Mapping enzyme catalysis with metabolic biosensing
The testing of engineered enzymes represents a bottleneck. Here the authors report a screening method combining microfluidics and mass spectrometry, to map the catalysis of a mutated enzyme, characterise the range of products generated and recover the sequences of variants with desired activities.
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Autores principales: | Linfeng Xu, Kai-Chun Chang, Emory M. Payne, Cyrus Modavi, Leqian Liu, Claire M. Palmer, Nannan Tao, Hal S. Alper, Robert T. Kennedy, Dale S. Cornett, Adam R. Abate |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2021
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Materias: | |
Acceso en línea: | https://doaj.org/article/6fe5bdb2b28d44e59283b3db5ce5e415 |
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