Genome-wide prediction of CRISPR/Cas9 targets in Kluyveromyces marxianus and its application to obtain a stable haploid strain
Abstract Kluyveromyces marxianus, a probiotic yeast, is important in industrial applications because it has a broad substrate spectrum, a rapid growth rate and high thermotolerance. To date, however, there has been little effort in its genetic engineering by the CRISPR/Cas9 system. Therefore, we aim...
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Autores principales: | Ming-Hsuan Lee, Jinn-Jy Lin, Yu-Ju Lin, Jui-Jen Chang, Huei-Mien Ke, Wen-Lang Fan, Tzi-Yuan Wang, Wen-Hsiung Li |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2018
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Materias: | |
Acceso en línea: | https://doaj.org/article/6feaf2ff22e74f2eb8d7897cd6ad254d |
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