Single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation

Abstract The transfer of mechanical signals through cells is a complex phenomenon. To uncover a new mechanotransduction pathway, we study the frequency-dependent transport of mechanical stimuli by single microtubules and small networks in a bottom-up approach using optically trapped beads as anchor...

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Autores principales:	Matthias D. Koch, Natalie Schneider, Peter Nick, Alexander Rohrbach
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ff609a09ce4425dbf1fc912d19d258a
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spelling	oai:doaj.org-article:6ff609a09ce4425dbf1fc912d19d258a2021-12-02T16:06:30ZSingle microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation10.1038/s41598-017-04415-z2045-2322https://doaj.org/article/6ff609a09ce4425dbf1fc912d19d258a2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04415-zhttps://doaj.org/toc/2045-2322Abstract The transfer of mechanical signals through cells is a complex phenomenon. To uncover a new mechanotransduction pathway, we study the frequency-dependent transport of mechanical stimuli by single microtubules and small networks in a bottom-up approach using optically trapped beads as anchor points. We interconnected microtubules to linear and triangular geometries to perform micro-rheology by defined oscillations of the beads relative to each other. We found a substantial stiffening of single filaments above a characteristic transition frequency of 1–30 Hz depending on the filament’s molecular composition. Below this frequency, filament elasticity only depends on its contour and persistence length. Interestingly, this elastic behavior is transferable to small networks, where we found the surprising effect that linear two filament connections act as transistor-like, angle dependent momentum filters, whereas triangular networks act as stabilizing elements. These observations implicate that cells can tune mechanical signals by temporal and spatial filtering stronger and more flexibly than expected.Matthias D. KochNatalie SchneiderPeter NickAlexander RohrbachNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthias D. Koch Natalie Schneider Peter Nick Alexander Rohrbach Single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation
description	Abstract The transfer of mechanical signals through cells is a complex phenomenon. To uncover a new mechanotransduction pathway, we study the frequency-dependent transport of mechanical stimuli by single microtubules and small networks in a bottom-up approach using optically trapped beads as anchor points. We interconnected microtubules to linear and triangular geometries to perform micro-rheology by defined oscillations of the beads relative to each other. We found a substantial stiffening of single filaments above a characteristic transition frequency of 1–30 Hz depending on the filament’s molecular composition. Below this frequency, filament elasticity only depends on its contour and persistence length. Interestingly, this elastic behavior is transferable to small networks, where we found the surprising effect that linear two filament connections act as transistor-like, angle dependent momentum filters, whereas triangular networks act as stabilizing elements. These observations implicate that cells can tune mechanical signals by temporal and spatial filtering stronger and more flexibly than expected.
format	article
author	Matthias D. Koch Natalie Schneider Peter Nick Alexander Rohrbach
author_facet	Matthias D. Koch Natalie Schneider Peter Nick Alexander Rohrbach
author_sort	Matthias D. Koch
title	Single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation
title_short	Single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation
title_full	Single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation
title_fullStr	Single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation
title_full_unstemmed	Single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation
title_sort	single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6ff609a09ce4425dbf1fc912d19d258a
work_keys_str_mv	AT matthiasdkoch singlemicrotubulesandsmallnetworksbecomesignificantlystifferonshorttimescalesuponmechanicalstimulation AT natalieschneider singlemicrotubulesandsmallnetworksbecomesignificantlystifferonshorttimescalesuponmechanicalstimulation AT peternick singlemicrotubulesandsmallnetworksbecomesignificantlystifferonshorttimescalesuponmechanicalstimulation AT alexanderrohrbach singlemicrotubulesandsmallnetworksbecomesignificantlystifferonshorttimescalesuponmechanicalstimulation
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Single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation

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