Identification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis

Abstract It has been suggested that menstrual blood-derived mesenchymal stem/stromal cells (MenMSCs) are associated with the etiopathogenesis of endometriosis and considerable effort has been invested in searching for target genes and deciphering associated molecular pathways. However, reference gen...

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Autores principales:	Victoria S. Zucherato, Leticia B. C. Penariol, Lilian E. C. M. Silva, Cristiana C. Padovan, Omero B. Poli-Neto, Julio C. Rosa-e-Silva, Rui A. Ferriani, Juliana Meola
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7017f341bed940f695a040bdc17550dd
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spelling	oai:doaj.org-article:7017f341bed940f695a040bdc17550dd2021-12-02T15:53:00ZIdentification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis10.1038/s41598-021-84884-52045-2322https://doaj.org/article/7017f341bed940f695a040bdc17550dd2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84884-5https://doaj.org/toc/2045-2322Abstract It has been suggested that menstrual blood-derived mesenchymal stem/stromal cells (MenMSCs) are associated with the etiopathogenesis of endometriosis and considerable effort has been invested in searching for target genes and deciphering associated molecular pathways. However, reference gene stability for proper reproducible normalization in the analyses of the expression data validation is still unexplored in this experimental context. Therefore, in this exploratory study, we used stringent case and control selection criteria and collected menstrual blood from women with a laparoscopic diagnosis of advanced endometriosis and from fertile women without endometriosis. We tested for the first time the stability of 32 candidate reference genes to achieve increased accuracy and reliable results in the quantification of gene expression and direct future experiments using reverse transcription-quantitative PCR (RT-qPCR) in MenMSCs for endometriosis studies. Using the RefFinder web tool, we recommend the EIF2B1 and POP4 reference genes for the normalization of RT-qPCR data in study designs similar to ours. Furthermore, we suggest avoiding the commonly used GAPDH and ACTB reference genes as they are unstable. This high-visibility study is capable of directing different experimental designs as MenMSCs are derived from a minimally invasive tissue source with multifunctional roles in regenerative medicine.Victoria S. ZucheratoLeticia B. C. PenariolLilian E. C. M. SilvaCristiana C. PadovanOmero B. Poli-NetoJulio C. Rosa-e-SilvaRui A. FerrianiJuliana MeolaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Victoria S. Zucherato Leticia B. C. Penariol Lilian E. C. M. Silva Cristiana C. Padovan Omero B. Poli-Neto Julio C. Rosa-e-Silva Rui A. Ferriani Juliana Meola Identification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis
description	Abstract It has been suggested that menstrual blood-derived mesenchymal stem/stromal cells (MenMSCs) are associated with the etiopathogenesis of endometriosis and considerable effort has been invested in searching for target genes and deciphering associated molecular pathways. However, reference gene stability for proper reproducible normalization in the analyses of the expression data validation is still unexplored in this experimental context. Therefore, in this exploratory study, we used stringent case and control selection criteria and collected menstrual blood from women with a laparoscopic diagnosis of advanced endometriosis and from fertile women without endometriosis. We tested for the first time the stability of 32 candidate reference genes to achieve increased accuracy and reliable results in the quantification of gene expression and direct future experiments using reverse transcription-quantitative PCR (RT-qPCR) in MenMSCs for endometriosis studies. Using the RefFinder web tool, we recommend the EIF2B1 and POP4 reference genes for the normalization of RT-qPCR data in study designs similar to ours. Furthermore, we suggest avoiding the commonly used GAPDH and ACTB reference genes as they are unstable. This high-visibility study is capable of directing different experimental designs as MenMSCs are derived from a minimally invasive tissue source with multifunctional roles in regenerative medicine.
format	article
author	Victoria S. Zucherato Leticia B. C. Penariol Lilian E. C. M. Silva Cristiana C. Padovan Omero B. Poli-Neto Julio C. Rosa-e-Silva Rui A. Ferriani Juliana Meola
author_facet	Victoria S. Zucherato Leticia B. C. Penariol Lilian E. C. M. Silva Cristiana C. Padovan Omero B. Poli-Neto Julio C. Rosa-e-Silva Rui A. Ferriani Juliana Meola
author_sort	Victoria S. Zucherato
title	Identification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis
title_short	Identification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis
title_full	Identification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis
title_fullStr	Identification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis
title_full_unstemmed	Identification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis
title_sort	identification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7017f341bed940f695a040bdc17550dd
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Identification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis

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