Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity

Infectious bovine rhinotracheitis (IBR), caused by bovine alphaherpesvirus 1 (BoHV-1), is an important disease affecting cattle worldwide resulting in great economic losses. Marker vaccines are effective in controlling infectious diseases including IBR, because they allow the discrimination between...

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Autores principales:	Marawan A. Marawan, Mingliang Deng, Chen Wang, Yingyu Chen, Changmin Hu, Jianguo Chen, Xi Chen, Huanchun Chen, Aizhen Guo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bovine herpesvirus 1,5 proteomics glycoprotein marker vaccines virulence immunity Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/702d3ae7020849d78c92a325c4fe0e4a
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spelling	oai:doaj.org-article:702d3ae7020849d78c92a325c4fe0e4a2021-11-25T19:11:54ZCharacterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity10.3390/vetsci81102532306-7381https://doaj.org/article/702d3ae7020849d78c92a325c4fe0e4a2021-10-01T00:00:00Zhttps://www.mdpi.com/2306-7381/8/11/253https://doaj.org/toc/2306-7381Infectious bovine rhinotracheitis (IBR), caused by bovine alphaherpesvirus 1 (BoHV-1), is an important disease affecting cattle worldwide resulting in great economic losses. Marker vaccines are effective in controlling infectious diseases including IBR, because they allow the discrimination between the natural infection and the vaccination. Therefore, a triple gene deleted strain BoHV-1 gG-/tk-/gE- was developed and evaluated in vivo and in vitro as a marker vaccine. In cell culture, this triple mutant virus showed significantly slower growth kinetics and smaller plaques when compared to wild-type (wt) BoHV-1 and double mutant BoHV-1 gG-/tk- (<i>p</i> < 0.01). On proteomic level, it revealed downregulation of some virulence related proteins including thymidine kinase, glycoproteins G, E, I, and K when compared to the wt. In vitro, the triple mutant virus showed a significantly lower and shorter viral shedding period (<i>p</i> < 0.001) in calves compared to double mutant. Moreover, the immunized calves with triple mutant virus showed protection rates of 64.2% and 68.6% against wt BoHV-1 and wt BoHV-5 challenge, respectively, without reactivation of latency after dexamethasone injection. In conclusion, BoHV-1 gG-/tk-/gE- is a safer marker vaccine against IBR although its immunogenicity in calves was decreased when compared to double mutant virus.Marawan A. MarawanMingliang DengChen WangYingyu ChenChangmin HuJianguo ChenXi ChenHuanchun ChenAizhen GuoMDPI AGarticlebovine herpesvirus 1,5proteomicsglycoproteinmarker vaccinesvirulenceimmunityVeterinary medicineSF600-1100ENVeterinary Sciences, Vol 8, Iss 253, p 253 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine herpesvirus 1,5 proteomics glycoprotein marker vaccines virulence immunity Veterinary medicine SF600-1100
spellingShingle	bovine herpesvirus 1,5 proteomics glycoprotein marker vaccines virulence immunity Veterinary medicine SF600-1100 Marawan A. Marawan Mingliang Deng Chen Wang Yingyu Chen Changmin Hu Jianguo Chen Xi Chen Huanchun Chen Aizhen Guo Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity
description	Infectious bovine rhinotracheitis (IBR), caused by bovine alphaherpesvirus 1 (BoHV-1), is an important disease affecting cattle worldwide resulting in great economic losses. Marker vaccines are effective in controlling infectious diseases including IBR, because they allow the discrimination between the natural infection and the vaccination. Therefore, a triple gene deleted strain BoHV-1 gG-/tk-/gE- was developed and evaluated in vivo and in vitro as a marker vaccine. In cell culture, this triple mutant virus showed significantly slower growth kinetics and smaller plaques when compared to wild-type (wt) BoHV-1 and double mutant BoHV-1 gG-/tk- (<i>p</i> < 0.01). On proteomic level, it revealed downregulation of some virulence related proteins including thymidine kinase, glycoproteins G, E, I, and K when compared to the wt. In vitro, the triple mutant virus showed a significantly lower and shorter viral shedding period (<i>p</i> < 0.001) in calves compared to double mutant. Moreover, the immunized calves with triple mutant virus showed protection rates of 64.2% and 68.6% against wt BoHV-1 and wt BoHV-5 challenge, respectively, without reactivation of latency after dexamethasone injection. In conclusion, BoHV-1 gG-/tk-/gE- is a safer marker vaccine against IBR although its immunogenicity in calves was decreased when compared to double mutant virus.
format	article
author	Marawan A. Marawan Mingliang Deng Chen Wang Yingyu Chen Changmin Hu Jianguo Chen Xi Chen Huanchun Chen Aizhen Guo
author_facet	Marawan A. Marawan Mingliang Deng Chen Wang Yingyu Chen Changmin Hu Jianguo Chen Xi Chen Huanchun Chen Aizhen Guo
author_sort	Marawan A. Marawan
title	Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity
title_short	Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity
title_full	Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity
title_fullStr	Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity
title_full_unstemmed	Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity
title_sort	characterization of bohv-1 gg-/tk-/ge- mutant in differential protein expression, virulence, and immunity
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/702d3ae7020849d78c92a325c4fe0e4a
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Characterization of BoHV-1 gG-/tk-/gE- Mutant in Differential Protein Expression, Virulence, and Immunity

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