Protective Effects Of Astragalus Polysaccharides On Oxidative Stress In High Glucose-Induced Or SOD2-Silenced H9C2 Cells Based On PCR Array Analysis

Qilin Sun,1,* Xiaoyan Wu,1,* Hao Wang,2 Wenjie Chen,1 Xuelan Zhao,1 Yehong Yang,3 Wei Chen1,* 1Department of Geriatrics, Huashan Hospital, Fudan University, Shanghai 200040, People’s Republic of China; 2Experimental Teaching Center of Basic Medicine, Fudan University, Shanghai 200032, Peop...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sun Q, Wu X, Wang H, Chen W, Zhao X, Yang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Diabetic cardiomyopathy cardiomyocyte oxidative stress ROS PCR Array astragalus polysaccharide Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/6fe3066a984a45f2adc7f351a340ed4e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6fe3066a984a45f2adc7f351a340ed4e
record_format	dspace
spelling	oai:doaj.org-article:6fe3066a984a45f2adc7f351a340ed4e2021-12-02T03:21:56ZProtective Effects Of Astragalus Polysaccharides On Oxidative Stress In High Glucose-Induced Or SOD2-Silenced H9C2 Cells Based On PCR Array Analysis1178-7007https://doaj.org/article/6fe3066a984a45f2adc7f351a340ed4e2019-10-01T00:00:00Zhttps://www.dovepress.com/protective-effects-of-astragalus-polysaccharides-on-oxidative-stress-i-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Qilin Sun,1,* Xiaoyan Wu,1,* Hao Wang,2 Wenjie Chen,1 Xuelan Zhao,1 Yehong Yang,3 Wei Chen1,* 1Department of Geriatrics, Huashan Hospital, Fudan University, Shanghai 200040, People’s Republic of China; 2Experimental Teaching Center of Basic Medicine, Fudan University, Shanghai 200032, People’s Republic of China; 3Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai 200040, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei ChenDepartment of Geriatrics, Huashan Hospital, Fudan University, No.12 Wu-lu-mu-qi Road, Shanghai 200040, People’s Republic of ChinaTel +86 21 5288999ext 7190 Email chenwei_0424@163.comYehong YangDepartment of Endocrinology, Huashan Hospital, Fudan University, No.12 Wu-lu-mu-qi Road, Shanghai 200040, People’s Republic of ChinaTel +86 21 5288999Email yehongyang@126.comBackground: Oxidative stress in cardiac myocytes is an important pathogenesis of diabetic cardiomyopathy (DCM). Previously, we reported that astragalus polysaccharide (APS) has protective effects against the oxidative stress of DCM. This study aimed to determine the effect of APS on the oxidative stress induced by hyperglycemia in H9C2 cells.Methods: Rat H9C2 cells were cultured in vitro and randomly divided into the control group, HG group, APS-HG group, siRNASOD2 group, and APS-siRNASOD2 group. The cellular ultrastructure was measured by transmission electron microscopy. Cell apoptosis was examined by TUNEL staining. Levels of reactive oxygen species (ROS) were detected by a quantitative fluorescence assay (DHE). 8-OH-dG and nitrotyrosine, the indicators of oxidative stress injury, were detected by immunohistochemistry. A PCR array was used to evaluate the expression levels of 84 oxidative stress genes in cultured cells, and the PCR array results were partially verified by Western blot.Results: APS treatment protected the H9C2 cell ultrastructure, reduced the level of cell apoptosis, inhibited cellular ROS production, and reduced the levels of oxidative stress injury indicators 8-OH-dG and nitrotyrosine in high glucose-induced or SOD2-silenced H9C2 cells. It also altered oxidative stress-related genes at the mRNA and protein levels.Conclusion: APS may improve antioxidant capacity and inhibit oxidative stress injury in high glucose induced H9C2 cells.Keywords: diabetic cardiomyopathy, cardiomyocyte, oxidative stress, ROS, PCR array, astragalus polysaccharideSun QWu XWang HChen WZhao XYang YChen WDove Medical PressarticleDiabetic cardiomyopathycardiomyocyteoxidative stressROSPCR Arrayastragalus polysaccharideSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 2209-2220 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diabetic cardiomyopathy cardiomyocyte oxidative stress ROS PCR Array astragalus polysaccharide Specialties of internal medicine RC581-951
spellingShingle	Diabetic cardiomyopathy cardiomyocyte oxidative stress ROS PCR Array astragalus polysaccharide Specialties of internal medicine RC581-951 Sun Q Wu X Wang H Chen W Zhao X Yang Y Chen W Protective Effects Of Astragalus Polysaccharides On Oxidative Stress In High Glucose-Induced Or SOD2-Silenced H9C2 Cells Based On PCR Array Analysis
description	Qilin Sun,1,* Xiaoyan Wu,1,* Hao Wang,2 Wenjie Chen,1 Xuelan Zhao,1 Yehong Yang,3 Wei Chen1,* 1Department of Geriatrics, Huashan Hospital, Fudan University, Shanghai 200040, People’s Republic of China; 2Experimental Teaching Center of Basic Medicine, Fudan University, Shanghai 200032, People’s Republic of China; 3Department of Endocrinology, Huashan Hospital, Fudan University, Shanghai 200040, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei ChenDepartment of Geriatrics, Huashan Hospital, Fudan University, No.12 Wu-lu-mu-qi Road, Shanghai 200040, People’s Republic of ChinaTel +86 21 5288999ext 7190 Email chenwei_0424@163.comYehong YangDepartment of Endocrinology, Huashan Hospital, Fudan University, No.12 Wu-lu-mu-qi Road, Shanghai 200040, People’s Republic of ChinaTel +86 21 5288999Email yehongyang@126.comBackground: Oxidative stress in cardiac myocytes is an important pathogenesis of diabetic cardiomyopathy (DCM). Previously, we reported that astragalus polysaccharide (APS) has protective effects against the oxidative stress of DCM. This study aimed to determine the effect of APS on the oxidative stress induced by hyperglycemia in H9C2 cells.Methods: Rat H9C2 cells were cultured in vitro and randomly divided into the control group, HG group, APS-HG group, siRNASOD2 group, and APS-siRNASOD2 group. The cellular ultrastructure was measured by transmission electron microscopy. Cell apoptosis was examined by TUNEL staining. Levels of reactive oxygen species (ROS) were detected by a quantitative fluorescence assay (DHE). 8-OH-dG and nitrotyrosine, the indicators of oxidative stress injury, were detected by immunohistochemistry. A PCR array was used to evaluate the expression levels of 84 oxidative stress genes in cultured cells, and the PCR array results were partially verified by Western blot.Results: APS treatment protected the H9C2 cell ultrastructure, reduced the level of cell apoptosis, inhibited cellular ROS production, and reduced the levels of oxidative stress injury indicators 8-OH-dG and nitrotyrosine in high glucose-induced or SOD2-silenced H9C2 cells. It also altered oxidative stress-related genes at the mRNA and protein levels.Conclusion: APS may improve antioxidant capacity and inhibit oxidative stress injury in high glucose induced H9C2 cells.Keywords: diabetic cardiomyopathy, cardiomyocyte, oxidative stress, ROS, PCR array, astragalus polysaccharide
format	article
author	Sun Q Wu X Wang H Chen W Zhao X Yang Y Chen W
author_facet	Sun Q Wu X Wang H Chen W Zhao X Yang Y Chen W
author_sort	Sun Q
title	Protective Effects Of Astragalus Polysaccharides On Oxidative Stress In High Glucose-Induced Or SOD2-Silenced H9C2 Cells Based On PCR Array Analysis
title_short	Protective Effects Of Astragalus Polysaccharides On Oxidative Stress In High Glucose-Induced Or SOD2-Silenced H9C2 Cells Based On PCR Array Analysis
title_full	Protective Effects Of Astragalus Polysaccharides On Oxidative Stress In High Glucose-Induced Or SOD2-Silenced H9C2 Cells Based On PCR Array Analysis
title_fullStr	Protective Effects Of Astragalus Polysaccharides On Oxidative Stress In High Glucose-Induced Or SOD2-Silenced H9C2 Cells Based On PCR Array Analysis
title_full_unstemmed	Protective Effects Of Astragalus Polysaccharides On Oxidative Stress In High Glucose-Induced Or SOD2-Silenced H9C2 Cells Based On PCR Array Analysis
title_sort	protective effects of astragalus polysaccharides on oxidative stress in high glucose-induced or sod2-silenced h9c2 cells based on pcr array analysis
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/6fe3066a984a45f2adc7f351a340ed4e
work_keys_str_mv	AT sunq protectiveeffectsofastragaluspolysaccharidesonoxidativestressinhighglucoseinducedorsod2silencedh9c2cellsbasedonpcrarrayanalysis AT wux protectiveeffectsofastragaluspolysaccharidesonoxidativestressinhighglucoseinducedorsod2silencedh9c2cellsbasedonpcrarrayanalysis AT wangh protectiveeffectsofastragaluspolysaccharidesonoxidativestressinhighglucoseinducedorsod2silencedh9c2cellsbasedonpcrarrayanalysis AT chenw protectiveeffectsofastragaluspolysaccharidesonoxidativestressinhighglucoseinducedorsod2silencedh9c2cellsbasedonpcrarrayanalysis AT zhaox protectiveeffectsofastragaluspolysaccharidesonoxidativestressinhighglucoseinducedorsod2silencedh9c2cellsbasedonpcrarrayanalysis AT yangy protectiveeffectsofastragaluspolysaccharidesonoxidativestressinhighglucoseinducedorsod2silencedh9c2cellsbasedonpcrarrayanalysis AT chenw protectiveeffectsofastragaluspolysaccharidesonoxidativestressinhighglucoseinducedorsod2silencedh9c2cellsbasedonpcrarrayanalysis
_version_	1718401797737938944

Protective Effects Of Astragalus Polysaccharides On Oxidative Stress In High Glucose-Induced Or SOD2-Silenced H9C2 Cells Based On PCR Array Analysis

Ejemplares similares