Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome

Abstract Neuropeptides are diverse and evolutionarily ancient regulators of physiological/behavioural processes in animals. Here we have investigated the evolution and comparative physiology of luqin-type neuropeptide signalling, which has been characterised previously in protostomian invertebrates....

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Autores principales:	Luis Alfonso Yañez-Guerra, Jérôme Delroisse, Antón Barreiro-Iglesias, Susan E. Slade, James H. Scrivens, Maurice R. Elphick
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ff3d2f780ce4554bdc67b77a6e7fbc2
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spelling	oai:doaj.org-article:6ff3d2f780ce4554bdc67b77a6e7fbc22021-12-02T16:08:27ZDiscovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome10.1038/s41598-018-25606-22045-2322https://doaj.org/article/6ff3d2f780ce4554bdc67b77a6e7fbc22018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25606-2https://doaj.org/toc/2045-2322Abstract Neuropeptides are diverse and evolutionarily ancient regulators of physiological/behavioural processes in animals. Here we have investigated the evolution and comparative physiology of luqin-type neuropeptide signalling, which has been characterised previously in protostomian invertebrates. Phylogenetic analysis indicates that luqin-type receptors and tachykinin-type receptors are paralogous and probably originated in a common ancestor of the Bilateria. In the deuterostomian lineage, luqin-type signalling has been lost in chordates but interestingly it has been retained in ambulacrarians. Therefore, here we characterised luqin-type signalling for the first time in an ambulacrarian – the starfish Asterias rubens (phylum Echinodermata). A luqin-like neuropeptide with a C-terminal RWamide motif (ArLQ; EEKTRFPKFMRW-NH2) was identified as the ligand for two luqin-type receptors in A. rubens, ArLQR1 and ArLQR2. Furthermore, analysis of the expression of the ArLQ precursor using mRNA in situ hybridisation revealed expression in the nervous system, digestive system and locomotory organs (tube feet) and in vitro pharmacology revealed that ArLQ causes dose-dependent relaxation of tube feet. Accordingly, previous studies have revealed that luqin-type signalling regulates feeding and locomotor activity in protostomes. In conclusion, our phylogenetic analysis combined with characterisation of luqin-type signalling in a deuterostome has provided new insights into neuropeptide evolution and function in the animal kingdom.Luis Alfonso Yañez-GuerraJérôme DelroisseAntón Barreiro-IglesiasSusan E. SladeJames H. ScrivensMaurice R. ElphickNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luis Alfonso Yañez-Guerra Jérôme Delroisse Antón Barreiro-Iglesias Susan E. Slade James H. Scrivens Maurice R. Elphick Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome
description	Abstract Neuropeptides are diverse and evolutionarily ancient regulators of physiological/behavioural processes in animals. Here we have investigated the evolution and comparative physiology of luqin-type neuropeptide signalling, which has been characterised previously in protostomian invertebrates. Phylogenetic analysis indicates that luqin-type receptors and tachykinin-type receptors are paralogous and probably originated in a common ancestor of the Bilateria. In the deuterostomian lineage, luqin-type signalling has been lost in chordates but interestingly it has been retained in ambulacrarians. Therefore, here we characterised luqin-type signalling for the first time in an ambulacrarian – the starfish Asterias rubens (phylum Echinodermata). A luqin-like neuropeptide with a C-terminal RWamide motif (ArLQ; EEKTRFPKFMRW-NH2) was identified as the ligand for two luqin-type receptors in A. rubens, ArLQR1 and ArLQR2. Furthermore, analysis of the expression of the ArLQ precursor using mRNA in situ hybridisation revealed expression in the nervous system, digestive system and locomotory organs (tube feet) and in vitro pharmacology revealed that ArLQ causes dose-dependent relaxation of tube feet. Accordingly, previous studies have revealed that luqin-type signalling regulates feeding and locomotor activity in protostomes. In conclusion, our phylogenetic analysis combined with characterisation of luqin-type signalling in a deuterostome has provided new insights into neuropeptide evolution and function in the animal kingdom.
format	article
author	Luis Alfonso Yañez-Guerra Jérôme Delroisse Antón Barreiro-Iglesias Susan E. Slade James H. Scrivens Maurice R. Elphick
author_facet	Luis Alfonso Yañez-Guerra Jérôme Delroisse Antón Barreiro-Iglesias Susan E. Slade James H. Scrivens Maurice R. Elphick
author_sort	Luis Alfonso Yañez-Guerra
title	Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome
title_short	Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome
title_full	Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome
title_fullStr	Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome
title_full_unstemmed	Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome
title_sort	discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6ff3d2f780ce4554bdc67b77a6e7fbc2
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Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome

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