Mapping of histone-binding sites in histone replacement-completed spermatozoa

Mapping of histone-binding sites in histone replacement-completed spermatozoa

While a majority of histones are replaced by protamines during spermatogenesis, a small amount is retained in mammalian spermatozoa. Here the authors develop a method to purify histones from replacement-completed sperm (HRCS), completely solubilize histones from cross-linked HRCS without MNase diges...

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Autores principales: Keisuke Yoshida, Masafumi Muratani, Hiromitsu Araki, Fumihito Miura, Takehiro Suzuki, Naoshi Dohmae, Yuki Katou, Katsuhiko Shirahige, Takashi Ito, Shunsuke Ishii
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
Science
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Acceso en línea:https://doaj.org/article/6ffb0d6bd687464e8ccf8d9dce3e6ae2
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