Mapping of histone-binding sites in histone replacement-completed spermatozoa

While a majority of histones are replaced by protamines during spermatogenesis, a small amount is retained in mammalian spermatozoa. Here the authors develop a method to purify histones from replacement-completed sperm (HRCS), completely solubilize histones from cross-linked HRCS without MNase diges...

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Autores principales:	Keisuke Yoshida, Masafumi Muratani, Hiromitsu Araki, Fumihito Miura, Takehiro Suzuki, Naoshi Dohmae, Yuki Katou, Katsuhiko Shirahige, Takashi Ito, Shunsuke Ishii
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6ffb0d6bd687464e8ccf8d9dce3e6ae2
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spelling	oai:doaj.org-article:6ffb0d6bd687464e8ccf8d9dce3e6ae22021-12-02T16:49:56ZMapping of histone-binding sites in histone replacement-completed spermatozoa10.1038/s41467-018-06243-92041-1723https://doaj.org/article/6ffb0d6bd687464e8ccf8d9dce3e6ae22018-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06243-9https://doaj.org/toc/2041-1723While a majority of histones are replaced by protamines during spermatogenesis, a small amount is retained in mammalian spermatozoa. Here the authors develop a method to purify histones from replacement-completed sperm (HRCS), completely solubilize histones from cross-linked HRCS without MNase digestion, and map histone-binding sites in these cells.Keisuke YoshidaMasafumi MurataniHiromitsu ArakiFumihito MiuraTakehiro SuzukiNaoshi DohmaeYuki KatouKatsuhiko ShirahigeTakashi ItoShunsuke IshiiNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Keisuke Yoshida Masafumi Muratani Hiromitsu Araki Fumihito Miura Takehiro Suzuki Naoshi Dohmae Yuki Katou Katsuhiko Shirahige Takashi Ito Shunsuke Ishii Mapping of histone-binding sites in histone replacement-completed spermatozoa
description	While a majority of histones are replaced by protamines during spermatogenesis, a small amount is retained in mammalian spermatozoa. Here the authors develop a method to purify histones from replacement-completed sperm (HRCS), completely solubilize histones from cross-linked HRCS without MNase digestion, and map histone-binding sites in these cells.
format	article
author	Keisuke Yoshida Masafumi Muratani Hiromitsu Araki Fumihito Miura Takehiro Suzuki Naoshi Dohmae Yuki Katou Katsuhiko Shirahige Takashi Ito Shunsuke Ishii
author_facet	Keisuke Yoshida Masafumi Muratani Hiromitsu Araki Fumihito Miura Takehiro Suzuki Naoshi Dohmae Yuki Katou Katsuhiko Shirahige Takashi Ito Shunsuke Ishii
author_sort	Keisuke Yoshida
title	Mapping of histone-binding sites in histone replacement-completed spermatozoa
title_short	Mapping of histone-binding sites in histone replacement-completed spermatozoa
title_full	Mapping of histone-binding sites in histone replacement-completed spermatozoa
title_fullStr	Mapping of histone-binding sites in histone replacement-completed spermatozoa
title_full_unstemmed	Mapping of histone-binding sites in histone replacement-completed spermatozoa
title_sort	mapping of histone-binding sites in histone replacement-completed spermatozoa
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6ffb0d6bd687464e8ccf8d9dce3e6ae2
work_keys_str_mv	AT keisukeyoshida mappingofhistonebindingsitesinhistonereplacementcompletedspermatozoa AT masafumimuratani mappingofhistonebindingsitesinhistonereplacementcompletedspermatozoa AT hiromitsuaraki mappingofhistonebindingsitesinhistonereplacementcompletedspermatozoa AT fumihitomiura mappingofhistonebindingsitesinhistonereplacementcompletedspermatozoa AT takehirosuzuki mappingofhistonebindingsitesinhistonereplacementcompletedspermatozoa AT naoshidohmae mappingofhistonebindingsitesinhistonereplacementcompletedspermatozoa AT yukikatou mappingofhistonebindingsitesinhistonereplacementcompletedspermatozoa AT katsuhikoshirahige mappingofhistonebindingsitesinhistonereplacementcompletedspermatozoa AT takashiito mappingofhistonebindingsitesinhistonereplacementcompletedspermatozoa AT shunsukeishii mappingofhistonebindingsitesinhistonereplacementcompletedspermatozoa
_version_	1718383163936342016

Mapping of histone-binding sites in histone replacement-completed spermatozoa

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