Detecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging

Abstract Targeted therapies specific to the BRAF-MEK-ERK signaling pathway have shown great promise in the treatment of malignant melanoma in the last few years, with these drugs now commonly used in clinic. Melanoma cells treated using these agents are known to exhibit increased levels of melanin p...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anant Shah, Teresa Delgado-Goni, Teresa Casals Galobart, Slawomir Wantuch, Yann Jamin, Martin O. Leach, Simon P. Robinson, Jeffrey Bamber, Mounia Beloueche-Babari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/70160c2970da4f94b357818550a07a16
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:70160c2970da4f94b357818550a07a16
record_format	dspace
spelling	oai:doaj.org-article:70160c2970da4f94b357818550a07a162021-12-02T15:05:12ZDetecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging10.1038/s41598-017-07864-82045-2322https://doaj.org/article/70160c2970da4f94b357818550a07a162017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07864-8https://doaj.org/toc/2045-2322Abstract Targeted therapies specific to the BRAF-MEK-ERK signaling pathway have shown great promise in the treatment of malignant melanoma in the last few years, with these drugs now commonly used in clinic. Melanoma cells treated using these agents are known to exhibit increased levels of melanin pigment and tyrosinase activity. In this study we assessed the potential of non-invasive imaging approaches (photoacoustic imaging (PAI) and magnetic resonance imaging (MRI)) to detect melanin induction in SKMEL28 human melanoma cells, following inhibition of Hsp90 and BRAF signaling using 17-AAG and vemurafenib, respectively. We confirmed, using western blot and spectrophotometry, that Hsp90 or BRAF inhibitor-induced melanoma cell differentiation resulted in an upregulation of tyrosinase and melanin expression levels, in comparison to control cells. This post-treatment increase in cellular pigmentation induced a significant increase in PAI signals that are spectrally identifiable and shortening of the MRI relaxation times T 1 and $${{\boldsymbol{T}}}_{{\bf{2}}}^{{\boldsymbol{\ast }}}$$ T 2 ∗ . This proof-of-concept study demonstrates the potential of MRI and PAI for detecting the downstream cellular changes induced by Hsp90 and BRAF-MEK-targeted therapies in melanoma cells with potential significance for in vivo imaging.Anant ShahTeresa Delgado-GoniTeresa Casals GalobartSlawomir WantuchYann JaminMartin O. LeachSimon P. RobinsonJeffrey BamberMounia Beloueche-BabariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anant Shah Teresa Delgado-Goni Teresa Casals Galobart Slawomir Wantuch Yann Jamin Martin O. Leach Simon P. Robinson Jeffrey Bamber Mounia Beloueche-Babari Detecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging
description	Abstract Targeted therapies specific to the BRAF-MEK-ERK signaling pathway have shown great promise in the treatment of malignant melanoma in the last few years, with these drugs now commonly used in clinic. Melanoma cells treated using these agents are known to exhibit increased levels of melanin pigment and tyrosinase activity. In this study we assessed the potential of non-invasive imaging approaches (photoacoustic imaging (PAI) and magnetic resonance imaging (MRI)) to detect melanin induction in SKMEL28 human melanoma cells, following inhibition of Hsp90 and BRAF signaling using 17-AAG and vemurafenib, respectively. We confirmed, using western blot and spectrophotometry, that Hsp90 or BRAF inhibitor-induced melanoma cell differentiation resulted in an upregulation of tyrosinase and melanin expression levels, in comparison to control cells. This post-treatment increase in cellular pigmentation induced a significant increase in PAI signals that are spectrally identifiable and shortening of the MRI relaxation times T 1 and $${{\boldsymbol{T}}}_{{\bf{2}}}^{{\boldsymbol{\ast }}}$$ T 2 ∗ . This proof-of-concept study demonstrates the potential of MRI and PAI for detecting the downstream cellular changes induced by Hsp90 and BRAF-MEK-targeted therapies in melanoma cells with potential significance for in vivo imaging.
format	article
author	Anant Shah Teresa Delgado-Goni Teresa Casals Galobart Slawomir Wantuch Yann Jamin Martin O. Leach Simon P. Robinson Jeffrey Bamber Mounia Beloueche-Babari
author_facet	Anant Shah Teresa Delgado-Goni Teresa Casals Galobart Slawomir Wantuch Yann Jamin Martin O. Leach Simon P. Robinson Jeffrey Bamber Mounia Beloueche-Babari
author_sort	Anant Shah
title	Detecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging
title_short	Detecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging
title_full	Detecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging
title_fullStr	Detecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging
title_full_unstemmed	Detecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging
title_sort	detecting human melanoma cell re-differentiation following braf or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/70160c2970da4f94b357818550a07a16
work_keys_str_mv	AT anantshah detectinghumanmelanomacellredifferentiationfollowingbraforheatshockprotein90inhibitionusingphotoacousticandmagneticresonanceimaging AT teresadelgadogoni detectinghumanmelanomacellredifferentiationfollowingbraforheatshockprotein90inhibitionusingphotoacousticandmagneticresonanceimaging AT teresacasalsgalobart detectinghumanmelanomacellredifferentiationfollowingbraforheatshockprotein90inhibitionusingphotoacousticandmagneticresonanceimaging AT slawomirwantuch detectinghumanmelanomacellredifferentiationfollowingbraforheatshockprotein90inhibitionusingphotoacousticandmagneticresonanceimaging AT yannjamin detectinghumanmelanomacellredifferentiationfollowingbraforheatshockprotein90inhibitionusingphotoacousticandmagneticresonanceimaging AT martinoleach detectinghumanmelanomacellredifferentiationfollowingbraforheatshockprotein90inhibitionusingphotoacousticandmagneticresonanceimaging AT simonprobinson detectinghumanmelanomacellredifferentiationfollowingbraforheatshockprotein90inhibitionusingphotoacousticandmagneticresonanceimaging AT jeffreybamber detectinghumanmelanomacellredifferentiationfollowingbraforheatshockprotein90inhibitionusingphotoacousticandmagneticresonanceimaging AT mouniabelouechebabari detectinghumanmelanomacellredifferentiationfollowingbraforheatshockprotein90inhibitionusingphotoacousticandmagneticresonanceimaging
_version_	1718388896022134784

Detecting human melanoma cell re-differentiation following BRAF or heat shock protein 90 inhibition using photoacoustic and magnetic resonance imaging

Ejemplares similares