Ion channels alterations in the forebrain of high-fat diet fed rats

Evidence suggests that transient receptor potential (TRP) ion channels dysfunction significantly contributes to the physiopathology of metabolic and neurological disorders. Dysregulation in functions and expression in genes encoding the TRP channels cause several inherited diseases in humans (the so...

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Autores principales:	Proshanta Roy, Ilenia Martinelli, Michele Moruzzi, Federica Maggi, Consuelo Amantini, Maria Vittoria Micioni Di Bonaventura, Carlo Cifani, Francesco Amenta, Seyed Khosrow Tayebati, Daniele Tomassoni
Formato:	article
Lenguaje:	EN
Publicado:	PAGEPress Publications 2021
Materias:	TRP Channel Brain alteration Obesity High-fat diet Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7017a438516f45f3a722c23da259afcd
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spelling	oai:doaj.org-article:7017a438516f45f3a722c23da259afcd2021-11-24T07:51:42ZIon channels alterations in the forebrain of high-fat diet fed rats10.4081/ejh.2021.33051121-760X2038-8306https://doaj.org/article/7017a438516f45f3a722c23da259afcd2021-11-01T00:00:00Zhttps://ejh.it/index.php/ejh/article/view/3305https://doaj.org/toc/1121-760Xhttps://doaj.org/toc/2038-8306Evidence suggests that transient receptor potential (TRP) ion channels dysfunction significantly contributes to the physiopathology of metabolic and neurological disorders. Dysregulation in functions and expression in genes encoding the TRP channels cause several inherited diseases in humans (the so-called ‘TRP channelopathies’), which affect the cardiovascular, renal, skeletal, and nervous systems. This study aimed to evaluate the expression of ion channels in the forebrain of rats with diet-induced obesity (DIO). DIO rats were studied after 17 weeks under a hypercaloric diet (high-fat diet, HFD) and were compared to the control rats with a standard diet (CHOW). To determine the systemic effects of HFD exposure, we examined food intake, fat mass content, fasting glycemia, insulin levels, cholesterol, and triglycerides. qRT-PCR, Western blot, and immunochemistry analysis were performed in the frontal cortex (FC) and hippocampus (HIP). After 17 weeks of HFD, DIO rats increased their body weight significantly compared to the CHOW rats. In DIO rats, TRPC1 and TRPC6 were upregulated in the HIP, while they were downregulated in the FC. In the case of TRPM2 expression, instead was increased both in the HIP and in the FC. These could be related to the increase of proteins and nucleic acid oxidation. TRPV1 and TRPV2 gene expression showed no differences both in the FC and HIP. In general, qRT-PCR analyses were confirmed by Western blot analysis. Immunohistochemical procedures highlighted the expression of the channels in the cell body of neurons and axons, particularly for the TRPC1 and TRPC6. The alterations of TRP channel expression could be related to the activation of glial cells or the neurodegenerative process presented in the brain of the DIO rat highlighted with post synaptic protein (PSD 95) alterations. The availability of suitable animal models may be useful for studying possible pharmacological treatments to counter obesity-induced brain injury. The identified changes in DIO rats may represent the first insight to characterize the neuronal alterations occurring in obesity. Further investigations are necessary to characterize the role of TRP channels in the regulation of synaptic plasticity and obesity-related cognitive decline. Proshanta RoyIlenia MartinelliMichele MoruzziFederica MaggiConsuelo AmantiniMaria Vittoria Micioni Di BonaventuraCarlo CifaniFrancesco AmentaSeyed Khosrow TayebatiDaniele TomassoniPAGEPress PublicationsarticleTRP ChannelBrain alterationObesityHigh-fat dietBiology (General)QH301-705.5ENEuropean Journal of Histochemistry , Vol 65, Iss s1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TRP Channel Brain alteration Obesity High-fat diet Biology (General) QH301-705.5
spellingShingle	TRP Channel Brain alteration Obesity High-fat diet Biology (General) QH301-705.5 Proshanta Roy Ilenia Martinelli Michele Moruzzi Federica Maggi Consuelo Amantini Maria Vittoria Micioni Di Bonaventura Carlo Cifani Francesco Amenta Seyed Khosrow Tayebati Daniele Tomassoni Ion channels alterations in the forebrain of high-fat diet fed rats
description	Evidence suggests that transient receptor potential (TRP) ion channels dysfunction significantly contributes to the physiopathology of metabolic and neurological disorders. Dysregulation in functions and expression in genes encoding the TRP channels cause several inherited diseases in humans (the so-called ‘TRP channelopathies’), which affect the cardiovascular, renal, skeletal, and nervous systems. This study aimed to evaluate the expression of ion channels in the forebrain of rats with diet-induced obesity (DIO). DIO rats were studied after 17 weeks under a hypercaloric diet (high-fat diet, HFD) and were compared to the control rats with a standard diet (CHOW). To determine the systemic effects of HFD exposure, we examined food intake, fat mass content, fasting glycemia, insulin levels, cholesterol, and triglycerides. qRT-PCR, Western blot, and immunochemistry analysis were performed in the frontal cortex (FC) and hippocampus (HIP). After 17 weeks of HFD, DIO rats increased their body weight significantly compared to the CHOW rats. In DIO rats, TRPC1 and TRPC6 were upregulated in the HIP, while they were downregulated in the FC. In the case of TRPM2 expression, instead was increased both in the HIP and in the FC. These could be related to the increase of proteins and nucleic acid oxidation. TRPV1 and TRPV2 gene expression showed no differences both in the FC and HIP. In general, qRT-PCR analyses were confirmed by Western blot analysis. Immunohistochemical procedures highlighted the expression of the channels in the cell body of neurons and axons, particularly for the TRPC1 and TRPC6. The alterations of TRP channel expression could be related to the activation of glial cells or the neurodegenerative process presented in the brain of the DIO rat highlighted with post synaptic protein (PSD 95) alterations. The availability of suitable animal models may be useful for studying possible pharmacological treatments to counter obesity-induced brain injury. The identified changes in DIO rats may represent the first insight to characterize the neuronal alterations occurring in obesity. Further investigations are necessary to characterize the role of TRP channels in the regulation of synaptic plasticity and obesity-related cognitive decline.
format	article
author	Proshanta Roy Ilenia Martinelli Michele Moruzzi Federica Maggi Consuelo Amantini Maria Vittoria Micioni Di Bonaventura Carlo Cifani Francesco Amenta Seyed Khosrow Tayebati Daniele Tomassoni
author_facet	Proshanta Roy Ilenia Martinelli Michele Moruzzi Federica Maggi Consuelo Amantini Maria Vittoria Micioni Di Bonaventura Carlo Cifani Francesco Amenta Seyed Khosrow Tayebati Daniele Tomassoni
author_sort	Proshanta Roy
title	Ion channels alterations in the forebrain of high-fat diet fed rats
title_short	Ion channels alterations in the forebrain of high-fat diet fed rats
title_full	Ion channels alterations in the forebrain of high-fat diet fed rats
title_fullStr	Ion channels alterations in the forebrain of high-fat diet fed rats
title_full_unstemmed	Ion channels alterations in the forebrain of high-fat diet fed rats
title_sort	ion channels alterations in the forebrain of high-fat diet fed rats
publisher	PAGEPress Publications
publishDate	2021
url	https://doaj.org/article/7017a438516f45f3a722c23da259afcd
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Ion channels alterations in the forebrain of high-fat diet fed rats

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