoke mucosal immune responses, thus demonstrating a potential to act as an oral vaccine against ETEC-induced porcine PWD. In this study we used a transplastomic approach in tobacco to produce a recombinant variant of the FaeG protein, rFaeG(ntd/dsc), engineered for expression as a stable monomer by N-terminal deletion and donor strand-complementation (ntd/dsc). The generated transplastomic tobacco plants accumulated up to 2.0 g rFaeG(ntd/dsc) per 1 kg fresh leaf tissue (more than 1% of dry leaf tissue) and showed normal phenotype indistinguishable from wild type untransformed plants. We determined that chloroplast-produced rFaeG(ntd/dsc) protein retained the key properties of an oral vaccine, i.e. binding to porcine intestinal F4 receptors (F4R), and inhibition of the F4-possessing (F4+) ETEC attachment to F4R. Additionally, the plant biomass matrix was shown to delay degradation of the chloroplast-produced rFaeG(ntd/dsc) in gastrointestinal conditions, demonstrating a potential to function as a shelter-vehicle for vaccine delivery. These results suggest that transplastomic plants expressing the rFaeG(ntd/dsc) protein could be used for production and, possibly, delivery of an oral vaccine against porcine F4+ ETEC infections. Our findings therefore present a feasible approach for developing an oral vaccination strategy against porcine PWD.
format	article
author	Igor Kolotilin Angelo Kaldis Bert Devriendt Jussi Joensuu Eric Cox Rima Menassa
author_facet	Igor Kolotilin Angelo Kaldis Bert Devriendt Jussi Joensuu Eric Cox Rima Menassa
author_sort	Igor Kolotilin
title	Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.
title_short	Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.
title_full	Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.
title_fullStr	Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.
title_full_unstemmed	Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.
title_sort	production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6fdefbeda3d84c26990daac3e2cbbbcb
work_keys_str_mv	AT igorkolotilin productionofasubunitvaccinecandidateagainstporcinepostweaningdiarrheainhighbiomasstransplastomictobacco AT angelokaldis productionofasubunitvaccinecandidateagainstporcinepostweaningdiarrheainhighbiomasstransplastomictobacco AT bertdevriendt productionofasubunitvaccinecandidateagainstporcinepostweaningdiarrheainhighbiomasstransplastomictobacco AT jussijoensuu productionofasubunitvaccinecandidateagainstporcinepostweaningdiarrheainhighbiomasstransplastomictobacco AT ericcox productionofasubunitvaccinecandidateagainstporcinepostweaningdiarrheainhighbiomasstransplastomictobacco AT rimamenassa productionofasubunitvaccinecandidateagainstporcinepostweaningdiarrheainhighbiomasstransplastomictobacco
_version_	1718423857384128512

Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco.

Ejemplares similares