Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.

<h4>Background</h4>Exosomes are small extracellular nanovesicles of endocytic origin that mediate different signals between cells, by surface interactions and by shuttling functional RNA from one cell to another. Exosomes are released by many cells including mast cells, dendritic cells,...

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Autores principales:	Maria Eldh, Karin Ekström, Hadi Valadi, Margareta Sjöstrand, Bob Olsson, Margareta Jernås, Jan Lötvall
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7026957562f54fe8a09d3a8778d8a62c
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spelling	oai:doaj.org-article:7026957562f54fe8a09d3a8778d8a62c2021-11-18T07:01:34ZExosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.1932-620310.1371/journal.pone.0015353https://doaj.org/article/7026957562f54fe8a09d3a8778d8a62c2010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21179422/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Exosomes are small extracellular nanovesicles of endocytic origin that mediate different signals between cells, by surface interactions and by shuttling functional RNA from one cell to another. Exosomes are released by many cells including mast cells, dendritic cells, macrophages, epithelial cells and tumour cells. Exosomes differ compared to their donor cells, not only in size, but also in their RNA, protein and lipid composition.<h4>Methodology/principal findings</h4>In this study, we show that exosomes, released by mouse mast cells exposed to oxidative stress, differ in their mRNA content. Also, we show that these exosomes can influence the response of other cells to oxidative stress by providing recipient cells with a resistance against oxidative stress, observed as an attenuated loss of cell viability. Furthermore, Affymetrix microarray analysis revealed that the exosomal mRNA content not only differs between exosomes and donor cells, but also between exosomes derived from cells grown under different conditions; oxidative stress and normal conditions. Finally, we also show that exposure to UV-light affects the biological functions associated with exosomes released under oxidative stress.<h4>Conclusions/significance</h4>These results argue that the exosomal shuttle of RNA is involved in cell-to-cell communication, by influencing the response of recipient cells to an external stress stimulus.Maria EldhKarin EkströmHadi ValadiMargareta SjöstrandBob OlssonMargareta JernåsJan LötvallPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e15353 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Eldh Karin Ekström Hadi Valadi Margareta Sjöstrand Bob Olsson Margareta Jernås Jan Lötvall Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.
description	<h4>Background</h4>Exosomes are small extracellular nanovesicles of endocytic origin that mediate different signals between cells, by surface interactions and by shuttling functional RNA from one cell to another. Exosomes are released by many cells including mast cells, dendritic cells, macrophages, epithelial cells and tumour cells. Exosomes differ compared to their donor cells, not only in size, but also in their RNA, protein and lipid composition.<h4>Methodology/principal findings</h4>In this study, we show that exosomes, released by mouse mast cells exposed to oxidative stress, differ in their mRNA content. Also, we show that these exosomes can influence the response of other cells to oxidative stress by providing recipient cells with a resistance against oxidative stress, observed as an attenuated loss of cell viability. Furthermore, Affymetrix microarray analysis revealed that the exosomal mRNA content not only differs between exosomes and donor cells, but also between exosomes derived from cells grown under different conditions; oxidative stress and normal conditions. Finally, we also show that exposure to UV-light affects the biological functions associated with exosomes released under oxidative stress.<h4>Conclusions/significance</h4>These results argue that the exosomal shuttle of RNA is involved in cell-to-cell communication, by influencing the response of recipient cells to an external stress stimulus.
format	article
author	Maria Eldh Karin Ekström Hadi Valadi Margareta Sjöstrand Bob Olsson Margareta Jernås Jan Lötvall
author_facet	Maria Eldh Karin Ekström Hadi Valadi Margareta Sjöstrand Bob Olsson Margareta Jernås Jan Lötvall
author_sort	Maria Eldh
title	Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.
title_short	Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.
title_full	Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.
title_fullStr	Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.
title_full_unstemmed	Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.
title_sort	exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle rna.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/7026957562f54fe8a09d3a8778d8a62c
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Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.

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