Identification of rabbit annulus fibrosus-derived stem cells.

Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biome...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen Liu, Qianping Guo, Jun Li, Shenghao Wang, Yibin Wang, Bin Li, Huilin Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/704929f66fea433db292f980b6464e77
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:704929f66fea433db292f980b6464e77
record_format	dspace
spelling	oai:doaj.org-article:704929f66fea433db292f980b6464e772021-11-25T05:59:06ZIdentification of rabbit annulus fibrosus-derived stem cells.1932-620310.1371/journal.pone.0108239https://doaj.org/article/704929f66fea433db292f980b6464e772014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108239https://doaj.org/toc/1932-6203Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biomechanical levels. In this study, we isolated and characterized a sub-population of cells from rabbit AF tissue which formed colonies in vitro and could self-renew. These cells showed gene expression of typical surface antigen molecules characterizing mesenchymal stem cells (MSCs), including CD29, CD44, and CD166. Meanwhile, they did not express negative markers of MSCs such as CD4, CD8, and CD14. They also expressed Oct-4, nucleostemin, and SSEA-4 proteins. Upon induced differentiation they showed typical osteogenesis, chondrogenesis, and adipogenesis potential. Together, these AF-derived colony-forming cells possessed clonogenicity, self-renewal, and multi-potential differentiation capability, the three criteria characterizing MSCs. Such AF-derived stem cells may potentially be an ideal candidate for DDD treatments using cell therapies or tissue engineering approaches.Chen LiuQianping GuoJun LiShenghao WangYibin WangBin LiHuilin YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108239 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chen Liu Qianping Guo Jun Li Shenghao Wang Yibin Wang Bin Li Huilin Yang Identification of rabbit annulus fibrosus-derived stem cells.
description	Annulus fibrosus (AF) injuries can lead to substantial deterioration of intervertebral disc (IVD) which characterizes degenerative disc disease (DDD). However, treatments for AF repair/regeneration remain challenging due to the intrinsic heterogeneity of AF tissue at cellular, biochemical, and biomechanical levels. In this study, we isolated and characterized a sub-population of cells from rabbit AF tissue which formed colonies in vitro and could self-renew. These cells showed gene expression of typical surface antigen molecules characterizing mesenchymal stem cells (MSCs), including CD29, CD44, and CD166. Meanwhile, they did not express negative markers of MSCs such as CD4, CD8, and CD14. They also expressed Oct-4, nucleostemin, and SSEA-4 proteins. Upon induced differentiation they showed typical osteogenesis, chondrogenesis, and adipogenesis potential. Together, these AF-derived colony-forming cells possessed clonogenicity, self-renewal, and multi-potential differentiation capability, the three criteria characterizing MSCs. Such AF-derived stem cells may potentially be an ideal candidate for DDD treatments using cell therapies or tissue engineering approaches.
format	article
author	Chen Liu Qianping Guo Jun Li Shenghao Wang Yibin Wang Bin Li Huilin Yang
author_facet	Chen Liu Qianping Guo Jun Li Shenghao Wang Yibin Wang Bin Li Huilin Yang
author_sort	Chen Liu
title	Identification of rabbit annulus fibrosus-derived stem cells.
title_short	Identification of rabbit annulus fibrosus-derived stem cells.
title_full	Identification of rabbit annulus fibrosus-derived stem cells.
title_fullStr	Identification of rabbit annulus fibrosus-derived stem cells.
title_full_unstemmed	Identification of rabbit annulus fibrosus-derived stem cells.
title_sort	identification of rabbit annulus fibrosus-derived stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/704929f66fea433db292f980b6464e77
work_keys_str_mv	AT chenliu identificationofrabbitannulusfibrosusderivedstemcells AT qianpingguo identificationofrabbitannulusfibrosusderivedstemcells AT junli identificationofrabbitannulusfibrosusderivedstemcells AT shenghaowang identificationofrabbitannulusfibrosusderivedstemcells AT yibinwang identificationofrabbitannulusfibrosusderivedstemcells AT binli identificationofrabbitannulusfibrosusderivedstemcells AT huilinyang identificationofrabbitannulusfibrosusderivedstemcells
_version_	1718414346783031296

Identification of rabbit annulus fibrosus-derived stem cells.

Ejemplares similares