Loss of toll-like receptor 4 ameliorates cardiovascular dysfunction in aged mice

Abstract Background Toll-like receptor 4 (TLR4) is a pattern recognition receptor of the innate immune system. TLR4 contributes to many aging-related chronic diseases. However, whether TLR4 is involved in cardiovascular injury during the aging process has not been investigated. Methods The effects o...

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Autores principales:	Huan Liu, Shujuan Chu, Zhilin Wu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Immunologic diseases. Allergy RC581-607 Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/700215ff57ac4d46a1344f17a9b3f126
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spelling	oai:doaj.org-article:700215ff57ac4d46a1344f17a9b3f1262021-11-07T12:12:16ZLoss of toll-like receptor 4 ameliorates cardiovascular dysfunction in aged mice10.1186/s12979-021-00251-y1742-4933https://doaj.org/article/700215ff57ac4d46a1344f17a9b3f1262021-11-01T00:00:00Zhttps://doi.org/10.1186/s12979-021-00251-yhttps://doaj.org/toc/1742-4933Abstract Background Toll-like receptor 4 (TLR4) is a pattern recognition receptor of the innate immune system. TLR4 contributes to many aging-related chronic diseases. However, whether TLR4 is involved in cardiovascular injury during the aging process has not been investigated. Methods The effects of TLR4 on the cardiovascular system of aged mice were investigated in TLR4−/− mice. An intraperitoneal glucose tolerance test (IPGTT) and insulin sensitivity test (IST) were conducted to evaluate global insulin sensitivity. Echocardiography was used to measure cardiac structure and performance. An isolated artery ring assay was used to measure the vasodilator function of the thoracic aorta. The inflammatory response was reflected by the serum concentration of cytokines. Results TLR4 expression increased in the hearts and aortas of mice in an age-dependent manner. Loss of TLR4 increased insulin sensitivity in aged mice. Moreover, loss of TLR4 improved cardiac performance and endothelium-dependent vascular relaxation in aged mice. Importantly, the increases in serum inflammatory cytokines and oxidative stress in the heart and aorta were also inhibited by TLR4 deficiency. Conclusion In summary, loss of TLR4 improved cardiac performance and endothelium-dependent vascular relaxation in aged mice. The reduced inflammatory responses and oxidative stress may be the reason for the protective effects of TLR4 deficiency during aging. Our study indicates that targeting TLR4 is a potential therapeutic strategy for preventing aging-related cardiovascular disease.Huan LiuShujuan ChuZhilin WuBMCarticleImmunologic diseases. AllergyRC581-607GeriatricsRC952-954.6ENImmunity & Ageing, Vol 18, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Geriatrics RC952-954.6
spellingShingle	Immunologic diseases. Allergy RC581-607 Geriatrics RC952-954.6 Huan Liu Shujuan Chu Zhilin Wu Loss of toll-like receptor 4 ameliorates cardiovascular dysfunction in aged mice
description	Abstract Background Toll-like receptor 4 (TLR4) is a pattern recognition receptor of the innate immune system. TLR4 contributes to many aging-related chronic diseases. However, whether TLR4 is involved in cardiovascular injury during the aging process has not been investigated. Methods The effects of TLR4 on the cardiovascular system of aged mice were investigated in TLR4−/− mice. An intraperitoneal glucose tolerance test (IPGTT) and insulin sensitivity test (IST) were conducted to evaluate global insulin sensitivity. Echocardiography was used to measure cardiac structure and performance. An isolated artery ring assay was used to measure the vasodilator function of the thoracic aorta. The inflammatory response was reflected by the serum concentration of cytokines. Results TLR4 expression increased in the hearts and aortas of mice in an age-dependent manner. Loss of TLR4 increased insulin sensitivity in aged mice. Moreover, loss of TLR4 improved cardiac performance and endothelium-dependent vascular relaxation in aged mice. Importantly, the increases in serum inflammatory cytokines and oxidative stress in the heart and aorta were also inhibited by TLR4 deficiency. Conclusion In summary, loss of TLR4 improved cardiac performance and endothelium-dependent vascular relaxation in aged mice. The reduced inflammatory responses and oxidative stress may be the reason for the protective effects of TLR4 deficiency during aging. Our study indicates that targeting TLR4 is a potential therapeutic strategy for preventing aging-related cardiovascular disease.
format	article
author	Huan Liu Shujuan Chu Zhilin Wu
author_facet	Huan Liu Shujuan Chu Zhilin Wu
author_sort	Huan Liu
title	Loss of toll-like receptor 4 ameliorates cardiovascular dysfunction in aged mice
title_short	Loss of toll-like receptor 4 ameliorates cardiovascular dysfunction in aged mice
title_full	Loss of toll-like receptor 4 ameliorates cardiovascular dysfunction in aged mice
title_fullStr	Loss of toll-like receptor 4 ameliorates cardiovascular dysfunction in aged mice
title_full_unstemmed	Loss of toll-like receptor 4 ameliorates cardiovascular dysfunction in aged mice
title_sort	loss of toll-like receptor 4 ameliorates cardiovascular dysfunction in aged mice
publisher	BMC
publishDate	2021
url	https://doaj.org/article/700215ff57ac4d46a1344f17a9b3f126
work_keys_str_mv	AT huanliu lossoftolllikereceptor4amelioratescardiovasculardysfunctioninagedmice AT shujuanchu lossoftolllikereceptor4amelioratescardiovasculardysfunctioninagedmice AT zhilinwu lossoftolllikereceptor4amelioratescardiovasculardysfunctioninagedmice
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Loss of toll-like receptor 4 ameliorates cardiovascular dysfunction in aged mice

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