Integrated microRNA and mRNA signatures associated with overall survival in epithelial ovarian cancer.

Ovarian cancer (OC), the eighth-leading cause of cancer-related death among females worldwide, is mainly represented by epithelial OC (EOC) that can be further subdivided into four subtypes: serous (75%), endometrioid (10%), clear cell (10%), and mucinous (3%). Major reasons for high mortality are t...

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Autores principales:	Joanna Lopacinska-Jørgensen, Douglas V N P Oliveira, Guy Wayne Novotny, Claus K Høgdall, Estrid V Høgdall
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/700bdd88a24441b4a0594e9266e649d5
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spelling	oai:doaj.org-article:700bdd88a24441b4a0594e9266e649d52021-12-02T20:09:01ZIntegrated microRNA and mRNA signatures associated with overall survival in epithelial ovarian cancer.1932-620310.1371/journal.pone.0255142https://doaj.org/article/700bdd88a24441b4a0594e9266e649d52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255142https://doaj.org/toc/1932-6203Ovarian cancer (OC), the eighth-leading cause of cancer-related death among females worldwide, is mainly represented by epithelial OC (EOC) that can be further subdivided into four subtypes: serous (75%), endometrioid (10%), clear cell (10%), and mucinous (3%). Major reasons for high mortality are the poor biological understanding of the OC mechanisms and a lack of reliable markers defining each EOC subtype. MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression primarily by targeting messenger RNA (mRNA) transcripts. Their aberrant expression patterns have been associated with cancer development, including OC. However, the role of miRNAs in tumorigenesis is still to be determined, mainly due to the lack of consensus regarding optimal methodologies for identification and validation of miRNAs and their targets. Several tools for computational target prediction exist, but false interpretations remain a problem. The experimental validation of every potential miRNA-mRNA pair is not feasible, as it is laborious and expensive. In this study, we analyzed the correlation between global miRNA and mRNA expression patterns derived from microarray profiling of 197 EOC patients to identify the signatures of miRNA-mRNA interactions associated with overall survival (OS). The aim was to investigate whether these miRNA-mRNA signatures might have a prognostic value for OS in different subtypes of EOC. The content of our cohort (162 serous carcinomas, 15 endometrioid carcinomas, 11 mucinous carcinomas, and 9 clear cell carcinomas) reflects a real-world scenario of EOC. Several interaction pairs between 6 miRNAs (hsa-miR-126-3p, hsa-miR-223-3p, hsa-miR-23a-5p, hsa-miR-27a-5p, hsa-miR-486-5p, and hsa-miR-506-3p) and 8 mRNAs (ATF3, CH25H, EMP1, HBB, HBEGF, NAMPT, POSTN, and PROCR) were identified and the findings appear to be well supported by the literature. This indicates that our study has a potential to reveal miRNA-mRNA signatures relevant for EOC. Thus, the evaluation on independent cohorts will further evaluate the performance of such findings.Joanna Lopacinska-JørgensenDouglas V N P OliveiraGuy Wayne NovotnyClaus K HøgdallEstrid V HøgdallPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255142 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joanna Lopacinska-Jørgensen Douglas V N P Oliveira Guy Wayne Novotny Claus K Høgdall Estrid V Høgdall Integrated microRNA and mRNA signatures associated with overall survival in epithelial ovarian cancer.
description	Ovarian cancer (OC), the eighth-leading cause of cancer-related death among females worldwide, is mainly represented by epithelial OC (EOC) that can be further subdivided into four subtypes: serous (75%), endometrioid (10%), clear cell (10%), and mucinous (3%). Major reasons for high mortality are the poor biological understanding of the OC mechanisms and a lack of reliable markers defining each EOC subtype. MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression primarily by targeting messenger RNA (mRNA) transcripts. Their aberrant expression patterns have been associated with cancer development, including OC. However, the role of miRNAs in tumorigenesis is still to be determined, mainly due to the lack of consensus regarding optimal methodologies for identification and validation of miRNAs and their targets. Several tools for computational target prediction exist, but false interpretations remain a problem. The experimental validation of every potential miRNA-mRNA pair is not feasible, as it is laborious and expensive. In this study, we analyzed the correlation between global miRNA and mRNA expression patterns derived from microarray profiling of 197 EOC patients to identify the signatures of miRNA-mRNA interactions associated with overall survival (OS). The aim was to investigate whether these miRNA-mRNA signatures might have a prognostic value for OS in different subtypes of EOC. The content of our cohort (162 serous carcinomas, 15 endometrioid carcinomas, 11 mucinous carcinomas, and 9 clear cell carcinomas) reflects a real-world scenario of EOC. Several interaction pairs between 6 miRNAs (hsa-miR-126-3p, hsa-miR-223-3p, hsa-miR-23a-5p, hsa-miR-27a-5p, hsa-miR-486-5p, and hsa-miR-506-3p) and 8 mRNAs (ATF3, CH25H, EMP1, HBB, HBEGF, NAMPT, POSTN, and PROCR) were identified and the findings appear to be well supported by the literature. This indicates that our study has a potential to reveal miRNA-mRNA signatures relevant for EOC. Thus, the evaluation on independent cohorts will further evaluate the performance of such findings.
format	article
author	Joanna Lopacinska-Jørgensen Douglas V N P Oliveira Guy Wayne Novotny Claus K Høgdall Estrid V Høgdall
author_facet	Joanna Lopacinska-Jørgensen Douglas V N P Oliveira Guy Wayne Novotny Claus K Høgdall Estrid V Høgdall
author_sort	Joanna Lopacinska-Jørgensen
title	Integrated microRNA and mRNA signatures associated with overall survival in epithelial ovarian cancer.
title_short	Integrated microRNA and mRNA signatures associated with overall survival in epithelial ovarian cancer.
title_full	Integrated microRNA and mRNA signatures associated with overall survival in epithelial ovarian cancer.
title_fullStr	Integrated microRNA and mRNA signatures associated with overall survival in epithelial ovarian cancer.
title_full_unstemmed	Integrated microRNA and mRNA signatures associated with overall survival in epithelial ovarian cancer.
title_sort	integrated microrna and mrna signatures associated with overall survival in epithelial ovarian cancer.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/700bdd88a24441b4a0594e9266e649d5
work_keys_str_mv	AT joannalopacinskajørgensen integratedmicrornaandmrnasignaturesassociatedwithoverallsurvivalinepithelialovariancancer AT douglasvnpoliveira integratedmicrornaandmrnasignaturesassociatedwithoverallsurvivalinepithelialovariancancer AT guywaynenovotny integratedmicrornaandmrnasignaturesassociatedwithoverallsurvivalinepithelialovariancancer AT clauskhøgdall integratedmicrornaandmrnasignaturesassociatedwithoverallsurvivalinepithelialovariancancer AT estridvhøgdall integratedmicrornaandmrnasignaturesassociatedwithoverallsurvivalinepithelialovariancancer
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Integrated microRNA and mRNA signatures associated with overall survival in epithelial ovarian cancer.

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