Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata

Abstract The desert pioneer plant Stipagrostis pennata plays an important role in sand fixation, wind prevention, and desert ecosystem recovery. An absence of reference genes greatly limits investigations into the regulatory mechanism by which S. pennata adapts to adverse desert environments at the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rong Li, Kaiwen Cui, Quanliang Xie, Shuangquan Xie, Xifeng Chen, Lu Zhuo, Aiping Cao, Haitao Shen, Xiang Jin, Fei Wang, Hongbin Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6febe97e33b443a680bfabf931ed175b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6febe97e33b443a680bfabf931ed175b
record_format	dspace
spelling	oai:doaj.org-article:6febe97e33b443a680bfabf931ed175b2021-11-08T10:52:45ZSelection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata10.1038/s41598-021-00833-22045-2322https://doaj.org/article/6febe97e33b443a680bfabf931ed175b2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00833-2https://doaj.org/toc/2045-2322Abstract The desert pioneer plant Stipagrostis pennata plays an important role in sand fixation, wind prevention, and desert ecosystem recovery. An absence of reference genes greatly limits investigations into the regulatory mechanism by which S. pennata adapts to adverse desert environments at the molecular and genetic levels. In this study, eight candidate reference genes were identified from rhizosheath development transcriptome data from S. pennata, and their expression stability in the rhizosheaths at different development stages, in a variety of plant tissues, and under drought stress was evaluated using four procedures, including geNorm, NormFinder, BestKeeper, and RefFinder. The results showed that GAPDH and elF were the most stable reference genes under drought stress and in rhizosheath development, and ARP6 and ALDH were relatively stable in all plant tissues. In addition, elF was the most suitable reference gene for all treatments. Analysis of the consistency between the reverse transcription-quantitative PCR (RT-qPCR) and RNA sequencing data showed that the identified elF and GAPDH reference genes were stable during rhizosheath development. These results provide reliable reference genes for assuring the accuracy of RT-qPCR and offer a foundation for further investigations into the genetic responses of S. pennata to abiotic stress.Rong LiKaiwen CuiQuanliang XieShuangquan XieXifeng ChenLu ZhuoAiping CaoHaitao ShenXiang JinFei WangHongbin LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rong Li Kaiwen Cui Quanliang Xie Shuangquan Xie Xifeng Chen Lu Zhuo Aiping Cao Haitao Shen Xiang Jin Fei Wang Hongbin Li Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata
description	Abstract The desert pioneer plant Stipagrostis pennata plays an important role in sand fixation, wind prevention, and desert ecosystem recovery. An absence of reference genes greatly limits investigations into the regulatory mechanism by which S. pennata adapts to adverse desert environments at the molecular and genetic levels. In this study, eight candidate reference genes were identified from rhizosheath development transcriptome data from S. pennata, and their expression stability in the rhizosheaths at different development stages, in a variety of plant tissues, and under drought stress was evaluated using four procedures, including geNorm, NormFinder, BestKeeper, and RefFinder. The results showed that GAPDH and elF were the most stable reference genes under drought stress and in rhizosheath development, and ARP6 and ALDH were relatively stable in all plant tissues. In addition, elF was the most suitable reference gene for all treatments. Analysis of the consistency between the reverse transcription-quantitative PCR (RT-qPCR) and RNA sequencing data showed that the identified elF and GAPDH reference genes were stable during rhizosheath development. These results provide reliable reference genes for assuring the accuracy of RT-qPCR and offer a foundation for further investigations into the genetic responses of S. pennata to abiotic stress.
format	article
author	Rong Li Kaiwen Cui Quanliang Xie Shuangquan Xie Xifeng Chen Lu Zhuo Aiping Cao Haitao Shen Xiang Jin Fei Wang Hongbin Li
author_facet	Rong Li Kaiwen Cui Quanliang Xie Shuangquan Xie Xifeng Chen Lu Zhuo Aiping Cao Haitao Shen Xiang Jin Fei Wang Hongbin Li
author_sort	Rong Li
title	Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata
title_short	Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata
title_full	Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata
title_fullStr	Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata
title_full_unstemmed	Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata
title_sort	selection of the reference genes for quantitative gene expression by rt-qpcr in the desert plant stipagrostis pennata
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6febe97e33b443a680bfabf931ed175b
work_keys_str_mv	AT rongli selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata AT kaiwencui selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata AT quanliangxie selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata AT shuangquanxie selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata AT xifengchen selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata AT luzhuo selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata AT aipingcao selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata AT haitaoshen selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata AT xiangjin selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata AT feiwang selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata AT hongbinli selectionofthereferencegenesforquantitativegeneexpressionbyrtqpcrinthedesertplantstipagrostispennata
_version_	1718442536006057984

Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata

Ejemplares similares