Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration
Background: Regeneration of fertile plants from tissue culture is a critical bottleneck in the application of new plant breeding technologies. Ectopic overexpression of morphogenic factors is a promising workaround for this hurdle. Methods: Conditional overexpression of <i>WUS</i> and &l...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/70179e5e631e4696a2008b4a54559e49 |
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| Sumario: | Background: Regeneration of fertile plants from tissue culture is a critical bottleneck in the application of new plant breeding technologies. Ectopic overexpression of morphogenic factors is a promising workaround for this hurdle. Methods: Conditional overexpression of <i>WUS</i> and <i>ARF5</i>Δ was used to study the effect of timing the overexpression of these morphogenic factors during shoot regeneration from root explants in Arabidopsis. In addition, their effect on auxin-signaling activation was examined by visualization and cytometric quantification of the DR5:GFP auxin-signaling reporter in roots and protoplasts, respectively. Results: The induced expression of both <i>WUS</i> and <i>ARF5</i>Δ led to an activation of auxin signaling in roots. Activation of auxin signaling by <i>WUS</i> and <i>ARF5</i>Δ was further quantified by transient transformation of protoplasts. Ectopic overexpression of both <i>WUS</i> and <i>ARF5</i>Δ enhanced regeneration efficiency, but only during the shoot-induction stage of regeneration and not during the callus-induction stage. Conclusions: The overexpression of <i>WUS</i> and <i>ARF5</i>Δ both lead to activation of auxin signaling. Expression during the shoot-induction stage is critical for the enhancement of shoot regeneration by <i>WUS</i> and <i>ARF5</i>Δ. |
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