Polymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation

Abstract Biosample encapsulation is a critical step in a wide range of biomedical and bioengineering applications. Aqueous two-phase system (ATPS) droplets have been recently introduced and showed a great promise to the biological separation and encapsulation due to their excellent biocompatibility....

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Autores principales:	Mohammad Mastiani, Negar Firoozi, Nicholas Petrozzi, Seokju Seo, Myeongsub Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7018162f0351414f937ef70da2de0403
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spelling	oai:doaj.org-article:7018162f0351414f937ef70da2de04032021-12-02T15:09:13ZPolymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation10.1038/s41598-019-51958-42045-2322https://doaj.org/article/7018162f0351414f937ef70da2de04032019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51958-4https://doaj.org/toc/2045-2322Abstract Biosample encapsulation is a critical step in a wide range of biomedical and bioengineering applications. Aqueous two-phase system (ATPS) droplets have been recently introduced and showed a great promise to the biological separation and encapsulation due to their excellent biocompatibility. This study shows for the first time the passive generation of salt-based ATPS microdroplets and their biocompatibility test. We used two ATPS including polymer/polymer (polyethylene glycol (PEG)/dextran (DEX)) and polymer/salt (PEG/Magnesium sulfate) for droplet generation in a flow-focusing geometry. Droplet morphologies and monodispersity in both systems are studied. The PEG/salt system showed an excellent capability of uniform droplet formation with a wide range of sizes (20–60 μm) which makes it a suitable candidate for encapsulation of biological samples. Therefore, we examined the potential application of the PEG/salt system for encapsulating human umbilical vein endothelial cells (HUVECs). A cell viability test was conducted on MgSO4 solutions at various concentrations and our results showed an adequate cell survival. The findings of this research suggest that the polymer/salt ATPS could be a biocompatible all-aqueous platform for cell encapsulation.Mohammad MastianiNegar FirooziNicholas PetrozziSeokju SeoMyeongsub KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammad Mastiani Negar Firoozi Nicholas Petrozzi Seokju Seo Myeongsub Kim Polymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation
description	Abstract Biosample encapsulation is a critical step in a wide range of biomedical and bioengineering applications. Aqueous two-phase system (ATPS) droplets have been recently introduced and showed a great promise to the biological separation and encapsulation due to their excellent biocompatibility. This study shows for the first time the passive generation of salt-based ATPS microdroplets and their biocompatibility test. We used two ATPS including polymer/polymer (polyethylene glycol (PEG)/dextran (DEX)) and polymer/salt (PEG/Magnesium sulfate) for droplet generation in a flow-focusing geometry. Droplet morphologies and monodispersity in both systems are studied. The PEG/salt system showed an excellent capability of uniform droplet formation with a wide range of sizes (20–60 μm) which makes it a suitable candidate for encapsulation of biological samples. Therefore, we examined the potential application of the PEG/salt system for encapsulating human umbilical vein endothelial cells (HUVECs). A cell viability test was conducted on MgSO4 solutions at various concentrations and our results showed an adequate cell survival. The findings of this research suggest that the polymer/salt ATPS could be a biocompatible all-aqueous platform for cell encapsulation.
format	article
author	Mohammad Mastiani Negar Firoozi Nicholas Petrozzi Seokju Seo Myeongsub Kim
author_facet	Mohammad Mastiani Negar Firoozi Nicholas Petrozzi Seokju Seo Myeongsub Kim
author_sort	Mohammad Mastiani
title	Polymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation
title_short	Polymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation
title_full	Polymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation
title_fullStr	Polymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation
title_full_unstemmed	Polymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation
title_sort	polymer-salt aqueous two-phase system (atps) micro-droplets for cell encapsulation
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/7018162f0351414f937ef70da2de0403
work_keys_str_mv	AT mohammadmastiani polymersaltaqueoustwophasesystematpsmicrodropletsforcellencapsulation AT negarfiroozi polymersaltaqueoustwophasesystematpsmicrodropletsforcellencapsulation AT nicholaspetrozzi polymersaltaqueoustwophasesystematpsmicrodropletsforcellencapsulation AT seokjuseo polymersaltaqueoustwophasesystematpsmicrodropletsforcellencapsulation AT myeongsubkim polymersaltaqueoustwophasesystematpsmicrodropletsforcellencapsulation
_version_	1718387880160657408

Polymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation

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