Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection.

<h4>Background</h4>Xenotropic murine leukemia virus-related virus (XMRV) was generated after a recombination event between two endogenous murine leukemia viruses during the production of a prostate cancer cell line. Although the associations of the XMRV infection with human diseases appe...

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Autores principales:	Marta Curriu, Jorge Carrillo, Marta Massanella, Elisabet Garcia, Francesc Cunyat, Ruth Peña, Peter Wienberg, Cristina Carrato, Joan Areal, Margarita Bofill, Bonaventura Clotet, Julià Blanco, Cecilia Cabrera
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6fe572f2355248d8b7317ab3833bbe78
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spelling	oai:doaj.org-article:6fe572f2355248d8b7317ab3833bbe782021-11-18T07:18:26ZSusceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection.1932-620310.1371/journal.pone.0037415https://doaj.org/article/6fe572f2355248d8b7317ab3833bbe782012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22616002/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Xenotropic murine leukemia virus-related virus (XMRV) was generated after a recombination event between two endogenous murine leukemia viruses during the production of a prostate cancer cell line. Although the associations of the XMRV infection with human diseases appear unlikely, the XMRV is a retrovirus of undefined pathogenic potential, able to replicate in human cells in vitro. Since recent studies using animal models for infection have yielded conflicting results, we set out an ex vivo model for XMRV infection of human tonsillar tissue to determine whether XMRV produced by 22Rv1 cells is able to replicate in human lymphoid organs. Tonsil blocks were infected and infection kinetics and its pathogenic effects were monitored<h4>Results</h4>XMRV, though restricted by APOBEC, enters and integrates into the tissue cells. The infection did not result in changes of T or B-cells, immune activation, nor inflammatory chemokines. Infectious viruses could be recovered from supernatants of infected tonsils by reinfecting DERSE XMRV indicator cell line, although these supernatants could not establish a new infection in fresh tonsil culture, indicating that in our model, the viral replication is controlled by innate antiviral restriction factors.<h4>Conclusions</h4>Overall, the replication-competent retrovirus XMRV, present in a high number of laboratories, is able to infect human lymphoid tissue and produce infectious viruses, even though they were unable to establish a new infection in fresh tonsillar tissue. Hereby, laboratories working with cell lines producing XMRV should have knowledge and understanding of the potential biological biohazardous risks of this virus.Marta CurriuJorge CarrilloMarta MassanellaElisabet GarciaFrancesc CunyatRuth PeñaPeter WienbergCristina CarratoJoan ArealMargarita BofillBonaventura ClotetJulià BlancoCecilia CabreraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37415 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta Curriu Jorge Carrillo Marta Massanella Elisabet Garcia Francesc Cunyat Ruth Peña Peter Wienberg Cristina Carrato Joan Areal Margarita Bofill Bonaventura Clotet Julià Blanco Cecilia Cabrera Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection.
description	<h4>Background</h4>Xenotropic murine leukemia virus-related virus (XMRV) was generated after a recombination event between two endogenous murine leukemia viruses during the production of a prostate cancer cell line. Although the associations of the XMRV infection with human diseases appear unlikely, the XMRV is a retrovirus of undefined pathogenic potential, able to replicate in human cells in vitro. Since recent studies using animal models for infection have yielded conflicting results, we set out an ex vivo model for XMRV infection of human tonsillar tissue to determine whether XMRV produced by 22Rv1 cells is able to replicate in human lymphoid organs. Tonsil blocks were infected and infection kinetics and its pathogenic effects were monitored<h4>Results</h4>XMRV, though restricted by APOBEC, enters and integrates into the tissue cells. The infection did not result in changes of T or B-cells, immune activation, nor inflammatory chemokines. Infectious viruses could be recovered from supernatants of infected tonsils by reinfecting DERSE XMRV indicator cell line, although these supernatants could not establish a new infection in fresh tonsil culture, indicating that in our model, the viral replication is controlled by innate antiviral restriction factors.<h4>Conclusions</h4>Overall, the replication-competent retrovirus XMRV, present in a high number of laboratories, is able to infect human lymphoid tissue and produce infectious viruses, even though they were unable to establish a new infection in fresh tonsillar tissue. Hereby, laboratories working with cell lines producing XMRV should have knowledge and understanding of the potential biological biohazardous risks of this virus.
format	article
author	Marta Curriu Jorge Carrillo Marta Massanella Elisabet Garcia Francesc Cunyat Ruth Peña Peter Wienberg Cristina Carrato Joan Areal Margarita Bofill Bonaventura Clotet Julià Blanco Cecilia Cabrera
author_facet	Marta Curriu Jorge Carrillo Marta Massanella Elisabet Garcia Francesc Cunyat Ruth Peña Peter Wienberg Cristina Carrato Joan Areal Margarita Bofill Bonaventura Clotet Julià Blanco Cecilia Cabrera
author_sort	Marta Curriu
title	Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection.
title_short	Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection.
title_full	Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection.
title_fullStr	Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection.
title_full_unstemmed	Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection.
title_sort	susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (xmrv) infection.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6fe572f2355248d8b7317ab3833bbe78
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Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection.

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