Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance

Glutathione-s-transferase is believed to be involved in the resistance to chemotherapeutic drugs, which depends on the interaction with the cell membranes. In this study, we employed Langmuir monolayers of a mixture of phospholipids and cholesterol (MIX) as models for tumor cell membranes and invest...

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Autores principales:	Elsa M. Materón, Flavio M. Shimizu, Kevin Figueiredo dos Santos, Gustavo F. Nascimento, Vananélia P.N. Geraldo, Osvaldo N. Oliveira Jr, Ronaldo C. Faria
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Membrane model Langmuir monolayer Cisplatin Doxorubicin Detoxification enzyme Glutathione-s-transferase Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/701448312fe243b2a86a9e1461e926e1
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spelling	oai:doaj.org-article:701448312fe243b2a86a9e1461e926e12021-12-02T04:59:06ZMembrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance0753-332210.1016/j.biopha.2021.112426https://doaj.org/article/701448312fe243b2a86a9e1461e926e12022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221012129https://doaj.org/toc/0753-3322Glutathione-s-transferase is believed to be involved in the resistance to chemotherapeutic drugs, which depends on the interaction with the cell membranes. In this study, we employed Langmuir monolayers of a mixture of phospholipids and cholesterol (MIX) as models for tumor cell membranes and investigated their interaction with the anticancer drugs cisplatin (CDDP) and doxorubicin (DOX). We found that both DOX and CDDP expand and affect the elasticity of MIX monolayers, but these effects are hindered when glutathione-s-transferase (GST) and its cofactor glutathione (GSH) are incorporated. Changes are induced by DOX or CDDP on the polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) data for MIX/GST/GSH monolayers, thus denoting some degree of interaction that is not sufficient to alter the monolayer mechanical properties. Overall, the results presented here give support to the hypothesis of the inactivation of DOX and CDDP by GST and point to possible directions to detect and fight drug resistance.Elsa M. MaterónFlavio M. ShimizuKevin Figueiredo dos SantosGustavo F. NascimentoVananélia P.N. GeraldoOsvaldo N. Oliveira JrRonaldo C. FariaElsevierarticleMembrane modelLangmuir monolayerCisplatinDoxorubicinDetoxification enzymeGlutathione-s-transferaseTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112426- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Membrane model Langmuir monolayer Cisplatin Doxorubicin Detoxification enzyme Glutathione-s-transferase Therapeutics. Pharmacology RM1-950
spellingShingle	Membrane model Langmuir monolayer Cisplatin Doxorubicin Detoxification enzyme Glutathione-s-transferase Therapeutics. Pharmacology RM1-950 Elsa M. Materón Flavio M. Shimizu Kevin Figueiredo dos Santos Gustavo F. Nascimento Vananélia P.N. Geraldo Osvaldo N. Oliveira Jr Ronaldo C. Faria Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
description	Glutathione-s-transferase is believed to be involved in the resistance to chemotherapeutic drugs, which depends on the interaction with the cell membranes. In this study, we employed Langmuir monolayers of a mixture of phospholipids and cholesterol (MIX) as models for tumor cell membranes and investigated their interaction with the anticancer drugs cisplatin (CDDP) and doxorubicin (DOX). We found that both DOX and CDDP expand and affect the elasticity of MIX monolayers, but these effects are hindered when glutathione-s-transferase (GST) and its cofactor glutathione (GSH) are incorporated. Changes are induced by DOX or CDDP on the polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) data for MIX/GST/GSH monolayers, thus denoting some degree of interaction that is not sufficient to alter the monolayer mechanical properties. Overall, the results presented here give support to the hypothesis of the inactivation of DOX and CDDP by GST and point to possible directions to detect and fight drug resistance.
format	article
author	Elsa M. Materón Flavio M. Shimizu Kevin Figueiredo dos Santos Gustavo F. Nascimento Vananélia P.N. Geraldo Osvaldo N. Oliveira Jr Ronaldo C. Faria
author_facet	Elsa M. Materón Flavio M. Shimizu Kevin Figueiredo dos Santos Gustavo F. Nascimento Vananélia P.N. Geraldo Osvaldo N. Oliveira Jr Ronaldo C. Faria
author_sort	Elsa M. Materón
title	Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
title_short	Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
title_full	Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
title_fullStr	Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
title_full_unstemmed	Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
title_sort	membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/701448312fe243b2a86a9e1461e926e1
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Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance

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