Circulating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer

Abstract Circulating exosome holds great potentials as biomarker for diagnosis and prognosis of human cancers. Previously, we have applied small RNA sequencing to identify aberrantly expressed exosomal miRNAs as candidates for diagnostic markers in colon cancer patients. In this validation cohort, p...

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Autores principales:	Jing Wang, Feihu Yan, Qi Zhao, Fei Zhan, Ruitao Wang, Liang Wang, Yanqiao Zhang, Xiaoyi Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7015d7cbd6064f7bae1094328e840c29
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spelling	oai:doaj.org-article:7015d7cbd6064f7bae1094328e840c292021-12-02T12:30:24ZCirculating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer10.1038/s41598-017-04386-12045-2322https://doaj.org/article/7015d7cbd6064f7bae1094328e840c292017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04386-1https://doaj.org/toc/2045-2322Abstract Circulating exosome holds great potentials as biomarker for diagnosis and prognosis of human cancers. Previously, we have applied small RNA sequencing to identify aberrantly expressed exosomal miRNAs as candidates for diagnostic markers in colon cancer patients. In this validation cohort, plasma derived exosomal miRNA was isolated from 50 early-stage colon cancer patients and 50 matched healthy volunteers. Real-time qRT-PCR revealed that miR-125a-3p, miR-320c were significantly up-regulated in plasma exosomes of the patients with early stage colon cancer. ROC curve showed that miR-125a-3p abundant level may predict colon cancer with an area of under the curve (AUC) of 68.5%, in comparison to that of CEA at 83.6%. Combination of miR-125a-3P and CEA improved the AUC to 85.5%. In addition, plasma exosome level of miR-125a-3p and miR-320c showed significant correlation with nerve infiltration (P < 0.01), but not with tumor size, infiltration depth, and differentiation degree (P > 0.05). On the contrary, plasma CEA level is correlated with tumor size, infiltration depth, and differentiation degree (P < 0.05, r = 0.3009–0.7270), but not with nerve infiltration (P = 0.744). In conclusion, this follow-up study demonstrated circulating plasma exosomal miR-125a-3p is readily accessible as diagnosis biomarker for early-stage colon cancer. When combined with conventional diagnostic markers, miR-125a-3p can improve the diagnostic power.Jing WangFeihu YanQi ZhaoFei ZhanRuitao WangLiang WangYanqiao ZhangXiaoyi HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Wang Feihu Yan Qi Zhao Fei Zhan Ruitao Wang Liang Wang Yanqiao Zhang Xiaoyi Huang Circulating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer
description	Abstract Circulating exosome holds great potentials as biomarker for diagnosis and prognosis of human cancers. Previously, we have applied small RNA sequencing to identify aberrantly expressed exosomal miRNAs as candidates for diagnostic markers in colon cancer patients. In this validation cohort, plasma derived exosomal miRNA was isolated from 50 early-stage colon cancer patients and 50 matched healthy volunteers. Real-time qRT-PCR revealed that miR-125a-3p, miR-320c were significantly up-regulated in plasma exosomes of the patients with early stage colon cancer. ROC curve showed that miR-125a-3p abundant level may predict colon cancer with an area of under the curve (AUC) of 68.5%, in comparison to that of CEA at 83.6%. Combination of miR-125a-3P and CEA improved the AUC to 85.5%. In addition, plasma exosome level of miR-125a-3p and miR-320c showed significant correlation with nerve infiltration (P < 0.01), but not with tumor size, infiltration depth, and differentiation degree (P > 0.05). On the contrary, plasma CEA level is correlated with tumor size, infiltration depth, and differentiation degree (P < 0.05, r = 0.3009–0.7270), but not with nerve infiltration (P = 0.744). In conclusion, this follow-up study demonstrated circulating plasma exosomal miR-125a-3p is readily accessible as diagnosis biomarker for early-stage colon cancer. When combined with conventional diagnostic markers, miR-125a-3p can improve the diagnostic power.
format	article
author	Jing Wang Feihu Yan Qi Zhao Fei Zhan Ruitao Wang Liang Wang Yanqiao Zhang Xiaoyi Huang
author_facet	Jing Wang Feihu Yan Qi Zhao Fei Zhan Ruitao Wang Liang Wang Yanqiao Zhang Xiaoyi Huang
author_sort	Jing Wang
title	Circulating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer
title_short	Circulating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer
title_full	Circulating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer
title_fullStr	Circulating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer
title_full_unstemmed	Circulating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer
title_sort	circulating exosomal mir-125a-3p as a novel biomarker for early-stage colon cancer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7015d7cbd6064f7bae1094328e840c29
work_keys_str_mv	AT jingwang circulatingexosomalmir125a3pasanovelbiomarkerforearlystagecoloncancer AT feihuyan circulatingexosomalmir125a3pasanovelbiomarkerforearlystagecoloncancer AT qizhao circulatingexosomalmir125a3pasanovelbiomarkerforearlystagecoloncancer AT feizhan circulatingexosomalmir125a3pasanovelbiomarkerforearlystagecoloncancer AT ruitaowang circulatingexosomalmir125a3pasanovelbiomarkerforearlystagecoloncancer AT liangwang circulatingexosomalmir125a3pasanovelbiomarkerforearlystagecoloncancer AT yanqiaozhang circulatingexosomalmir125a3pasanovelbiomarkerforearlystagecoloncancer AT xiaoyihuang circulatingexosomalmir125a3pasanovelbiomarkerforearlystagecoloncancer
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Circulating exosomal miR-125a-3p as a novel biomarker for early-stage colon cancer

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