Genome-wide prediction of CRISPR/Cas9 targets in Kluyveromyces marxianus and its application to obtain a stable haploid strain

Abstract Kluyveromyces marxianus, a probiotic yeast, is important in industrial applications because it has a broad substrate spectrum, a rapid growth rate and high thermotolerance. To date, however, there has been little effort in its genetic engineering by the CRISPR/Cas9 system. Therefore, we aim...

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Autores principales: Ming-Hsuan Lee, Jinn-Jy Lin, Yu-Ju Lin, Jui-Jen Chang, Huei-Mien Ke, Wen-Lang Fan, Tzi-Yuan Wang, Wen-Hsiung Li
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/6feaf2ff22e74f2eb8d7897cd6ad254d
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