Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration

Background: Regeneration of fertile plants from tissue culture is a critical bottleneck in the application of new plant breeding technologies. Ectopic overexpression of morphogenic factors is a promising workaround for this hurdle. Methods: Conditional overexpression of <i>WUS</i> and &l...

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Autores principales:	Juan H. Gonzalez, Joseph S. Taylor, Kelsey M. Reed, R. Clay Wright, Bastiaan O. R. Bargmann
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plant regeneration morphogenic factors conditional expression auxin signaling Botany QK1-989
Acceso en línea:	https://doaj.org/article/70179e5e631e4696a2008b4a54559e49
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spelling	oai:doaj.org-article:70179e5e631e4696a2008b4a54559e492021-11-25T18:44:33ZTemporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration10.3390/plants101122712223-7747https://doaj.org/article/70179e5e631e4696a2008b4a54559e492021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2271https://doaj.org/toc/2223-7747Background: Regeneration of fertile plants from tissue culture is a critical bottleneck in the application of new plant breeding technologies. Ectopic overexpression of morphogenic factors is a promising workaround for this hurdle. Methods: Conditional overexpression of <i>WUS</i> and <i>ARF5</i>Δ was used to study the effect of timing the overexpression of these morphogenic factors during shoot regeneration from root explants in Arabidopsis. In addition, their effect on auxin-signaling activation was examined by visualization and cytometric quantification of the DR5:GFP auxin-signaling reporter in roots and protoplasts, respectively. Results: The induced expression of both <i>WUS</i> and <i>ARF5</i>Δ led to an activation of auxin signaling in roots. Activation of auxin signaling by <i>WUS</i> and <i>ARF5</i>Δ was further quantified by transient transformation of protoplasts. Ectopic overexpression of both <i>WUS</i> and <i>ARF5</i>Δ enhanced regeneration efficiency, but only during the shoot-induction stage of regeneration and not during the callus-induction stage. Conclusions: The overexpression of <i>WUS</i> and <i>ARF5</i>Δ both lead to activation of auxin signaling. Expression during the shoot-induction stage is critical for the enhancement of shoot regeneration by <i>WUS</i> and <i>ARF5</i>Δ.Juan H. GonzalezJoseph S. TaylorKelsey M. ReedR. Clay WrightBastiaan O. R. BargmannMDPI AGarticleplant regenerationmorphogenic factorsconditional expressionauxin signalingBotanyQK1-989ENPlants, Vol 10, Iss 2271, p 2271 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant regeneration morphogenic factors conditional expression auxin signaling Botany QK1-989
spellingShingle	plant regeneration morphogenic factors conditional expression auxin signaling Botany QK1-989 Juan H. Gonzalez Joseph S. Taylor Kelsey M. Reed R. Clay Wright Bastiaan O. R. Bargmann Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration
description	Background: Regeneration of fertile plants from tissue culture is a critical bottleneck in the application of new plant breeding technologies. Ectopic overexpression of morphogenic factors is a promising workaround for this hurdle. Methods: Conditional overexpression of <i>WUS</i> and <i>ARF5</i>Δ was used to study the effect of timing the overexpression of these morphogenic factors during shoot regeneration from root explants in Arabidopsis. In addition, their effect on auxin-signaling activation was examined by visualization and cytometric quantification of the DR5:GFP auxin-signaling reporter in roots and protoplasts, respectively. Results: The induced expression of both <i>WUS</i> and <i>ARF5</i>Δ led to an activation of auxin signaling in roots. Activation of auxin signaling by <i>WUS</i> and <i>ARF5</i>Δ was further quantified by transient transformation of protoplasts. Ectopic overexpression of both <i>WUS</i> and <i>ARF5</i>Δ enhanced regeneration efficiency, but only during the shoot-induction stage of regeneration and not during the callus-induction stage. Conclusions: The overexpression of <i>WUS</i> and <i>ARF5</i>Δ both lead to activation of auxin signaling. Expression during the shoot-induction stage is critical for the enhancement of shoot regeneration by <i>WUS</i> and <i>ARF5</i>Δ.
format	article
author	Juan H. Gonzalez Joseph S. Taylor Kelsey M. Reed R. Clay Wright Bastiaan O. R. Bargmann
author_facet	Juan H. Gonzalez Joseph S. Taylor Kelsey M. Reed R. Clay Wright Bastiaan O. R. Bargmann
author_sort	Juan H. Gonzalez
title	Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration
title_short	Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration
title_full	Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration
title_fullStr	Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration
title_full_unstemmed	Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration
title_sort	temporal control of morphogenic factor expression determines efficacy in enhancing regeneration
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/70179e5e631e4696a2008b4a54559e49
work_keys_str_mv	AT juanhgonzalez temporalcontrolofmorphogenicfactorexpressiondeterminesefficacyinenhancingregeneration AT josephstaylor temporalcontrolofmorphogenicfactorexpressiondeterminesefficacyinenhancingregeneration AT kelseymreed temporalcontrolofmorphogenicfactorexpressiondeterminesefficacyinenhancingregeneration AT rclaywright temporalcontrolofmorphogenicfactorexpressiondeterminesefficacyinenhancingregeneration AT bastiaanorbargmann temporalcontrolofmorphogenicfactorexpressiondeterminesefficacyinenhancingregeneration
_version_	1718410779283161088

Temporal Control of Morphogenic Factor Expression Determines Efficacy in Enhancing Regeneration

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