Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways

Abstract Among its other biological roles, acetylcholinesterase (AChE, EC 3.1.1.7), encoded by two ace in most insects, catalyses the breakdown of acetylcholine, thereby terminating synaptic transmission. ace1 encodes the synaptic enzyme and ace2 has other essential actions in many insect species, s...

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Autores principales:	Xinhai Ye, Liwen Yang, David Stanley, Fei Li, Qi Fang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ffde492e08c495889edb33de45711b5
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spelling	oai:doaj.org-article:6ffde492e08c495889edb33de45711b52021-12-02T16:08:09ZTwo Bombyx mori acetylcholinesterase genes influence motor control and development in different ways10.1038/s41598-017-05360-72045-2322https://doaj.org/article/6ffde492e08c495889edb33de45711b52017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05360-7https://doaj.org/toc/2045-2322Abstract Among its other biological roles, acetylcholinesterase (AChE, EC 3.1.1.7), encoded by two ace in most insects, catalyses the breakdown of acetylcholine, thereby terminating synaptic transmission. ace1 encodes the synaptic enzyme and ace2 has other essential actions in many insect species, such as Chilo suppressalis and Plutella xylostella. The silkworm, Bombyx mori, has been domesticated for more than two thousand years and its aces have no history of pesticide exposure. Here, we investigated the functional differences between two ace genes, BmAce1 and BmAce2, in the silkworm. qPCR analysis indicated that BmAce1 is highly expressed in muscle and BmAce2 is more ubiquitously expressed among tissues and enriched in the head. Both genes were separately suppressed using chemically synthesized siRNAs. The mRNA abundance of the two ace genes was significantly reduced to about 13% – 75% of the control levels after siRNA injection. The AChE activities were decreased to 32% to 85% of control levels. Silencing BmAce2 resulted in about 26% mortality, faster and higher than the 20% in the siBmAce1-treated group. Silencing BmAce1 impacted motor control and development to a greater extent than silencing BmAce2, although both treatment groups suffered motor disability, slowed development and reduced cocoons. Both genes have essential, differing biological significance.Xinhai YeLiwen YangDavid StanleyFei LiQi FangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xinhai Ye Liwen Yang David Stanley Fei Li Qi Fang Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways
description	Abstract Among its other biological roles, acetylcholinesterase (AChE, EC 3.1.1.7), encoded by two ace in most insects, catalyses the breakdown of acetylcholine, thereby terminating synaptic transmission. ace1 encodes the synaptic enzyme and ace2 has other essential actions in many insect species, such as Chilo suppressalis and Plutella xylostella. The silkworm, Bombyx mori, has been domesticated for more than two thousand years and its aces have no history of pesticide exposure. Here, we investigated the functional differences between two ace genes, BmAce1 and BmAce2, in the silkworm. qPCR analysis indicated that BmAce1 is highly expressed in muscle and BmAce2 is more ubiquitously expressed among tissues and enriched in the head. Both genes were separately suppressed using chemically synthesized siRNAs. The mRNA abundance of the two ace genes was significantly reduced to about 13% – 75% of the control levels after siRNA injection. The AChE activities were decreased to 32% to 85% of control levels. Silencing BmAce2 resulted in about 26% mortality, faster and higher than the 20% in the siBmAce1-treated group. Silencing BmAce1 impacted motor control and development to a greater extent than silencing BmAce2, although both treatment groups suffered motor disability, slowed development and reduced cocoons. Both genes have essential, differing biological significance.
format	article
author	Xinhai Ye Liwen Yang David Stanley Fei Li Qi Fang
author_facet	Xinhai Ye Liwen Yang David Stanley Fei Li Qi Fang
author_sort	Xinhai Ye
title	Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways
title_short	Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways
title_full	Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways
title_fullStr	Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways
title_full_unstemmed	Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways
title_sort	two bombyx mori acetylcholinesterase genes influence motor control and development in different ways
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6ffde492e08c495889edb33de45711b5
work_keys_str_mv	AT xinhaiye twobombyxmoriacetylcholinesterasegenesinfluencemotorcontrolanddevelopmentindifferentways AT liwenyang twobombyxmoriacetylcholinesterasegenesinfluencemotorcontrolanddevelopmentindifferentways AT davidstanley twobombyxmoriacetylcholinesterasegenesinfluencemotorcontrolanddevelopmentindifferentways AT feili twobombyxmoriacetylcholinesterasegenesinfluencemotorcontrolanddevelopmentindifferentways AT qifang twobombyxmoriacetylcholinesterasegenesinfluencemotorcontrolanddevelopmentindifferentways
_version_	1718384603184496640

Two Bombyx mori acetylcholinesterase genes influence motor control and development in different ways

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