Effects of RNAi-mediated plasma membrane calcium transporting ATPase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of Mythimna separata to wilforine

Wilforine, a compound of sesquiterpene alkaloids isolated from Tripterygium wilfordii, exhibits excellent insecticidal activity against Mythimna separata. In order to clarify the action mechanism of wilforine, the plasma membrane calcium transporting ATPase (PMCA) and inositol 1,4,5-trisphosphate re...

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Autores principales:	Xiao-peng Lu, Jia-huan Liu, Xiang-yun Fu, Feng-jin Wang, Hua Wu, Hua Weng, Zhi-qing Ma
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	PMCA IP3R Botanical pesticides Wilforine Mythimna separata Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/70044537513d4772bdfe0fae7820301a
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spelling	oai:doaj.org-article:70044537513d4772bdfe0fae7820301a2021-11-06T04:16:44ZEffects of RNAi-mediated plasma membrane calcium transporting ATPase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of Mythimna separata to wilforine0147-651310.1016/j.ecoenv.2021.112909https://doaj.org/article/70044537513d4772bdfe0fae7820301a2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010216https://doaj.org/toc/0147-6513Wilforine, a compound of sesquiterpene alkaloids isolated from Tripterygium wilfordii, exhibits excellent insecticidal activity against Mythimna separata. In order to clarify the action mechanism of wilforine, the plasma membrane calcium transporting ATPase (PMCA) and inositol 1,4,5-trisphosphate receptor (IP3R) from M. separata were studied. Results showed that the open reading frame of MsIP3R and MsPMCA were 8118 bp and 3438 bp in length, as well as encoded 2706 and 1146 amino acids, respectively. Multiple sequence alignment and phylogenetic analysis revealed that the MsIP3R and MsPMCA had high homology with the IP3R and PMCA of other insects, but had low similarity with those of mammals, which means the IP3R and PMCA have potential to be the novel targets of insecticides with high selectivity between mammals and insects. Both MsIP3R and MsPMCA genes existed throughout the life cycle of M. separata, and were all predominantly expressed in somatic muscle of fifth-instar larvae and the adults. The susceptibilities of PMCA-silenced M. separata to wilforine were significantly lower than that of the normal M. separata, which illustrates that PMCA could be one of the targets of wilforine. However, the susceptibilities of IP3R-silenced M. separata to wilforine did not change significantly compared with the susceptibilities of normal M. separata, which shows that wilforine may not interact with the IP3R protein. These findings provide clues for elucidating the insecticidal mechanism of wilforine.Xiao-peng LuJia-huan LiuXiang-yun FuFeng-jin WangHua WuHua WengZhi-qing MaElsevierarticlePMCAIP3RBotanical pesticidesWilforineMythimna separataEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112909- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PMCA IP3R Botanical pesticides Wilforine Mythimna separata Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	PMCA IP3R Botanical pesticides Wilforine Mythimna separata Environmental pollution TD172-193.5 Environmental sciences GE1-350 Xiao-peng Lu Jia-huan Liu Xiang-yun Fu Feng-jin Wang Hua Wu Hua Weng Zhi-qing Ma Effects of RNAi-mediated plasma membrane calcium transporting ATPase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of Mythimna separata to wilforine
description	Wilforine, a compound of sesquiterpene alkaloids isolated from Tripterygium wilfordii, exhibits excellent insecticidal activity against Mythimna separata. In order to clarify the action mechanism of wilforine, the plasma membrane calcium transporting ATPase (PMCA) and inositol 1,4,5-trisphosphate receptor (IP3R) from M. separata were studied. Results showed that the open reading frame of MsIP3R and MsPMCA were 8118 bp and 3438 bp in length, as well as encoded 2706 and 1146 amino acids, respectively. Multiple sequence alignment and phylogenetic analysis revealed that the MsIP3R and MsPMCA had high homology with the IP3R and PMCA of other insects, but had low similarity with those of mammals, which means the IP3R and PMCA have potential to be the novel targets of insecticides with high selectivity between mammals and insects. Both MsIP3R and MsPMCA genes existed throughout the life cycle of M. separata, and were all predominantly expressed in somatic muscle of fifth-instar larvae and the adults. The susceptibilities of PMCA-silenced M. separata to wilforine were significantly lower than that of the normal M. separata, which illustrates that PMCA could be one of the targets of wilforine. However, the susceptibilities of IP3R-silenced M. separata to wilforine did not change significantly compared with the susceptibilities of normal M. separata, which shows that wilforine may not interact with the IP3R protein. These findings provide clues for elucidating the insecticidal mechanism of wilforine.
format	article
author	Xiao-peng Lu Jia-huan Liu Xiang-yun Fu Feng-jin Wang Hua Wu Hua Weng Zhi-qing Ma
author_facet	Xiao-peng Lu Jia-huan Liu Xiang-yun Fu Feng-jin Wang Hua Wu Hua Weng Zhi-qing Ma
author_sort	Xiao-peng Lu
title	Effects of RNAi-mediated plasma membrane calcium transporting ATPase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of Mythimna separata to wilforine
title_short	Effects of RNAi-mediated plasma membrane calcium transporting ATPase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of Mythimna separata to wilforine
title_full	Effects of RNAi-mediated plasma membrane calcium transporting ATPase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of Mythimna separata to wilforine
title_fullStr	Effects of RNAi-mediated plasma membrane calcium transporting ATPase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of Mythimna separata to wilforine
title_full_unstemmed	Effects of RNAi-mediated plasma membrane calcium transporting ATPase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of Mythimna separata to wilforine
title_sort	effects of rnai-mediated plasma membrane calcium transporting atpase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of mythimna separata to wilforine
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/70044537513d4772bdfe0fae7820301a
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Effects of RNAi-mediated plasma membrane calcium transporting ATPase and inositol 1,4,5-trisphosphate receptor gene silencing on the susceptibility of Mythimna separata to wilforine

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