Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever

ABSTRACT Vaccine development is a top priority in malignant catarrhal fever (MCF) research. In the case of sheep-associated MCF (SA-MCF) caused by ovine herpesvirus 2 (OvHV-2), progress toward this objective has been hindered by the absence of methods to attenuate or modify the virus, since it canno...

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Autores principales:	Cristina W. Cunha, Naomi S. Taus, Benjamin G. Dewals, Alain Vanderplasschen, Donald P. Knowles, Hong Li
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Chimeric virus alcelaphine herpesvirus 1 malignant catarrhal fever ovine herpesvirus 2 vaccine Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7024625b733649da84a1e9981c34a42a
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spelling	oai:doaj.org-article:7024625b733649da84a1e9981c34a42a2021-11-15T15:21:15ZReplacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever10.1128/mSphere.00108-162379-5042https://doaj.org/article/7024625b733649da84a1e9981c34a42a2016-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00108-16https://doaj.org/toc/2379-5042ABSTRACT Vaccine development is a top priority in malignant catarrhal fever (MCF) research. In the case of sheep-associated MCF (SA-MCF) caused by ovine herpesvirus 2 (OvHV-2), progress toward this objective has been hindered by the absence of methods to attenuate or modify the virus, since it cannot be propagated in vitro. As an alternative for vaccine development, in this study, we tested the hypothesis that one of the SA-MCF vaccine candidate targets, OvHV-2 glycoprotein B (gB), could be expressed by a nonpathogenic alcelaphine herpesvirus 1 (AlHV-1) and then evaluated the potential of the AlHV-1/OvHV-2 chimera to be used as a vaccine and a diagnostic tool. The construction and characterization of an AlHV-1/OvHV-2 chimeric virus that is nonpathogenic and expresses an OvHV-2 vaccine target are significant steps toward the development of an SA-MCF vaccine and also provide a valuable means to study OvHV-2 biology.Cristina W. CunhaNaomi S. TausBenjamin G. DewalsAlain VanderplasschenDonald P. KnowlesHong LiAmerican Society for MicrobiologyarticleChimeric virusalcelaphine herpesvirus 1malignant catarrhal feverovine herpesvirus 2vaccineMicrobiologyQR1-502ENmSphere, Vol 1, Iss 4 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chimeric virus alcelaphine herpesvirus 1 malignant catarrhal fever ovine herpesvirus 2 vaccine Microbiology QR1-502
spellingShingle	Chimeric virus alcelaphine herpesvirus 1 malignant catarrhal fever ovine herpesvirus 2 vaccine Microbiology QR1-502 Cristina W. Cunha Naomi S. Taus Benjamin G. Dewals Alain Vanderplasschen Donald P. Knowles Hong Li Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever
description	ABSTRACT Vaccine development is a top priority in malignant catarrhal fever (MCF) research. In the case of sheep-associated MCF (SA-MCF) caused by ovine herpesvirus 2 (OvHV-2), progress toward this objective has been hindered by the absence of methods to attenuate or modify the virus, since it cannot be propagated in vitro. As an alternative for vaccine development, in this study, we tested the hypothesis that one of the SA-MCF vaccine candidate targets, OvHV-2 glycoprotein B (gB), could be expressed by a nonpathogenic alcelaphine herpesvirus 1 (AlHV-1) and then evaluated the potential of the AlHV-1/OvHV-2 chimera to be used as a vaccine and a diagnostic tool. The construction and characterization of an AlHV-1/OvHV-2 chimeric virus that is nonpathogenic and expresses an OvHV-2 vaccine target are significant steps toward the development of an SA-MCF vaccine and also provide a valuable means to study OvHV-2 biology.
format	article
author	Cristina W. Cunha Naomi S. Taus Benjamin G. Dewals Alain Vanderplasschen Donald P. Knowles Hong Li
author_facet	Cristina W. Cunha Naomi S. Taus Benjamin G. Dewals Alain Vanderplasschen Donald P. Knowles Hong Li
author_sort	Cristina W. Cunha
title	Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever
title_short	Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever
title_full	Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever
title_fullStr	Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever
title_full_unstemmed	Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever
title_sort	replacement of glycoprotein b in alcelaphine herpesvirus 1 by its ovine herpesvirus 2 homolog : implications in vaccine development for sheep-associated malignant catarrhal fever
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/7024625b733649da84a1e9981c34a42a
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Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever

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