Biodegradation and Micro- Scale Treatability Pattern of Loblolly Pine Heartwood Bioincised by Bacillus Subtilis and Physisporinus Vitreus

One strategy for improving the treatability of refractory wood species is biological incising, and its efficiency depends on how the microorganisms modify the porous structure of the wood. Evaluation of the bioincised wood treatability on a micro-scale can thus help to better understand the treatab...

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Autores principales:	Ismaeil Zahedi Tajrishi, Asghar Tarmian, Reza Oladi, Miha Humar, Masoud Ahmadzadeh
Formato:	article
Lenguaje:	EN
Publicado:	University of Zagreb, Faculty of Forestry and Wood Technology 2021
Materias:	bacillus subtilis bioincising penetration physisporinus vitreus wood treatability Forestry SD1-669.5
Acceso en línea:	https://doaj.org/article/7006fe6e0b6a4f33bce83d46f1d71ab0
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spelling	oai:doaj.org-article:7006fe6e0b6a4f33bce83d46f1d71ab02021-11-26T09:43:42ZBiodegradation and Micro- Scale Treatability Pattern of Loblolly Pine Heartwood Bioincised by Bacillus Subtilis and Physisporinus Vitreus0012-67721847-115310.5552/drvind.2021.2034https://doaj.org/article/7006fe6e0b6a4f33bce83d46f1d71ab02021-01-01T00:00:00Zhttps://hrcak.srce.hr/file/385830https://doaj.org/toc/0012-6772https://doaj.org/toc/1847-1153One strategy for improving the treatability of refractory wood species is biological incising, and its efficiency depends on how the microorganisms modify the porous structure of the wood. Evaluation of the bioincised wood treatability on a micro-scale can thus help to better understand the treatability enhancing mechanisms. In the present study, the biodegradation pattern and micro-scale treatability of Loblolly pine (Pinus taeda L.) heartwood were determined after bioincising with the white-rot fungus Physisporinus vitreus (Pers.: Fr.) P. Karsten isolate 136 and bacterium Bacillus subtilis UTB22. Oven-dried specimens with dimensions of 50 mm × 25 mm × 15 mm (L × T × R) were incubated with the microorganisms at (23±2) °C and (65±5) % relative humidity for six weeks. The control and exposed wood blocks were then pressure treated by 1 % fluorescent dye (fluorescein)-containing water to study the treatability pattern under a fluorescence microscope. The longitudinal and tangential air permeability and compression strength parallel to the grain of the specimens were also determined at the end of the incubation period. Scanning electron microscopic (SEM) studies showed that degradation by B. subtilis UTB22 was limited to the pit membranes, but the cell walls were also degraded to some extent by P. vitreus. The fungus caused a higher mass loss compared to the bacterium, whereas the permeability enhancing ability of the bacterium was more pronounced. The fluorescent dye tracer also showed that higher treatability with more uniformity was obtained by B. subtilis UTB22. The improvement in treatability by both microorganisms was mainly due to the degradation of the earlywood tracheids.Ismaeil Zahedi TajrishiAsghar TarmianReza OladiMiha HumarMasoud AhmadzadehUniversity of Zagreb, Faculty of Forestry and Wood Technologyarticlebacillus subtilisbioincisingpenetrationphysisporinus vitreuswood treatabilityForestrySD1-669.5ENDrvna Industrija, Vol 72, Iss 4, Pp 365-372 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacillus subtilis bioincising penetration physisporinus vitreus wood treatability Forestry SD1-669.5
spellingShingle	bacillus subtilis bioincising penetration physisporinus vitreus wood treatability Forestry SD1-669.5 Ismaeil Zahedi Tajrishi Asghar Tarmian Reza Oladi Miha Humar Masoud Ahmadzadeh Biodegradation and Micro- Scale Treatability Pattern of Loblolly Pine Heartwood Bioincised by Bacillus Subtilis and Physisporinus Vitreus
description	One strategy for improving the treatability of refractory wood species is biological incising, and its efficiency depends on how the microorganisms modify the porous structure of the wood. Evaluation of the bioincised wood treatability on a micro-scale can thus help to better understand the treatability enhancing mechanisms. In the present study, the biodegradation pattern and micro-scale treatability of Loblolly pine (Pinus taeda L.) heartwood were determined after bioincising with the white-rot fungus Physisporinus vitreus (Pers.: Fr.) P. Karsten isolate 136 and bacterium Bacillus subtilis UTB22. Oven-dried specimens with dimensions of 50 mm × 25 mm × 15 mm (L × T × R) were incubated with the microorganisms at (23±2) °C and (65±5) % relative humidity for six weeks. The control and exposed wood blocks were then pressure treated by 1 % fluorescent dye (fluorescein)-containing water to study the treatability pattern under a fluorescence microscope. The longitudinal and tangential air permeability and compression strength parallel to the grain of the specimens were also determined at the end of the incubation period. Scanning electron microscopic (SEM) studies showed that degradation by B. subtilis UTB22 was limited to the pit membranes, but the cell walls were also degraded to some extent by P. vitreus. The fungus caused a higher mass loss compared to the bacterium, whereas the permeability enhancing ability of the bacterium was more pronounced. The fluorescent dye tracer also showed that higher treatability with more uniformity was obtained by B. subtilis UTB22. The improvement in treatability by both microorganisms was mainly due to the degradation of the earlywood tracheids.
format	article
author	Ismaeil Zahedi Tajrishi Asghar Tarmian Reza Oladi Miha Humar Masoud Ahmadzadeh
author_facet	Ismaeil Zahedi Tajrishi Asghar Tarmian Reza Oladi Miha Humar Masoud Ahmadzadeh
author_sort	Ismaeil Zahedi Tajrishi
title	Biodegradation and Micro- Scale Treatability Pattern of Loblolly Pine Heartwood Bioincised by Bacillus Subtilis and Physisporinus Vitreus
title_short	Biodegradation and Micro- Scale Treatability Pattern of Loblolly Pine Heartwood Bioincised by Bacillus Subtilis and Physisporinus Vitreus
title_full	Biodegradation and Micro- Scale Treatability Pattern of Loblolly Pine Heartwood Bioincised by Bacillus Subtilis and Physisporinus Vitreus
title_fullStr	Biodegradation and Micro- Scale Treatability Pattern of Loblolly Pine Heartwood Bioincised by Bacillus Subtilis and Physisporinus Vitreus
title_full_unstemmed	Biodegradation and Micro- Scale Treatability Pattern of Loblolly Pine Heartwood Bioincised by Bacillus Subtilis and Physisporinus Vitreus
title_sort	biodegradation and micro- scale treatability pattern of loblolly pine heartwood bioincised by bacillus subtilis and physisporinus vitreus
publisher	University of Zagreb, Faculty of Forestry and Wood Technology
publishDate	2021
url	https://doaj.org/article/7006fe6e0b6a4f33bce83d46f1d71ab0
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Biodegradation and Micro- Scale Treatability Pattern of Loblolly Pine Heartwood Bioincised by Bacillus Subtilis and Physisporinus Vitreus

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