Mapping enzyme catalysis with metabolic biosensing

The testing of engineered enzymes represents a bottleneck. Here the authors report a screening method combining microfluidics and mass spectrometry, to map the catalysis of a mutated enzyme, characterise the range of products generated and recover the sequences of variants with desired activities.

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Autores principales:	Linfeng Xu, Kai-Chun Chang, Emory M. Payne, Cyrus Modavi, Leqian Liu, Claire M. Palmer, Nannan Tao, Hal S. Alper, Robert T. Kennedy, Dale S. Cornett, Adam R. Abate
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6fe5bdb2b28d44e59283b3db5ce5e415
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spelling	oai:doaj.org-article:6fe5bdb2b28d44e59283b3db5ce5e4152021-11-28T12:31:02ZMapping enzyme catalysis with metabolic biosensing10.1038/s41467-021-27185-92041-1723https://doaj.org/article/6fe5bdb2b28d44e59283b3db5ce5e4152021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-27185-9https://doaj.org/toc/2041-1723The testing of engineered enzymes represents a bottleneck. Here the authors report a screening method combining microfluidics and mass spectrometry, to map the catalysis of a mutated enzyme, characterise the range of products generated and recover the sequences of variants with desired activities.Linfeng XuKai-Chun ChangEmory M. PayneCyrus ModaviLeqian LiuClaire M. PalmerNannan TaoHal S. AlperRobert T. KennedyDale S. CornettAdam R. AbateNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Linfeng Xu Kai-Chun Chang Emory M. Payne Cyrus Modavi Leqian Liu Claire M. Palmer Nannan Tao Hal S. Alper Robert T. Kennedy Dale S. Cornett Adam R. Abate Mapping enzyme catalysis with metabolic biosensing
description	The testing of engineered enzymes represents a bottleneck. Here the authors report a screening method combining microfluidics and mass spectrometry, to map the catalysis of a mutated enzyme, characterise the range of products generated and recover the sequences of variants with desired activities.
format	article
author	Linfeng Xu Kai-Chun Chang Emory M. Payne Cyrus Modavi Leqian Liu Claire M. Palmer Nannan Tao Hal S. Alper Robert T. Kennedy Dale S. Cornett Adam R. Abate
author_facet	Linfeng Xu Kai-Chun Chang Emory M. Payne Cyrus Modavi Leqian Liu Claire M. Palmer Nannan Tao Hal S. Alper Robert T. Kennedy Dale S. Cornett Adam R. Abate
author_sort	Linfeng Xu
title	Mapping enzyme catalysis with metabolic biosensing
title_short	Mapping enzyme catalysis with metabolic biosensing
title_full	Mapping enzyme catalysis with metabolic biosensing
title_fullStr	Mapping enzyme catalysis with metabolic biosensing
title_full_unstemmed	Mapping enzyme catalysis with metabolic biosensing
title_sort	mapping enzyme catalysis with metabolic biosensing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6fe5bdb2b28d44e59283b3db5ce5e415
work_keys_str_mv	AT linfengxu mappingenzymecatalysiswithmetabolicbiosensing AT kaichunchang mappingenzymecatalysiswithmetabolicbiosensing AT emorympayne mappingenzymecatalysiswithmetabolicbiosensing AT cyrusmodavi mappingenzymecatalysiswithmetabolicbiosensing AT leqianliu mappingenzymecatalysiswithmetabolicbiosensing AT clairempalmer mappingenzymecatalysiswithmetabolicbiosensing AT nannantao mappingenzymecatalysiswithmetabolicbiosensing AT halsalper mappingenzymecatalysiswithmetabolicbiosensing AT roberttkennedy mappingenzymecatalysiswithmetabolicbiosensing AT dalescornett mappingenzymecatalysiswithmetabolicbiosensing AT adamrabate mappingenzymecatalysiswithmetabolicbiosensing
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Mapping enzyme catalysis with metabolic biosensing

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