A unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach.
Nectarines play a key role in peach industry; the fuzzless skin has implications for consumer acceptance. The peach/nectarine (G/g) trait was described as monogenic and previously mapped on chromosome 5. Here, the position of the G locus was delimited within a 1.1 cM interval (635 kb) based on linka...
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oai:doaj.org-article:6ff772a93ae0455f9c92bb98c20326322021-11-18T08:29:58ZA unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach.1932-620310.1371/journal.pone.0090574https://doaj.org/article/6ff772a93ae0455f9c92bb98c20326322014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24595269/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Nectarines play a key role in peach industry; the fuzzless skin has implications for consumer acceptance. The peach/nectarine (G/g) trait was described as monogenic and previously mapped on chromosome 5. Here, the position of the G locus was delimited within a 1.1 cM interval (635 kb) based on linkage analysis of an F2 progeny from the cross 'Contender' (C, peach) x 'Ambra' (A, nectarine). Careful inspection of the genes annotated in the corresponding genomic sequence (Peach v1.0), coupled with variant discovery, led to the identification of MYB gene PpeMYB25 as a candidate for trichome formation on fruit skin. Analysis of genomic re-sequencing data from five peach/nectarine accessions pointed to the insertion of a LTR retroelement in exon 3 of the PpeMYB25 gene as the cause of the recessive glabrous phenotype. A functional marker (indelG) developed on the LTR insertion cosegregated with the trait in the CxA F2 progeny and was validated on a broad panel of genotypes, including all known putative donors of the nectarine trait. This marker was shown to efficiently discriminate between peach and nectarine plants, indicating that a unique mutational event gave rise to the nectarine trait and providing a useful diagnostic tool for early seedling selection in peach breeding programs.Elisa VendraminGiorgio PeaLuca DondiniIgor PachecoMaria Teresa DettoriLaura GazzaSimone ScalabrinFrancesco StrozziStefano TartariniDaniele BassiIgnazio VerdeLaura RossiniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e90574 (2014) |
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Medicine R Science Q Elisa Vendramin Giorgio Pea Luca Dondini Igor Pacheco Maria Teresa Dettori Laura Gazza Simone Scalabrin Francesco Strozzi Stefano Tartarini Daniele Bassi Ignazio Verde Laura Rossini A unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach. |
| description |
Nectarines play a key role in peach industry; the fuzzless skin has implications for consumer acceptance. The peach/nectarine (G/g) trait was described as monogenic and previously mapped on chromosome 5. Here, the position of the G locus was delimited within a 1.1 cM interval (635 kb) based on linkage analysis of an F2 progeny from the cross 'Contender' (C, peach) x 'Ambra' (A, nectarine). Careful inspection of the genes annotated in the corresponding genomic sequence (Peach v1.0), coupled with variant discovery, led to the identification of MYB gene PpeMYB25 as a candidate for trichome formation on fruit skin. Analysis of genomic re-sequencing data from five peach/nectarine accessions pointed to the insertion of a LTR retroelement in exon 3 of the PpeMYB25 gene as the cause of the recessive glabrous phenotype. A functional marker (indelG) developed on the LTR insertion cosegregated with the trait in the CxA F2 progeny and was validated on a broad panel of genotypes, including all known putative donors of the nectarine trait. This marker was shown to efficiently discriminate between peach and nectarine plants, indicating that a unique mutational event gave rise to the nectarine trait and providing a useful diagnostic tool for early seedling selection in peach breeding programs. |
| format |
article |
| author |
Elisa Vendramin Giorgio Pea Luca Dondini Igor Pacheco Maria Teresa Dettori Laura Gazza Simone Scalabrin Francesco Strozzi Stefano Tartarini Daniele Bassi Ignazio Verde Laura Rossini |
| author_facet |
Elisa Vendramin Giorgio Pea Luca Dondini Igor Pacheco Maria Teresa Dettori Laura Gazza Simone Scalabrin Francesco Strozzi Stefano Tartarini Daniele Bassi Ignazio Verde Laura Rossini |
| author_sort |
Elisa Vendramin |
| title |
A unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach. |
| title_short |
A unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach. |
| title_full |
A unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach. |
| title_fullStr |
A unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach. |
| title_full_unstemmed |
A unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach. |
| title_sort |
unique mutation in a myb gene cosegregates with the nectarine phenotype in peach. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/6ff772a93ae0455f9c92bb98c2032632 |
| work_keys_str_mv |
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