Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance

Glutathione-s-transferase is believed to be involved in the resistance to chemotherapeutic drugs, which depends on the interaction with the cell membranes. In this study, we employed Langmuir monolayers of a mixture of phospholipids and cholesterol (MIX) as models for tumor cell membranes and invest...

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Autores principales: Elsa M. Materón, Flavio M. Shimizu, Kevin Figueiredo dos Santos, Gustavo F. Nascimento, Vananélia P.N. Geraldo, Osvaldo N. Oliveira Jr, Ronaldo C. Faria
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Publicado: Elsevier 2022
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Acceso en línea:https://doaj.org/article/701448312fe243b2a86a9e1461e926e1
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spelling oai:doaj.org-article:701448312fe243b2a86a9e1461e926e12021-12-02T04:59:06ZMembrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance0753-332210.1016/j.biopha.2021.112426https://doaj.org/article/701448312fe243b2a86a9e1461e926e12022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221012129https://doaj.org/toc/0753-3322Glutathione-s-transferase is believed to be involved in the resistance to chemotherapeutic drugs, which depends on the interaction with the cell membranes. In this study, we employed Langmuir monolayers of a mixture of phospholipids and cholesterol (MIX) as models for tumor cell membranes and investigated their interaction with the anticancer drugs cisplatin (CDDP) and doxorubicin (DOX). We found that both DOX and CDDP expand and affect the elasticity of MIX monolayers, but these effects are hindered when glutathione-s-transferase (GST) and its cofactor glutathione (GSH) are incorporated. Changes are induced by DOX or CDDP on the polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) data for MIX/GST/GSH monolayers, thus denoting some degree of interaction that is not sufficient to alter the monolayer mechanical properties. Overall, the results presented here give support to the hypothesis of the inactivation of DOX and CDDP by GST and point to possible directions to detect and fight drug resistance.Elsa M. MaterónFlavio M. ShimizuKevin Figueiredo dos SantosGustavo F. NascimentoVananélia P.N. GeraldoOsvaldo N. Oliveira JrRonaldo C. FariaElsevierarticleMembrane modelLangmuir monolayerCisplatinDoxorubicinDetoxification enzymeGlutathione-s-transferaseTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112426- (2022)
institution DOAJ
collection DOAJ
language EN
topic Membrane model
Langmuir monolayer
Cisplatin
Doxorubicin
Detoxification enzyme
Glutathione-s-transferase
Therapeutics. Pharmacology
RM1-950
spellingShingle Membrane model
Langmuir monolayer
Cisplatin
Doxorubicin
Detoxification enzyme
Glutathione-s-transferase
Therapeutics. Pharmacology
RM1-950
Elsa M. Materón
Flavio M. Shimizu
Kevin Figueiredo dos Santos
Gustavo F. Nascimento
Vananélia P.N. Geraldo
Osvaldo N. Oliveira Jr
Ronaldo C. Faria
Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
description Glutathione-s-transferase is believed to be involved in the resistance to chemotherapeutic drugs, which depends on the interaction with the cell membranes. In this study, we employed Langmuir monolayers of a mixture of phospholipids and cholesterol (MIX) as models for tumor cell membranes and investigated their interaction with the anticancer drugs cisplatin (CDDP) and doxorubicin (DOX). We found that both DOX and CDDP expand and affect the elasticity of MIX monolayers, but these effects are hindered when glutathione-s-transferase (GST) and its cofactor glutathione (GSH) are incorporated. Changes are induced by DOX or CDDP on the polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) data for MIX/GST/GSH monolayers, thus denoting some degree of interaction that is not sufficient to alter the monolayer mechanical properties. Overall, the results presented here give support to the hypothesis of the inactivation of DOX and CDDP by GST and point to possible directions to detect and fight drug resistance.
format article
author Elsa M. Materón
Flavio M. Shimizu
Kevin Figueiredo dos Santos
Gustavo F. Nascimento
Vananélia P.N. Geraldo
Osvaldo N. Oliveira Jr
Ronaldo C. Faria
author_facet Elsa M. Materón
Flavio M. Shimizu
Kevin Figueiredo dos Santos
Gustavo F. Nascimento
Vananélia P.N. Geraldo
Osvaldo N. Oliveira Jr
Ronaldo C. Faria
author_sort Elsa M. Materón
title Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
title_short Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
title_full Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
title_fullStr Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
title_full_unstemmed Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
title_sort membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance
publisher Elsevier
publishDate 2022
url https://doaj.org/article/701448312fe243b2a86a9e1461e926e1
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